Capability of Fluorescent Capillary Electrophoresis To Distinguish Species of the Candida parapsilosis Complex

KOTÁSKOVÁ, Iva, Hana OBRUČOVÁ, Veronika LÝČKOVÁ, Filip RŮŽIČKA and Tomáš FREIBERGER. Capability of Fluorescent Capillary Electrophoresis To Distinguish Species of the Candida parapsilosis Complex. Online. Journal of Clinical Microbiology. Washington: American Society for Microbiology, 2019, vol. 57, No 5, p. 1-3. ISSN 0095-1137. Available from: https://dx.doi.org/10.1128/JCM.00135-19. [citováno 2024-04-24]

Basic information

• Original name: Capability of Fluorescent Capillary Electrophoresis To Distinguish Species of the Candida parapsilosis Complex
• Authors: KOTÁSKOVÁ, Iva (203 Czech Republic, belonging to the institution), Hana OBRUČOVÁ (203 Czech Republic), Veronika LÝČKOVÁ (203 Czech Republic, belonging to the institution), Filip RŮŽIČKA (203 Czech Republic, belonging to the institution) and Tomáš FREIBERGER (203 Czech Republic, guarantor, belonging to the institution)
• Edition: Journal of Clinical Microbiology, Washington, American Society for Microbiology, 2019, 0095-1137.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10606 Microbiology
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 5.897
• RIV identification code: RIV/00216224:14110/19:00108483
• Organization unit: Faculty of Medicine
• Doi: http://dx.doi.org/10.1128/JCM.00135-19
• UT WoS: 000466042600008
• Keywords in English: Candida; Candida parapsilosis complex; MALDI-TOF; fluorescent capillary electrophoresis; fungi
• Tags: 14110113, 14110114, rivok
• Tags: International impact, Reviewed

Abstract

Candida parapsilosis complex (psilosis complex) has three genetically distinct but not easily phenotypically distinguishable cryptic species, C. parapsilosis sensu stricto, Candida metapsilosis, and C. orthopsilosis. It has been reported as one of the most important non-albicans agents commonly detected in invasive candidiasis (1–3). Each psilosis complex species manifests a unique epidemiology, virulence, and antifungal susceptibility (4); thus, precise identification can be of clinical interest, and various molecular methods are being developed (5–7). Our previous studies (5, 8) reported the capability of ITS2 ribosomal DNA (rDNA) amplification followed by fluorescent capillary electrophoresis (f-ITS2-PCR-CE) to distinguish between various Candida species, including psilosis complex, by a small but constant 0.4-bp difference between C. parapsilosis and Candida orthopsilosis. The actual positions of the size standard fragments are plotted against the expected size of each size standard fragment, which defines the function of these variables. The size (bp) of an analyzed fragment is then calculated according to this function; therefore, decimal values are being reported. The 0.4-bp size difference is presumably caused by the different mobilities of singlestranded DNA (ssDNA) fragments, resulting from their different primary structures.

Links

• MUNI/A/0925/2017, interní kód MU: Name: Poruchy tvorby protilátek a komplementového systému

Investor: Masaryk University, Category A

• MUNI/A/1189/2018, interní kód MU: Name: Původci nozokomiálních nákaz a možnosti jejich diagnostiky a terapie (Acronym: PNNMDT)

Investor: Masaryk University, Category A

• MUNI/A/1298/2018, interní kód MU: Name: Vrozené a získané deficience imunitního systému (Acronym: Poruchy imunity)

Investor: Masaryk University, Category A

• NV16-31593A, research and development project: Name: Polymikrobiální biofilm jako komplexní mikrobiom v rámci léčby nozokomiálních infekcí