J 2019

Capability of Fluorescent Capillary Electrophoresis To Distinguish Species of the Candida parapsilosis Complex

KOTÁSKOVÁ, Iva, Hana OBRUČOVÁ, Veronika LÝČKOVÁ, Filip RŮŽIČKA, Tomáš FREIBERGER et. al.

Basic information

Original name

Capability of Fluorescent Capillary Electrophoresis To Distinguish Species of the Candida parapsilosis Complex

Authors

KOTÁSKOVÁ, Iva (203 Czech Republic, belonging to the institution), Hana OBRUČOVÁ (203 Czech Republic), Veronika LÝČKOVÁ (203 Czech Republic, belonging to the institution), Filip RŮŽIČKA (203 Czech Republic, belonging to the institution) and Tomáš FREIBERGER (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Clinical Microbiology, Washington, American Society for Microbiology, 2019, 0095-1137

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 5.897

RIV identification code

RIV/00216224:14110/19:00108483

Organization unit

Faculty of Medicine

DOI

UT WoS

000466042600008

Keywords in English

Candida; Candida parapsilosis complex; MALDI-TOF; fluorescent capillary electrophoresis; fungi

Tags

Tags

International impact, Reviewed
Změněno: 4/6/2020 08:39, Mgr. Tereza Miškechová

Abstract

V originále

Candida parapsilosis complex (psilosis complex) has three genetically distinct but not easily phenotypically distinguishable cryptic species, C. parapsilosis sensu stricto, Candida metapsilosis, and C. orthopsilosis. It has been reported as one of the most important non-albicans agents commonly detected in invasive candidiasis (1–3). Each psilosis complex species manifests a unique epidemiology, virulence, and antifungal susceptibility (4); thus, precise identification can be of clinical interest, and various molecular methods are being developed (5–7). Our previous studies (5, 8) reported the capability of ITS2 ribosomal DNA (rDNA) amplification followed by fluorescent capillary electrophoresis (f-ITS2-PCR-CE) to distinguish between various Candida species, including psilosis complex, by a small but constant 0.4-bp difference between C. parapsilosis and Candida orthopsilosis. The actual positions of the size standard fragments are plotted against the expected size of each size standard fragment, which defines the function of these variables. The size (bp) of an analyzed fragment is then calculated according to this function; therefore, decimal values are being reported. The 0.4-bp size difference is presumably caused by the different mobilities of singlestranded DNA (ssDNA) fragments, resulting from their different primary structures.

Links

MUNI/A/0925/2017, interní kód MU
Name: Poruchy tvorby protilátek a komplementového systému
Investor: Masaryk University, Category A
MUNI/A/1189/2018, interní kód MU
Name: Původci nozokomiálních nákaz a možnosti jejich diagnostiky a terapie (Acronym: PNNMDT)
Investor: Masaryk University, Category A
MUNI/A/1298/2018, interní kód MU
Name: Vrozené a získané deficience imunitního systému (Acronym: Poruchy imunity)
Investor: Masaryk University, Category A
NV16-31593A, research and development project
Name: Polymikrobiální biofilm jako komplexní mikrobiom v rámci léčby nozokomiálních infekcí