Nematodes as a tool to study insect immunity

HYRŠL, Pavel, Pavel DOBEŠ, Martin KUNC and Jana HURYCHOVÁ. Nematodes as a tool to study insect immunity. In Frontiers in insect physiology. 2019.

Basic information

• Original name: Nematodes as a tool to study insect immunity
• Name in Czech: Hlístice jako nástroj pro studium imunity hmyzu
• Name (in English): Nematodes as a tool to study insect immunity
• Authors: HYRŠL, Pavel (203 Czech Republic, guarantor, belonging to the institution), Pavel DOBEŠ (203 Czech Republic, belonging to the institution), Martin KUNC (203 Czech Republic, belonging to the institution) and Jana HURYCHOVÁ (203 Czech Republic, belonging to the institution).
• Edition: Frontiers in insect physiology, 2019.

Other information

• Original language: Czech
• Type of outcome: Conference abstract
• Field of Study: 40301 Veterinary science
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/19:00107461
• Organization unit: Faculty of Science
• Keywords (in Czech): entomoaptogenní hlístice; imunita hmyzu; Heterorhabditis; Steinernema; Galleria; Drosophila
• Keywords in English: entomopathogenic nematodes; insect immunity; Heterorhabditis; Steinernema; Galleria; Drosophila

Abstract

Invertebrates and especially insects belong to the ecologically most successful organisms living on Earth. An adaptation to the antigen pressure of the environment (mainly to micro-organisms) depends on insect innate immunity. Invertebrates compensated the absence of complicated immune reactions by specific adaptations and functions of cellular and humoral parts of their immune system. Although an adaptive immunity in the form we know in vertebrates does not exist in invertebrates, there are advanced mechanisms modulating their immune response. Presented studies on fruit fly Drosophila melanogaster, wax moth Galleria mellonella and honey bee Apis mellifera describe cellular and humoral components of their immune system and methods for their measurement. In many experiments we used natural infection model combining two pathogens – bacteria Photorhabdus luminescens and nematode Heterorhabditis bacteriophora with their insect host. New mechanisms of insect immune response to nematobacterial pathogens were identified. Not surprisingly, among the genes significantly affected by the nematobacterial infection, mostly those related to immunity, cellular and developmental processes were found to be crucial, e.g. genes coding for members of coagulation cascade and recognition molecules. This study was supported by grant GAČR 17 - 03253S.

Abstract (in English)

Invertebrates and especially insects belong to the ecologically most successful organisms living on Earth. An adaptation to the antigen pressure of the environment (mainly to micro-organisms) depends on insect innate immunity. Invertebrates compensated the absence of complicated immune reactions by specific adaptations and functions of cellular and humoral parts of their immune system. Although an adaptive immunity in the form we know in vertebrates does not exist in invertebrates, there are advanced mechanisms modulating their immune response. Presented studies on fruit fly Drosophila melanogaster, wax moth Galleria mellonella and honey bee Apis mellifera describe cellular and humoral components of their immune system and methods for their measurement. In many experiments we used natural infection model combining two pathogens – bacteria Photorhabdus luminescens and nematode Heterorhabditis bacteriophora with their insect host. New mechanisms of insect immune response to nematobacterial pathogens were identified. Not surprisingly, among the genes significantly affected by the nematobacterial infection, mostly those related to immunity, cellular and developmental processes were found to be crucial, e.g. genes coding for members of coagulation cascade and recognition molecules. This study was supported by grant GAČR 17 - 03253S.

Links

• GA17-03253S, research and development project: Name: Hormonální kontrola hmyzího obranného systému

Investor: Czech Science Foundation