Ampérometrický biosenzor pre rýchle stanovenie albumínu v moči
| ŠIŠKOVÁ, Martina, Matěj PASTUCHA, Marta FARKOVÁ, Přemysl LUBAL and Zdeněk FARKA. Ampérometrický biosenzor pre rýchle stanovenie albumínu v moči (Amperometric Biosensor for Rapid Determination of Albumin in the Urine). In 22. ročník celostátní soutěže o nejlepší studentskou vědeckou práci v oboru analytická chemie Cena Karla Štulíka 2019. 2019. ISSN 2336-7202. |
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| Basic information | |
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| Original name | Ampérometrický biosenzor pre rýchle stanovenie albumínu v moči |
| Name (in English) | Amperometric Biosensor for Rapid Determination of Albumin in the Urine |
| Authors | ŠIŠKOVÁ, Martina, Matěj PASTUCHA, Marta FARKOVÁ, Přemysl LUBAL and Zdeněk FARKA. |
| Edition | 22. ročník celostátní soutěže o nejlepší studentskou vědeckou práci v oboru analytická chemie Cena Karla Štulíka 2019, 2019. |
| Other information | |
|---|---|
| Original language | Slovak |
| Type of outcome | Conference abstract |
| Field of Study | 10406 Analytical chemistry |
| Country of publisher | Czech Republic |
| Confidentiality degree | is not subject to a state or trade secret |
| WWW | URL |
| Organization unit | Central European Institute of Technology |
| ISSN | 2336-7202 |
| Keywords in English | amperometry; human serum albumin; biosensor |
| Changed by | Changed by: doc. Mgr. Zdeněk Farka, Ph.D., učo 357740. Changed: 27/7/2019 16:11. |
| Abstract (in English) |
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| This work presents the development of an amperometric biosensor for the rapid determination of clinically relevant concentrations of human serum albumin in the urine, whose increased concentration indicates nephropathy. This simple method is characterized by a short analysis time, low cost of determination, possibility of miniaturization, automatization and point of care analysis. The assay was performed by using various methods of immobilization the antibody to screen-printed electrodes by means of mixed and flow-through configuration. By immobilizing the antibody via cysteamine and glutaraldehyde in a mixed analysis, a detection limit of 10 ng ml–1 was reached, allowing the determination of human serum albumin in the urine. |
| Links | |
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| LQ1601, research and development project | Name: CEITEC 2020 (Acronym: CEITEC2020) |
| Investor: Ministry of Education, Youth and Sports of the CR | |
| MUNI/A/1237/2016, interní kód MU | Name: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2017 |
| Investor: Masaryk University, Category A | |
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