Immunohistochemical analysis of retraction pocket pars tensa of
tympanic membrane in children

J 2019

Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children

URÍK, Milan; Pavel HURNIK; Dušan ŽIAK; Josef MACHAČ; Ivo ŠLAPÁK et. al.

Basic information

Original name

Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children

Authors

URÍK, Milan (703 Slovakia, guarantor, belonging to the institution); Pavel HURNIK (203 Czech Republic); Dušan ŽIAK (203 Czech Republic); Josef MACHAČ (203 Czech Republic, belonging to the institution); Ivo ŠLAPÁK (203 Czech Republic, belonging to the institution); Oldřich MOTYKA (203 Czech Republic) and Petr JABANDŽIEV (203 Czech Republic, belonging to the institution)

Edition

International journal of pediatric otorhinolaryngology, Amsterdam, Elsevier, 2019, 0165-5876

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

30206 Otorhinolaryngology

Country of publisher

Ireland

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 1.241

RIV identification code

RIV/00216224:14110/19:00110123

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.ijporl.2019.04.008

UT WoS

000471738900019

EID Scopus

2-s2.0-85064229124

Keywords in English

Retraction pocket; Cholesteatoma; Immunohistochemistry; Chidren; Pars tensa

Abstract

V originále

Aims: Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children. Identification of signs typical for cholesteatoma and support of retraction theory of cholesteatoma. Study design: a prospective study analysing 31 surgically removed retraction pockets. Department: University Hospital, Children's Medical Centre Methods: Retraction pockets processed by a standard process for immunohistochemical analysis. The observed findings were specified using antibodies CD45 LCA (leukocyte common antigen), CD31 (platelet endothelial cell adhesion molecule), D2-40 (marker of lymphatic endothelium), MMP9 (marker of degradation of connective tissue extracellular matrix) and Ki67 (cellular marker of proliferation). Results: All observed parameters except for MMP9 had a significantly higher incidence in retraction pocket stage III compared to stage II according to Charachon. Conclusion: We described immunohistochemical signs of retraction pocket pars tensa of tympanic membrane in children resulting in cholesteatoma. All the observed signs occur in the structure of matrix and perimatrix of cholesteatoma. A significantly higher incidence of all observed parameters except from MMP9 was proved in retraction pocket stage III, unlike in stage II. This observation proves the fact that retraction pocket is a progressive disease and is a procholesteatoma stage.

Links

MUNI/A/1002/2018, interní kód MU

Name: Ztráta sluchu v dětském věku - příčiny, diagnostika, možnosti rehabilitace III

Investor: Masaryk University, Category A

Cite

URÍK, Milan; Pavel HURNIK; Dušan ŽIAK; Josef MACHAČ; Ivo ŠLAPÁK; Oldřich MOTYKA and Petr JABANDŽIEV. Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children. International journal of pediatric otorhinolaryngology. Amsterdam: Elsevier, 2019, vol. 122, JUL 2019, p. 111-116. ISSN 0165-5876. Available from: https://dx.doi.org/10.1016/j.ijporl.2019.04.008.

@article{1545096,
   author = {Urík, Milan and Hurnik, Pavel and Žiak, Dušan and Machač, Josef and Šlapák, Ivo and Motyka, Oldřich and Jabandžiev, Petr},
   article_location = {Amsterdam},
   article_number = {JUL 2019},
   doi = {http://dx.doi.org/10.1016/j.ijporl.2019.04.008},
   keywords = {Retraction pocket; Cholesteatoma; Immunohistochemistry; Chidren; Pars tensa},
   language = {eng},
   issn = {0165-5876},
   journal = {International journal of pediatric otorhinolaryngology},
   title = {Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children},
   url = {http://dx.doi.org/10.1016/j.ijporl.2019.04.008},
   volume = {122},
   year = {2019}
}

TY  - JOUR
ID  - 1545096
AU  - Urík, Milan - Hurnik, Pavel - Žiak, Dušan - Machač, Josef - Šlapák, Ivo - Motyka, Oldřich - Jabandžiev, Petr
PY  - 2019
TI  - Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children
JF  - International journal of pediatric otorhinolaryngology
VL  - 122
IS  - JUL 2019
SP  - 111-116
EP  - 111-116
PB  - Elsevier
SN  - 01655876
KW  - Retraction pocket
KW  - Cholesteatoma
KW  - Immunohistochemistry
KW  - Chidren
KW  - Pars tensa
UR  - http://dx.doi.org/10.1016/j.ijporl.2019.04.008
L2  - http://dx.doi.org/10.1016/j.ijporl.2019.04.008
N2  - Aims: Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children. Identification of signs typical for cholesteatoma and support of retraction theory of cholesteatoma. Study design: a prospective study analysing 31 surgically removed retraction pockets. Department: University Hospital, Children's Medical Centre Methods: Retraction pockets processed by a standard process for immunohistochemical analysis. The observed findings were specified using antibodies CD45 LCA (leukocyte common antigen), CD31 (platelet endothelial cell adhesion molecule), D2-40 (marker of lymphatic endothelium), MMP9 (marker of degradation of connective tissue extracellular matrix) and Ki67 (cellular marker of proliferation). Results: All observed parameters except for MMP9 had a significantly higher incidence in retraction pocket stage III compared to stage II according to Charachon. Conclusion: We described immunohistochemical signs of retraction pocket pars tensa of tympanic membrane in children resulting in cholesteatoma. All the observed signs occur in the structure of matrix and perimatrix of cholesteatoma. A significantly higher incidence of all observed parameters except from MMP9 was proved in retraction pocket stage III, unlike in stage II. This observation proves the fact that retraction pocket is a progressive disease and is a procholesteatoma stage.
ER  -

URÍK, Milan; Pavel HURNIK; Dušan ŽIAK; Josef MACHAČ; Ivo ŠLAPÁK; Oldřich MOTYKA and Petr JABANDŽIEV. Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children. \textit{International journal of pediatric otorhinolaryngology}. Amsterdam: Elsevier, 2019, vol.~122, JUL 2019, p.~111-116. ISSN~0165-5876. Available from: https://dx.doi.org/10.1016/j.ijporl.2019.04.008.

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