URÍK, Milan, Pavel HURNIK, Dušan ŽIAK, Josef MACHAČ, Ivo ŠLAPÁK, Oldřich MOTYKA and Petr JABANDŽIEV. Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children. International journal of pediatric otorhinolaryngology. Amsterdam: Elsevier, 2019, vol. 122, JUL 2019, p. 111-116. ISSN 0165-5876. Available from: https://dx.doi.org/10.1016/j.ijporl.2019.04.008. |
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@article{1545096, author = {Urík, Milan and Hurnik, Pavel and Žiak, Dušan and Machač, Josef and Šlapák, Ivo and Motyka, Oldřich and Jabandžiev, Petr}, article_location = {Amsterdam}, article_number = {JUL 2019}, doi = {http://dx.doi.org/10.1016/j.ijporl.2019.04.008}, keywords = {Retraction pocket; Cholesteatoma; Immunohistochemistry; Chidren; Pars tensa}, language = {eng}, issn = {0165-5876}, journal = {International journal of pediatric otorhinolaryngology}, title = {Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children}, url = {http://dx.doi.org/10.1016/j.ijporl.2019.04.008}, volume = {122}, year = {2019} }
TY - JOUR ID - 1545096 AU - Urík, Milan - Hurnik, Pavel - Žiak, Dušan - Machač, Josef - Šlapák, Ivo - Motyka, Oldřich - Jabandžiev, Petr PY - 2019 TI - Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children JF - International journal of pediatric otorhinolaryngology VL - 122 IS - JUL 2019 SP - 111-116 EP - 111-116 PB - Elsevier SN - 01655876 KW - Retraction pocket KW - Cholesteatoma KW - Immunohistochemistry KW - Chidren KW - Pars tensa UR - http://dx.doi.org/10.1016/j.ijporl.2019.04.008 L2 - http://dx.doi.org/10.1016/j.ijporl.2019.04.008 N2 - Aims: Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children. Identification of signs typical for cholesteatoma and support of retraction theory of cholesteatoma. Study design: a prospective study analysing 31 surgically removed retraction pockets. Department: University Hospital, Children's Medical Centre Methods: Retraction pockets processed by a standard process for immunohistochemical analysis. The observed findings were specified using antibodies CD45 LCA (leukocyte common antigen), CD31 (platelet endothelial cell adhesion molecule), D2-40 (marker of lymphatic endothelium), MMP9 (marker of degradation of connective tissue extracellular matrix) and Ki67 (cellular marker of proliferation). Results: All observed parameters except for MMP9 had a significantly higher incidence in retraction pocket stage III compared to stage II according to Charachon. Conclusion: We described immunohistochemical signs of retraction pocket pars tensa of tympanic membrane in children resulting in cholesteatoma. All the observed signs occur in the structure of matrix and perimatrix of cholesteatoma. A significantly higher incidence of all observed parameters except from MMP9 was proved in retraction pocket stage III, unlike in stage II. This observation proves the fact that retraction pocket is a progressive disease and is a procholesteatoma stage. ER -
URÍK, Milan, Pavel HURNIK, Dušan ŽIAK, Josef MACHAČ, Ivo ŠLAPÁK, Oldřich MOTYKA and Petr JABANDŽIEV. Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children. \textit{International journal of pediatric otorhinolaryngology}. Amsterdam: Elsevier, 2019, vol.~122, JUL 2019, p.~111-116. ISSN~0165-5876. Available from: https://dx.doi.org/10.1016/j.ijporl.2019.04.008.
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