Detailed Information on Publication Record
2019
Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children
URÍK, Milan, Pavel HURNIK, Dušan ŽIAK, Josef MACHAČ, Ivo ŠLAPÁK et. al.Basic information
Original name
Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children
Authors
URÍK, Milan (703 Slovakia, guarantor, belonging to the institution), Pavel HURNIK (203 Czech Republic), Dušan ŽIAK (203 Czech Republic), Josef MACHAČ (203 Czech Republic, belonging to the institution), Ivo ŠLAPÁK (203 Czech Republic, belonging to the institution), Oldřich MOTYKA (203 Czech Republic) and Petr JABANDŽIEV (203 Czech Republic, belonging to the institution)
Edition
International journal of pediatric otorhinolaryngology, Amsterdam, Elsevier, 2019, 0165-5876
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30206 Otorhinolaryngology
Country of publisher
Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 1.241
RIV identification code
RIV/00216224:14110/19:00110123
Organization unit
Faculty of Medicine
UT WoS
000471738900019
Keywords in English
Retraction pocket; Cholesteatoma; Immunohistochemistry; Chidren; Pars tensa
Změněno: 10/7/2019 11:25, Soňa Böhmová
Abstract
V originále
Aims: Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children. Identification of signs typical for cholesteatoma and support of retraction theory of cholesteatoma. Study design: a prospective study analysing 31 surgically removed retraction pockets. Department: University Hospital, Children's Medical Centre Methods: Retraction pockets processed by a standard process for immunohistochemical analysis. The observed findings were specified using antibodies CD45 LCA (leukocyte common antigen), CD31 (platelet endothelial cell adhesion molecule), D2-40 (marker of lymphatic endothelium), MMP9 (marker of degradation of connective tissue extracellular matrix) and Ki67 (cellular marker of proliferation). Results: All observed parameters except for MMP9 had a significantly higher incidence in retraction pocket stage III compared to stage II according to Charachon. Conclusion: We described immunohistochemical signs of retraction pocket pars tensa of tympanic membrane in children resulting in cholesteatoma. All the observed signs occur in the structure of matrix and perimatrix of cholesteatoma. A significantly higher incidence of all observed parameters except from MMP9 was proved in retraction pocket stage III, unlike in stage II. This observation proves the fact that retraction pocket is a progressive disease and is a procholesteatoma stage.
Links
| MUNI/A/1002/2018, interní kód MU |
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