| JANOVIČ, Tomáš, Martin STOJASPAL, Pavel VEVERKA, Denisa HORÁKOVÁ and Ctirad HOFR. Human Telomere Repeat Binding Factor TRF1 Replaces TRF2 Bound to Shelterin Core Hub TIN2 when TPP1 Is Absent. Journal of Molecular Biology. London: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2019, vol. 431, No 17, p. 3289-3301. ISSN 0022-2836. Available from: https://dx.doi.org/10.1016/j.jmb.2019.05.038. |
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@article{1545278,
author = {Janovič, Tomáš and Stojaspal, Martin and Veverka, Pavel and Horáková, Denisa and Hofr, Ctirad},
article_location = {London},
article_number = {17},
doi = {http://dx.doi.org/10.1016/j.jmb.2019.05.038},
keywords = {TIN2; telomere; shelterin; assembly; single-molecule},
language = {eng},
issn = {0022-2836},
journal = {Journal of Molecular Biology},
title = {Human Telomere Repeat Binding Factor TRF1 Replaces TRF2 Bound to Shelterin Core Hub TIN2 when TPP1 Is Absent},
url = {http://www.sciencedirect.com/science/article/pii/S0022283619303225},
volume = {431},
year = {2019}
}
TY - JOUR
ID - 1545278
AU - Janovič, Tomáš - Stojaspal, Martin - Veverka, Pavel - Horáková, Denisa - Hofr, Ctirad
PY - 2019
TI - Human Telomere Repeat Binding Factor TRF1 Replaces TRF2 Bound to Shelterin Core Hub TIN2 when TPP1 Is Absent
JF - Journal of Molecular Biology
VL - 431
IS - 17
SP - 3289-3301
EP - 3289-3301
PB - ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
SN - 00222836
KW - TIN2
KW - telomere
KW - shelterin
KW - assembly
KW - single-molecule
UR - http://www.sciencedirect.com/science/article/pii/S0022283619303225
L2 - http://www.sciencedirect.com/science/article/pii/S0022283619303225
N2 - Human telomeric repeat binding factors TRF1 and TRF2 along with TIN2 form the core of the shelterin complex that protects chromosome ends against unwanted end-joining and DNA repair. We applied a single-molecule approach to assess TRF1–TIN2–TRF2 complex formation in solution at physiological conditions. Fluorescence cross-correlation spectroscopy was used to describe the complex assembly by analyzing how coincident fluctuations of differently labeled TRF1 and TRF2 correlate when they move together through the confocal volume of the microscope. We observed, at the single-molecule level, that TRF1 effectively substitutes TRF2 on TIN2. We assessed also the effect of another telomeric factor TPP1 that recruits telomerase to telomeres. We found that TPP1 upon binding to TIN2 induces changes that expand TIN2 binding capacity, such that TIN2 can accommodate both TRF1 and TRF2 simultaneously. We suggest a molecular model that explains why TPP1 is essential for the stable formation of TRF1–TIN2–TRF2 core complex.
ER -
JANOVIČ, Tomáš, Martin STOJASPAL, Pavel VEVERKA, Denisa HORÁKOVÁ and Ctirad HOFR. Human Telomere Repeat Binding Factor TRF1 Replaces TRF2 Bound to Shelterin Core Hub TIN2 when TPP1 Is Absent. \textit{Journal of Molecular Biology}. London: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2019, vol.~431, No~17, p.~3289-3301. ISSN~0022-2836. Available from: https://dx.doi.org/10.1016/j.jmb.2019.05.038.
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