Neutrophil and Granulocytic Myeloid-Derived Suppressor
Cell-Mediated T Cell Suppression Significantly Contributes to
Immune Dysregulation in Common Variable Immunodeficiency
Disorders

J 2019

Neutrophil and Granulocytic Myeloid-Derived Suppressor Cell-Mediated T Cell Suppression Significantly Contributes to Immune Dysregulation in Common Variable Immunodeficiency Disorders

VLKOVÁ, Marcela, Zita CHOVANCOVÁ, Jana NECHVÁTALOVÁ, Ashley Nicole CONNELLY, Marcus Darrell DAVIS et. al.

Basic information

Original name

Neutrophil and Granulocytic Myeloid-Derived Suppressor Cell-Mediated T Cell Suppression Significantly Contributes to Immune Dysregulation in Common Variable Immunodeficiency Disorders

Authors

VLKOVÁ, Marcela (203 Czech Republic, guarantor, belonging to the institution), Zita CHOVANCOVÁ (203 Czech Republic, belonging to the institution), Jana NECHVÁTALOVÁ (203 Czech Republic, belonging to the institution), Ashley Nicole CONNELLY (840 United States of America), Marcus Darrell DAVIS (840 United States of America), Peter SLANINA (703 Slovakia, belonging to the institution), Lucie KUČEROVÁ (203 Czech Republic, belonging to the institution), Marek LITZMAN (203 Czech Republic), Tereza GRYMOVÁ (203 Czech Republic, belonging to the institution), Přemysl SOUČEK (203 Czech Republic, belonging to the institution), Tomáš FREIBERGER (203 Czech Republic, belonging to the institution), Jiří LITZMAN (203 Czech Republic, belonging to the institution) and Zdenek HEL (203 Czech Republic)

Edition

Journal of immunology, Bethesda, American association of immunologists, 2019, 0022-1767

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30102 Immunology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.886

RIV identification code

RIV/00216224:14110/19:00108489

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.4049/jimmunol.1800102

UT WoS

000454422600011

Keywords in English

Common variable immunodeficiency disorders

Abstract

V originále

Common variable immunodeficiency disorders (CVID) represent a group of primary immunodeficiency diseases characterized by hypogammaglobulinemia and impaired specific Ab response, resulting in recurrent infections due to dysfunctional immune response. The specific mechanisms mediating immune deficiency in CVID remain to be determined. Previous studies indicated that immune dysregulation in CVID patients is associated with chronic microbial translocation, systemic immune activation, and altered homeostasis of lymphocytic and myeloid lineages. A detailed phenotypic, functional characterization of plasma markers and immune cell populations was performed in 46 CVID patients and 44 healthy donors. CVID patients displayed significantly elevated plasma levels of a marker of neutrophil activation neutrophil gelatinase-associated lipocalin. Neutrophils from CVID patients exhibited elevated surface levels of CD11b and PD-L1 and decreased levels of CD62L, CD16, and CD80, consistent with a phenotype of activated neutrophils with suppressive properties. Neutrophils from CVID patients actively suppressed T cell activation and release of IFN-gamma via the production of reactive oxygen species. Furthermore, CVID was associated with an increased frequency of low-density neutrophils (LDNs)/granulocytic myeloid-derived suppressor cells. LDN/granulocytic myeloid-derived suppressor cell frequency in CVID patients correlated with reduced T cell responsiveness. Exogenous stimulation of whole blood with bacterial LPS emulated some but not all of the phenotypic changes observed on neutrophils from CVID patients and induced neutrophil population with LDN phenotype. The presented data demonstrate that neutrophils in the blood of CVID patients acquire an activated phenotype and exert potent T cell suppressive activity. Specific targeting of myeloid cell-derived suppressor activity represents a novel potential therapeutic strategy for CVID.

Links

NV15-28732A, research and development project

Name: Vliv granulocytů a monocytů na vznik a rozvoj imunodeficitních chorob a dalších imunopatologických dějů

Citovat

VLKOVÁ, Marcela, Zita CHOVANCOVÁ, Jana NECHVÁTALOVÁ, Ashley Nicole CONNELLY, Marcus Darrell DAVIS, Peter SLANINA, Lucie KUČEROVÁ, Marek LITZMAN, Tereza GRYMOVÁ, Přemysl SOUČEK, Tomáš FREIBERGER, Jiří LITZMAN and Zdenek HEL. Neutrophil and Granulocytic Myeloid-Derived Suppressor Cell-Mediated T Cell Suppression Significantly Contributes to Immune Dysregulation in Common Variable Immunodeficiency Disorders. Journal of immunology. Bethesda: American association of immunologists, 2019, vol. 202, No 1, p. 93-104. ISSN 0022-1767. Available from: https://dx.doi.org/10.4049/jimmunol.1800102.

@article{1545802,
   author = {Vlková, Marcela and Chovancová, Zita and Nechvátalová, Jana and Connelly, Ashley Nicole and Davis, Marcus Darrell and Slanina, Peter and Kučerová, Lucie and Litzman, Marek and Grymová, Tereza and Souček, Přemysl and Freiberger, Tomáš and Litzman, Jiří and Hel, Zdenek},
   article_location = {Bethesda},
   article_number = {1},
   doi = {http://dx.doi.org/10.4049/jimmunol.1800102},
   keywords = {Common variable immunodeficiency disorders},
   language = {eng},
   issn = {0022-1767},
   journal = {Journal of immunology},
   title = {Neutrophil and Granulocytic Myeloid-Derived Suppressor Cell-Mediated T Cell Suppression Significantly Contributes to Immune Dysregulation in Common Variable Immunodeficiency Disorders},
   url = {https://www.jimmunol.org/content/202/1/93},
   volume = {202},
   year = {2019}
}

TY  - JOUR
ID  - 1545802
AU  - Vlková, Marcela - Chovancová, Zita - Nechvátalová, Jana - Connelly, Ashley Nicole - Davis, Marcus Darrell - Slanina, Peter - Kučerová, Lucie - Litzman, Marek - Grymová, Tereza - Souček, Přemysl - Freiberger, Tomáš - Litzman, Jiří - Hel, Zdenek
PY  - 2019
TI  - Neutrophil and Granulocytic Myeloid-Derived Suppressor Cell-Mediated T Cell Suppression Significantly Contributes to Immune Dysregulation in Common Variable Immunodeficiency Disorders
JF  - Journal of immunology
VL  - 202
IS  - 1
SP  - 93-104
EP  - 93-104
PB  - American association of immunologists
SN  - 00221767
KW  - Common variable immunodeficiency disorders
UR  - https://www.jimmunol.org/content/202/1/93
L2  - https://www.jimmunol.org/content/202/1/93
N2  - Common variable immunodeficiency disorders (CVID) represent a group of primary immunodeficiency diseases characterized by hypogammaglobulinemia and impaired specific Ab response, resulting in recurrent infections due to dysfunctional immune response. The specific mechanisms mediating immune deficiency in CVID remain to be determined. Previous studies indicated that immune dysregulation in CVID patients is associated with chronic microbial translocation, systemic immune activation, and altered homeostasis of lymphocytic and myeloid lineages. A detailed phenotypic, functional characterization of plasma markers and immune cell populations was performed in 46 CVID patients and 44 healthy donors. CVID patients displayed significantly elevated plasma levels of a marker of neutrophil activation neutrophil gelatinase-associated lipocalin. Neutrophils from CVID patients exhibited elevated surface levels of CD11b and PD-L1 and decreased levels of CD62L, CD16, and CD80, consistent with a phenotype of activated neutrophils with suppressive properties. Neutrophils from CVID patients actively suppressed T cell activation and release of IFN-gamma via the production of reactive oxygen species. Furthermore, CVID was associated with an increased frequency of low-density neutrophils (LDNs)/granulocytic myeloid-derived suppressor cells. LDN/granulocytic myeloid-derived suppressor cell frequency in CVID patients correlated with reduced T cell responsiveness. Exogenous stimulation of whole blood with bacterial LPS emulated some but not all of the phenotypic changes observed on neutrophils from CVID patients and induced neutrophil population with LDN phenotype. The presented data demonstrate that neutrophils in the blood of CVID patients acquire an activated phenotype and exert potent T cell suppressive activity. Specific targeting of myeloid cell-derived suppressor activity represents a novel potential therapeutic strategy for CVID.
ER  -

VLKOVÁ, Marcela, Zita CHOVANCOVÁ, Jana NECHVÁTALOVÁ, Ashley Nicole CONNELLY, Marcus Darrell DAVIS, Peter SLANINA, Lucie KUČEROVÁ, Marek LITZMAN, Tereza GRYMOVÁ, Přemysl SOUČEK, Tomáš FREIBERGER, Jiří LITZMAN and Zdenek HEL. Neutrophil and Granulocytic Myeloid-Derived Suppressor Cell-Mediated T Cell Suppression Significantly Contributes to Immune Dysregulation in Common Variable Immunodeficiency Disorders. \textit{Journal of immunology}. Bethesda: American association of immunologists, 2019, vol.~202, No~1, p.~93-104. ISSN~0022-1767. Available from: https://dx.doi.org/10.4049/jimmunol.1800102.

Detailed Information on Publication Record