GRYMOVÁ, Tereza, Marcela VLKOVÁ, Přemysl SOUČEK, Roman HAKL, Jana NECHVÁTALOVÁ, Peter SLANINA, Julie ŠTÍCHOVÁ, Jiří LITZMAN and Tomáš FREIBERGER. Neutrophils Are Dysregulated in Patients with Hereditary Angioedema Types I and II in a Symptom-Free Period. Mediators of Inflammation. New York: Hindawi Publishing Corporation, 2019, vol. 2019, No 9515628, p. 1-12. ISSN 0962-9351. doi:10.1155/2019/9515628.
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Basic information
Original name Neutrophils Are Dysregulated in Patients with Hereditary Angioedema Types I and II in a Symptom-Free Period
Authors GRYMOVÁ, Tereza (203 Czech Republic, belonging to the institution), Marcela VLKOVÁ (203 Czech Republic, belonging to the institution), Přemysl SOUČEK (203 Czech Republic, belonging to the institution), Roman HAKL (203 Czech Republic, belonging to the institution), Jana NECHVÁTALOVÁ (203 Czech Republic, belonging to the institution), Peter SLANINA (703 Slovakia, belonging to the institution), Julie ŠTÍCHOVÁ (203 Czech Republic, belonging to the institution), Jiří LITZMAN (203 Czech Republic, belonging to the institution) and Tomáš FREIBERGER (203 Czech Republic, guarantor, belonging to the institution).
Edition Mediators of Inflammation, New York, Hindawi Publishing Corporation, 2019, 0962-9351.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30102 Immunology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.758
RIV identification code RIV/00216224:14110/19:00108490
Organization unit Faculty of Medicine
UT WoS 000470179800001
Keywords in English Neutrophils
Tags 14110114, podil, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Pavla Foltynová, Ph.D., učo 106624. Changed: 9/3/2020 09:25.
Neutrophils impact on processes preceding the formation of bradykinin, a major swelling mediator in hereditary angioedema (HAE), yet their potential role in HAE pathogenesis has not been sufficiently studied. We assessed the relative mRNA expression of 10 genes related to neutrophil activation using RNA extracted from the peripheral blood neutrophils of 23 HAE patients in a symptom-free period and 39 healthy donors. Increased relative mRNA expression levels of CD274, IL1B, IL1RN, IL8, MMP9, and TLR4, together with a lack in their mutual correlations detected in HAE patients compared to healthy controls, suggested a preactivated state and dysregulation of patients' neutrophils. Patients' neutrophil-alerted state was further supported by increased CD11b, decreased CD16 plasma membrane deposition, and increased relative CD274(+) and CD87(+) neutrophil counts, but not by increased neutrophil elastase or myeloperoxidase plasma levels. As CD274 mediates inhibitory signals to different immune cells, neutrophils were cocultured with T-cells/PBMC. The decrease in CD25(+) and IFN-(+) T-cell/PBMC ratio in patients indicated the patients' neutrophil suppressive functions. In summary, the results showed neutrophils' alerted state and dysregulation at the transcript level in patients with HAE types I and II even in a symptom-free period, which might make them more susceptible to edema formation. Neutrophils' T-cell suppressive capacity in HAE patients needs to be further investigated.
MUNI/A/0925/2017, internal MU codeName: Poruchy tvorby protilátek a komplementového systému
Investor: Masaryk University, Grant Agency of Masaryk University, Category A
NV15-28732A, research and development projectName: Vliv granulocytů a monocytů na vznik a rozvoj imunodeficitních chorob a dalších imunopatologických dějů
NV18-05-00330, research and development projectName: Genetická determinace závažnosti otoků podmíněných bradykininem u pacientů s hereditárním angioedémem
Investor: Ministry of Health of the CR, Ministry of Health Research Programme 2015 - 2022
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