Neutrophils Are Dysregulated in Patients with Hereditary
Angioedema Types I and II in a Symptom-Free Period

J 2019

Neutrophils Are Dysregulated in Patients with Hereditary Angioedema Types I and II in a Symptom-Free Period

GRYMOVÁ, Tereza, Marcela VLKOVÁ, Přemysl SOUČEK, Roman HAKL, Jana NECHVÁTALOVÁ et. al.

Basic information

Original name

Neutrophils Are Dysregulated in Patients with Hereditary Angioedema Types I and II in a Symptom-Free Period

Authors

GRYMOVÁ, Tereza (203 Czech Republic, belonging to the institution), Marcela VLKOVÁ (203 Czech Republic, belonging to the institution), Přemysl SOUČEK (203 Czech Republic, belonging to the institution), Roman HAKL (203 Czech Republic, belonging to the institution), Jana NECHVÁTALOVÁ (203 Czech Republic, belonging to the institution), Peter SLANINA (703 Slovakia, belonging to the institution), Julie ŠTÍCHOVÁ (203 Czech Republic, belonging to the institution), Jiří LITZMAN (203 Czech Republic, belonging to the institution) and Tomáš FREIBERGER (203 Czech Republic, guarantor, belonging to the institution)

Edition

Mediators of Inflammation, New York, Hindawi Publishing Corporation, 2019, 0962-9351

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30102 Immunology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.758

RIV identification code

RIV/00216224:14110/19:00108490

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1155/2019/9515628

UT WoS

000470179800001

Keywords in English

Neutrophils

Abstract

V originále

Neutrophils impact on processes preceding the formation of bradykinin, a major swelling mediator in hereditary angioedema (HAE), yet their potential role in HAE pathogenesis has not been sufficiently studied. We assessed the relative mRNA expression of 10 genes related to neutrophil activation using RNA extracted from the peripheral blood neutrophils of 23 HAE patients in a symptom-free period and 39 healthy donors. Increased relative mRNA expression levels of CD274, IL1B, IL1RN, IL8, MMP9, and TLR4, together with a lack in their mutual correlations detected in HAE patients compared to healthy controls, suggested a preactivated state and dysregulation of patients' neutrophils. Patients' neutrophil-alerted state was further supported by increased CD11b, decreased CD16 plasma membrane deposition, and increased relative CD274(+) and CD87(+) neutrophil counts, but not by increased neutrophil elastase or myeloperoxidase plasma levels. As CD274 mediates inhibitory signals to different immune cells, neutrophils were cocultured with T-cells/PBMC. The decrease in CD25(+) and IFN-(+) T-cell/PBMC ratio in patients indicated the patients' neutrophil suppressive functions. In summary, the results showed neutrophils' alerted state and dysregulation at the transcript level in patients with HAE types I and II even in a symptom-free period, which might make them more susceptible to edema formation. Neutrophils' T-cell suppressive capacity in HAE patients needs to be further investigated.

Links

MUNI/A/0925/2017, interní kód MU

Name: Poruchy tvorby protilátek a komplementového systému

Investor: Masaryk University, Category A

NV15-28732A, research and development project

Name: Vliv granulocytů a monocytů na vznik a rozvoj imunodeficitních chorob a dalších imunopatologických dějů

NV18-05-00330, research and development project

Name: Genetická determinace závažnosti otoků podmíněných bradykininem u pacientů s hereditárním angioedémem

Investor: Ministry of Health of the CR, Genetic determination of bradykinin-mediated angioedema severity in patients with hereditary angioedema

Citovat

GRYMOVÁ, Tereza, Marcela VLKOVÁ, Přemysl SOUČEK, Roman HAKL, Jana NECHVÁTALOVÁ, Peter SLANINA, Julie ŠTÍCHOVÁ, Jiří LITZMAN and Tomáš FREIBERGER. Neutrophils Are Dysregulated in Patients with Hereditary Angioedema Types I and II in a Symptom-Free Period. Mediators of Inflammation. New York: Hindawi Publishing Corporation, 2019, vol. 2019, No 9515628, p. 1-12. ISSN 0962-9351. Available from: https://dx.doi.org/10.1155/2019/9515628.

@article{1545803,
   author = {Grymová, Tereza and Vlková, Marcela and Souček, Přemysl and Hakl, Roman and Nechvátalová, Jana and Slanina, Peter and Štíchová, Julie and Litzman, Jiří and Freiberger, Tomáš},
   article_location = {New York},
   article_number = {9515628},
   doi = {http://dx.doi.org/10.1155/2019/9515628},
   keywords = {Neutrophils},
   language = {eng},
   issn = {0962-9351},
   journal = {Mediators of Inflammation},
   title = {Neutrophils Are Dysregulated in Patients with Hereditary Angioedema Types I and II in a Symptom-Free Period},
   url = {https://www.hindawi.com/journals/mi/2019/9515628/},
   volume = {2019},
   year = {2019}
}

TY  - JOUR
ID  - 1545803
AU  - Grymová, Tereza - Vlková, Marcela - Souček, Přemysl - Hakl, Roman - Nechvátalová, Jana - Slanina, Peter - Štíchová, Julie - Litzman, Jiří - Freiberger, Tomáš
PY  - 2019
TI  - Neutrophils Are Dysregulated in Patients with Hereditary Angioedema Types I and II in a Symptom-Free Period
JF  - Mediators of Inflammation
VL  - 2019
IS  - 9515628
SP  - 1-12
EP  - 1-12
PB  - Hindawi Publishing Corporation
SN  - 09629351
KW  - Neutrophils
UR  - https://www.hindawi.com/journals/mi/2019/9515628/
L2  - https://www.hindawi.com/journals/mi/2019/9515628/
N2  - Neutrophils impact on processes preceding the formation of bradykinin, a major swelling mediator in hereditary angioedema (HAE), yet their potential role in HAE pathogenesis has not been sufficiently studied. We assessed the relative mRNA expression of 10 genes related to neutrophil activation using RNA extracted from the peripheral blood neutrophils of 23 HAE patients in a symptom-free period and 39 healthy donors. Increased relative mRNA expression levels of CD274, IL1B, IL1RN, IL8, MMP9, and TLR4, together with a lack in their mutual correlations detected in HAE patients compared to healthy controls, suggested a preactivated state and dysregulation of patients' neutrophils. Patients' neutrophil-alerted state was further supported by increased CD11b, decreased CD16 plasma membrane deposition, and increased relative CD274(+) and CD87(+) neutrophil counts, but not by increased neutrophil elastase or myeloperoxidase plasma levels. As CD274 mediates inhibitory signals to different immune cells, neutrophils were cocultured with T-cells/PBMC. The decrease in CD25(+) and IFN-(+) T-cell/PBMC ratio in patients indicated the patients' neutrophil suppressive functions. In summary, the results showed neutrophils' alerted state and dysregulation at the transcript level in patients with HAE types I and II even in a symptom-free period, which might make them more susceptible to edema formation. Neutrophils' T-cell suppressive capacity in HAE patients needs to be further investigated.
ER  -

GRYMOVÁ, Tereza, Marcela VLKOVÁ, Přemysl SOUČEK, Roman HAKL, Jana NECHVÁTALOVÁ, Peter SLANINA, Julie ŠTÍCHOVÁ, Jiří LITZMAN and Tomáš FREIBERGER. Neutrophils Are Dysregulated in Patients with Hereditary Angioedema Types I and II in a Symptom-Free Period. \textit{Mediators of Inflammation}. New York: Hindawi Publishing Corporation, 2019, vol.~2019, No~9515628, p.~1-12. ISSN~0962-9351. Available from: https://dx.doi.org/10.1155/2019/9515628.

Detailed Information on Publication Record