Current vascular allograft procurement, cryopreservation and
transplantation techniques in the Czech Republic

J 2019

Current vascular allograft procurement, cryopreservation and transplantation techniques in the Czech Republic

ŠPAČEK, Miroslav, Pavel MĚŘIČKA, Libor JANOUŠEK, Petr ŠTÁDLER, Miloš ADAMEC et. al.

Basic information

Original name

Current vascular allograft procurement, cryopreservation and transplantation techniques in the Czech Republic

Authors

ŠPAČEK, Miroslav (203 Czech Republic, guarantor), Pavel MĚŘIČKA (203 Czech Republic), Libor JANOUŠEK (203 Czech Republic), Petr ŠTÁDLER (203 Czech Republic), Miloš ADAMEC (203 Czech Republic), Robert VLACHOVSKÝ (203 Czech Republic, belonging to the institution), Igor GUŇKA (203 Czech Republic), Pavel NAVRÁTIL (203 Czech Republic), Filip THIEME (203 Czech Republic), Rudolf ŠPUNDA (203 Czech Republic), Jan BURKERT (203 Czech Republic), Robert STAFFA (203 Czech Republic, belonging to the institution), Petr NĚMEC (203 Czech Republic) and Jaroslav LINDNER (203 Czech Republic)

Edition

ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE, WROCLAW, WROCLAW MEDICAL UNIV, 2019, 1899-5276

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30212 Surgery

Country of publisher

Poland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.514

RIV identification code

RIV/00216224:14110/19:00110189

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.17219/acem/90037

UT WoS

000466338100012

Keywords in English

tissue banking; cryopreserved vascular allograft transplantation; operative procedures; graft procurement; cryopreservation

Abstract

V originále

Background. Vascular allotransplantations are performed worldwide in selected patients suffering from vascular prosthesis infection or critical limb ischemia. Either fresh or cryopreserved vascular allograft may be used. Objectives. In various points, we address several aspects (allograft procurement, cryopreservation and transplantation technique) of the program of vascular allotransplantations in the Czech Republic. Materials and methods. Vascular grafts retrieval has been done within multiorgan harvests using no-touch technique. Very short time of cold ischemia is achieved due to close cooperation with Tissue Establishment where the following processing of cryopreservation is performed. Meeting all necessary quality criteria is a prerequisity for releasing grafts for clinical application. Standardized thawing protocol and surgical handling aims to minimize microfractures before implantation. Results. Based on experimental and clinical work, the first validation of cryopreserved arterial and venous grafts for clinical use was performed between 2011 and 2013 in the Czech Republic. The developement of storage of vascular tissue in banks was stimulated in 2000-2010 by the issue of EU directives and national harmonized norms, aimed at assurance of high quality and safety of cells and tissues used for transplantations in humans. Conclusions. There are several crucial moments affecting final quality, including graft retrieval within a multiorgan harvest, short ischemic time, cryopreservation, and thawing technique used. The recommended surgical handling during implantation may also affect results and graft-related complications.

Citovat

ŠPAČEK, Miroslav, Pavel MĚŘIČKA, Libor JANOUŠEK, Petr ŠTÁDLER, Miloš ADAMEC, Robert VLACHOVSKÝ, Igor GUŇKA, Pavel NAVRÁTIL, Filip THIEME, Rudolf ŠPUNDA, Jan BURKERT, Robert STAFFA, Petr NĚMEC and Jaroslav LINDNER. Current vascular allograft procurement, cryopreservation and transplantation techniques in the Czech Republic. ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE. WROCLAW: WROCLAW MEDICAL UNIV, 2019, vol. 28, No 4, p. 529-534. ISSN 1899-5276. Available from: https://dx.doi.org/10.17219/acem/90037.

@article{1545958,
   author = {Špaček, Miroslav and Měřička, Pavel and Janoušek, Libor and Štádler, Petr and Adamec, Miloš and Vlachovský, Robert and Guňka, Igor and Navrátil, Pavel and Thieme, Filip and Špunda, Rudolf and Burkert, Jan and Staffa, Robert and Němec, Petr and Lindner, Jaroslav},
   article_location = {WROCLAW},
   article_number = {4},
   doi = {http://dx.doi.org/10.17219/acem/90037},
   keywords = {tissue banking; cryopreserved vascular allograft transplantation; operative procedures; graft procurement; cryopreservation},
   language = {eng},
   issn = {1899-5276},
   journal = {ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE},
   title = {Current vascular allograft procurement, cryopreservation and transplantation techniques in the Czech Republic},
   url = {http://dx.doi.org/10.17219/acem/90037},
   volume = {28},
   year = {2019}
}

TY  - JOUR
ID  - 1545958
AU  - Špaček, Miroslav - Měřička, Pavel - Janoušek, Libor - Štádler, Petr - Adamec, Miloš - Vlachovský, Robert - Guňka, Igor - Navrátil, Pavel - Thieme, Filip - Špunda, Rudolf - Burkert, Jan - Staffa, Robert - Němec, Petr - Lindner, Jaroslav
PY  - 2019
TI  - Current vascular allograft procurement, cryopreservation and transplantation techniques in the Czech Republic
JF  - ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE
VL  - 28
IS  - 4
SP  - 529-534
EP  - 529-534
PB  - WROCLAW MEDICAL UNIV
SN  - 18995276
KW  - tissue banking
KW  - cryopreserved vascular allograft transplantation
KW  - operative procedures
KW  - graft procurement
KW  - cryopreservation
UR  - http://dx.doi.org/10.17219/acem/90037
L2  - http://dx.doi.org/10.17219/acem/90037
N2  - Background. Vascular allotransplantations are performed worldwide in selected patients suffering from vascular prosthesis infection or critical limb ischemia. Either fresh or cryopreserved vascular allograft may be used. Objectives. In various points, we address several aspects (allograft procurement, cryopreservation and transplantation technique) of the program of vascular allotransplantations in the Czech Republic. Materials and methods. Vascular grafts retrieval has been done within multiorgan harvests using no-touch technique. Very short time of cold ischemia is achieved due to close cooperation with Tissue Establishment where the following processing of cryopreservation is performed. Meeting all necessary quality criteria is a prerequisity for releasing grafts for clinical application. Standardized thawing protocol and surgical handling aims to minimize microfractures before implantation. Results. Based on experimental and clinical work, the first validation of cryopreserved arterial and venous grafts for clinical use was performed between 2011 and 2013 in the Czech Republic. The developement of storage of vascular tissue in banks was stimulated in 2000-2010 by the issue of EU directives and national harmonized norms, aimed at assurance of high quality and safety of cells and tissues used for transplantations in humans. Conclusions. There are several crucial moments affecting final quality, including graft retrieval within a multiorgan harvest, short ischemic time, cryopreservation, and thawing technique used. The recommended surgical handling during implantation may also affect results and graft-related complications.
ER  -

ŠPAČEK, Miroslav, Pavel MĚŘIČKA, Libor JANOUŠEK, Petr ŠTÁDLER, Miloš ADAMEC, Robert VLACHOVSKÝ, Igor GUŇKA, Pavel NAVRÁTIL, Filip THIEME, Rudolf ŠPUNDA, Jan BURKERT, Robert STAFFA, Petr NĚMEC and Jaroslav LINDNER. Current vascular allograft procurement, cryopreservation and transplantation techniques in the Czech Republic. \textit{ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE}. WROCLAW: WROCLAW MEDICAL UNIV, 2019, vol.~28, No~4, p.~529-534. ISSN~1899-5276. Available from: https://dx.doi.org/10.17219/acem/90037.

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