| ŠPAČEK, Miroslav, Pavel MĚŘIČKA, Libor JANOUŠEK, Petr ŠTÁDLER, Miloš ADAMEC, Robert VLACHOVSKÝ, Igor GUŇKA, Pavel NAVRÁTIL, Filip THIEME, Rudolf ŠPUNDA, Jan BURKERT, Robert STAFFA, Petr NĚMEC and Jaroslav LINDNER. Current vascular allograft procurement, cryopreservation and transplantation techniques in the Czech Republic. ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE. WROCLAW: WROCLAW MEDICAL UNIV, 2019, vol. 28, No 4, p. 529-534. ISSN 1899-5276. Available from: https://dx.doi.org/10.17219/acem/90037. |
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@article{1545958,
author = {Špaček, Miroslav and Měřička, Pavel and Janoušek, Libor and Štádler, Petr and Adamec, Miloš and Vlachovský, Robert and Guňka, Igor and Navrátil, Pavel and Thieme, Filip and Špunda, Rudolf and Burkert, Jan and Staffa, Robert and Němec, Petr and Lindner, Jaroslav},
article_location = {WROCLAW},
article_number = {4},
doi = {http://dx.doi.org/10.17219/acem/90037},
keywords = {tissue banking; cryopreserved vascular allograft transplantation; operative procedures; graft procurement; cryopreservation},
language = {eng},
issn = {1899-5276},
journal = {ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE},
title = {Current vascular allograft procurement, cryopreservation and transplantation techniques in the Czech Republic},
url = {http://dx.doi.org/10.17219/acem/90037},
volume = {28},
year = {2019}
}
TY - JOUR
ID - 1545958
AU - Špaček, Miroslav - Měřička, Pavel - Janoušek, Libor - Štádler, Petr - Adamec, Miloš - Vlachovský, Robert - Guňka, Igor - Navrátil, Pavel - Thieme, Filip - Špunda, Rudolf - Burkert, Jan - Staffa, Robert - Němec, Petr - Lindner, Jaroslav
PY - 2019
TI - Current vascular allograft procurement, cryopreservation and transplantation techniques in the Czech Republic
JF - ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE
VL - 28
IS - 4
SP - 529-534
EP - 529-534
PB - WROCLAW MEDICAL UNIV
SN - 18995276
KW - tissue banking
KW - cryopreserved vascular allograft transplantation
KW - operative procedures
KW - graft procurement
KW - cryopreservation
UR - http://dx.doi.org/10.17219/acem/90037
L2 - http://dx.doi.org/10.17219/acem/90037
N2 - Background. Vascular allotransplantations are performed worldwide in selected patients suffering from vascular prosthesis infection or critical limb ischemia. Either fresh or cryopreserved vascular allograft may be used. Objectives. In various points, we address several aspects (allograft procurement, cryopreservation and transplantation technique) of the program of vascular allotransplantations in the Czech Republic. Materials and methods. Vascular grafts retrieval has been done within multiorgan harvests using no-touch technique. Very short time of cold ischemia is achieved due to close cooperation with Tissue Establishment where the following processing of cryopreservation is performed. Meeting all necessary quality criteria is a prerequisity for releasing grafts for clinical application. Standardized thawing protocol and surgical handling aims to minimize microfractures before implantation. Results. Based on experimental and clinical work, the first validation of cryopreserved arterial and venous grafts for clinical use was performed between 2011 and 2013 in the Czech Republic. The developement of storage of vascular tissue in banks was stimulated in 2000-2010 by the issue of EU directives and national harmonized norms, aimed at assurance of high quality and safety of cells and tissues used for transplantations in humans. Conclusions. There are several crucial moments affecting final quality, including graft retrieval within a multiorgan harvest, short ischemic time, cryopreservation, and thawing technique used. The recommended surgical handling during implantation may also affect results and graft-related complications.
ER -
ŠPAČEK, Miroslav, Pavel MĚŘIČKA, Libor JANOUŠEK, Petr ŠTÁDLER, Miloš ADAMEC, Robert VLACHOVSKÝ, Igor GUŇKA, Pavel NAVRÁTIL, Filip THIEME, Rudolf ŠPUNDA, Jan BURKERT, Robert STAFFA, Petr NĚMEC and Jaroslav LINDNER. Current vascular allograft procurement, cryopreservation and transplantation techniques in the Czech Republic. \textit{ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE}. WROCLAW: WROCLAW MEDICAL UNIV, 2019, vol.~28, No~4, p.~529-534. ISSN~1899-5276. Available from: https://dx.doi.org/10.17219/acem/90037.
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