Multicomponent Yeast Two-Hybrid System: Applications to Study
Protein-Protein Interactions in SMC Complexes.

C 2019

Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes.

PALEČEK, Jan, Lucie VONDROVÁ, Kateřina ZÁBRADY and Jakub OTOČKA

Basic information

Original name

Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes.

Authors

PALEČEK, Jan (203 Czech Republic, guarantor, belonging to the institution), Lucie VONDROVÁ (203 Czech Republic, belonging to the institution), Kateřina ZÁBRADY (203 Czech Republic) and Jakub OTOČKA (703 Slovakia, belonging to the institution)

Edition

New York, NY, Methods in Molecular Biology, p. 79-90, 12 pp. SMC Complexes, vol 2004. 2019

Publisher

Springer Nature

Other information

Language

English

Type of outcome

Kapitola resp. kapitoly v odborné knize

Field of Study

10609 Biochemical research methods

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

printed version "print"

References:

URL

RIV identification code

RIV/00216224:14310/19:00107533

Organization unit

Faculty of Science

ISBN

978-1-4939-9519-6

DOI

http://dx.doi.org/10.1007/978-1-4939-9520-2_7

UT WoS

000487933300008

Keywords in English

KITE proteins; Kleisin bridge; Multicomponent yeast two-hybrid (Y2H) system; Non-SMC Element (Nse) subunits; Protein–protein interactions; SMC5/6; Structural maintenance of chromosomes (SMC) complexes

Abstract

V originále

Analysis of protein-protein interactions (PPI) is key for the understanding of most protein assemblies including structural maintenance of chromosomes (SMC) complexes. SMC complexes are composed of SMC proteins, kleisin, and kleisin-interacting subunits. These subunits interact in specific ways to constitute and regulate the closed structure of the complexes. Specifically, kleisin molecules bridge the SMC dimers and the kleisin-interacting subunits modulate stability of the bridge. Here we describe a multicomponent version of a yeast two-hybrid (Y2H) method and its application for analysis of the bridging role of the Nse4 kleisin in the SMC5/6 complex. Using this technique, we also show a stabilizing effect of KITE (kleisin-interacting tandem winged-helix element) proteins on SMC5/6.

Links

GA18-02067S, research and development project

Name: Úloha Nse1/Nse3/Nse4, E3-ubikvitin ligásy, ve stabilitě genomu

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

PALEČEK, Jan, Lucie VONDROVÁ, Kateřina ZÁBRADY and Jakub OTOČKA. Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes. In Badrinarayanan A. (eds). Methods in Molecular Biology. New York, NY: Springer Nature, 2019, p. 79-90. SMC Complexes, vol 2004. ISBN 978-1-4939-9519-6. Available from: https://dx.doi.org/10.1007/978-1-4939-9520-2_7.

@inbook{1548183,
   author = {Paleček, Jan and Vondrová, Lucie and Zábrady, Kateřina and Otočka, Jakub},
   address = {New York, NY},
   booktitle = {Methods in Molecular Biology},
   doi = {http://dx.doi.org/10.1007/978-1-4939-9520-2_7},
   editor = {Badrinarayanan A. (eds)},
   keywords = {KITE proteins; Kleisin bridge; Multicomponent yeast two-hybrid (Y2H) system; Non-SMC Element (Nse) subunits; Protein–protein interactions; SMC5/6; Structural maintenance of chromosomes (SMC) complexes},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {New York, NY},
   isbn = {978-1-4939-9519-6},
   pages = {79-90},
   publisher = {Springer Nature},
   title = {Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes.},
   url = {https://link.springer.com/protocol/10.1007%2F978-1-4939-9520-2_7},
   year = {2019}
}

TY  - CHAP
ID  - 1548183
AU  - Paleček, Jan - Vondrová, Lucie - Zábrady, Kateřina - Otočka, Jakub
PY  - 2019
TI  - Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes.
VL  - SMC Complexes, vol 2004.
PB  - Springer Nature
CY  - New York, NY
SN  - 9781493995196
KW  - KITE proteins
KW  - Kleisin bridge
KW  - Multicomponent yeast two-hybrid (Y2H) system
KW  - Non-SMC Element (Nse) subunits
KW  - Protein–protein interactions
KW  - SMC5/6
KW  - Structural maintenance of chromosomes (SMC) complexes
UR  - https://link.springer.com/protocol/10.1007%2F978-1-4939-9520-2_7
L2  - https://link.springer.com/protocol/10.1007%2F978-1-4939-9520-2_7
N2  - Analysis of protein-protein interactions (PPI) is key for the understanding of most protein assemblies including structural maintenance of chromosomes (SMC) complexes. SMC complexes are composed of SMC proteins, kleisin, and kleisin-interacting subunits. These subunits interact in specific ways to constitute and regulate the closed structure of the complexes. Specifically, kleisin molecules bridge the SMC dimers and the kleisin-interacting subunits modulate stability of the bridge. Here we describe a multicomponent version of a yeast two-hybrid (Y2H) method and its application for analysis of the bridging role of the Nse4 kleisin in the SMC5/6 complex. Using this technique, we also show a stabilizing effect of KITE (kleisin-interacting tandem winged-helix element) proteins on SMC5/6.
ER  -

PALEČEK, Jan, Lucie VONDROVÁ, Kateřina ZÁBRADY and Jakub OTOČKA. Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes. In Badrinarayanan A. (eds). \textit{Methods in Molecular Biology}. New York, NY: Springer Nature, 2019, p.~79-90. SMC Complexes, vol 2004. ISBN~978-1-4939-9519-6. Available from: https://dx.doi.org/10.1007/978-1-4939-9520-2\_{}7.

Detailed Information on Publication Record