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@inbook{1548183,
author = {Paleček, Jan and Vondrová, Lucie and Zábrady, Kateřina and Otočka, Jakub},
address = {New York, NY},
booktitle = {Methods in Molecular Biology},
doi = {http://dx.doi.org/10.1007/978-1-4939-9520-2_7},
editor = {Badrinarayanan A. (eds)},
keywords = {KITE proteins; Kleisin bridge; Multicomponent yeast two-hybrid (Y2H) system; Non-SMC Element (Nse) subunits; Protein–protein interactions; SMC5/6; Structural maintenance of chromosomes (SMC) complexes},
howpublished = {tištěná verze "print"},
language = {eng},
location = {New York, NY},
isbn = {978-1-4939-9519-6},
pages = {79-90},
publisher = {Springer Nature},
title = {Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes.},
url = {https://link.springer.com/protocol/10.1007%2F978-1-4939-9520-2_7},
year = {2019}
}
TY - CHAP
ID - 1548183
AU - Paleček, Jan - Vondrová, Lucie - Zábrady, Kateřina - Otočka, Jakub
PY - 2019
TI - Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes.
VL - SMC Complexes, vol 2004.
PB - Springer Nature
CY - New York, NY
SN - 9781493995196
KW - KITE proteins
KW - Kleisin bridge
KW - Multicomponent yeast two-hybrid (Y2H) system
KW - Non-SMC Element (Nse) subunits
KW - Protein–protein interactions
KW - SMC5/6
KW - Structural maintenance of chromosomes (SMC) complexes
UR - https://link.springer.com/protocol/10.1007%2F978-1-4939-9520-2_7
L2 - https://link.springer.com/protocol/10.1007%2F978-1-4939-9520-2_7
N2 - Analysis of protein-protein interactions (PPI) is key for the understanding of most protein assemblies including structural maintenance of chromosomes (SMC) complexes. SMC complexes are composed of SMC proteins, kleisin, and kleisin-interacting subunits. These subunits interact in specific ways to constitute and regulate the closed structure of the complexes. Specifically, kleisin molecules bridge the SMC dimers and the kleisin-interacting subunits modulate stability of the bridge. Here we describe a multicomponent version of a yeast two-hybrid (Y2H) method and its application for analysis of the bridging role of the Nse4 kleisin in the SMC5/6 complex. Using this technique, we also show a stabilizing effect of KITE (kleisin-interacting tandem winged-helix element) proteins on SMC5/6.
ER -
PALEČEK, Jan, Lucie VONDROVÁ, Kateřina ZÁBRADY and Jakub OTOČKA. Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes. In Badrinarayanan A. (eds). \textit{Methods in Molecular Biology}. New York, NY: Springer Nature. p.~79-90. SMC Complexes, vol 2004. ISBN~978-1-4939-9519-6. doi:10.1007/978-1-4939-9520-2\_{}7. 2019.
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