Detailed Information on Publication Record
2019
Photorhabdus lectins disrupt the activity of insect and human immune system
DOBEŠ, Pavel, Eva FUJDIAROVÁ, Josef HOUSER, Gita JANČAŘÍKOVÁ, Pavel HYRŠL et. al.Basic information
Original name
Photorhabdus lectins disrupt the activity of insect and human immune system
Authors
DOBEŠ, Pavel (203 Czech Republic, belonging to the institution), Eva FUJDIAROVÁ (203 Czech Republic, belonging to the institution), Josef HOUSER (203 Czech Republic, belonging to the institution), Gita JANČAŘÍKOVÁ (203 Czech Republic, belonging to the institution), Pavel HYRŠL (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
International Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group “Microbial and Nematode Control of Invertebrate Pests” 2019
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10608 Biochemistry and molecular biology
Country of publisher
Spain
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/19:00107542
Organization unit
Faculty of Science
Keywords (in Czech)
Photorhabdus; lektin; imunita; melanizace; kyslíkové radikály
Keywords in English
Photorhabdus; lectin; immunity; melanisation; reactive oxygen species
Tags
International impact
Změněno: 6/8/2019 15:45, Mgr. Pavel Dobeš, Ph.D.
V originále
Bacteria of the genus Photorhabdus are known as potent entomopathogens that produce a variety of toxins, proteases and other virulence factors to overcome host immune system and successfully establish the infection. Although lectins with their carbohydrate-binding abilities are not the typical example of molecules causing direct damage to host organism, they are indispensable in the processes such as attachment to cells, immunoevasion and immunosuppression. Bacteria of the genus Photorhabdus are not an exception as they produce lectins that could help them to interact with nematode symbionts, other bacteria or host immune system. Recently, we focused on lectins produced by P. laumondii (formerly classified as P. luminescens subsp. laumondii) that are not only able to bind to insect haemocytes, but also to disturb cellular and humoral immune response. Lectin treatment of insect haemolymph induced melanisation catalysed by phenoloxidase; this increase was not observed when we used the lectin pre-treated with saccharides selected according to its specificity. Although, P. laumondii is not considered to be human pathogen (unlike closely related P. asymbiotica), its lectin is able to inhibit production of reactive oxygen species in human blood induced by neutrophil activator zymosan A, whereas it is not able to supress the action of other activators such as phorbol 12-myristate 13-acetate or fMLF. Our results suggest that the lectin interferes with Toll-like receptor 2 and thus can impair the production of reactive oxygen species by host phagocytes. The work was supported by the Czech Science Foundation (grants no. 17-03253S and 18-18964S).
In Czech
Bacteria of the genus Photorhabdus are known as potent entomopathogens that produce a variety of toxins, proteases and other virulence factors to overcome host immune system and successfully establish the infection. Although lectins with their carbohydrate-binding abilities are not the typical example of molecules causing direct damage to host organism, they are indispensable in the processes such as attachment to cells, immunoevasion and immunosuppression. Bacteria of the genus Photorhabdus are not an exception as they produce lectins that could help them to interact with nematode symbionts, other bacteria or host immune system. Recently, we focused on lectins produced by P. laumondii (formerly classified as P. luminescens subsp. laumondii) that are not only able to bind to insect haemocytes, but also to disturb cellular and humoral immune response. Lectin treatment of insect haemolymph induced melanisation catalysed by phenoloxidase; this increase was not observed when we used the lectin pre-treated with saccharides selected according to its specificity. Although, P. laumondii is not considered to be human pathogen (unlike closely related P. asymbiotica), its lectin is able to inhibit production of reactive oxygen species in human blood induced by neutrophil activator zymosan A, whereas it is not able to supress the action of other activators such as phorbol 12-myristate 13-acetate or fMLF. Our results suggest that the lectin interferes with Toll-like receptor 2 and thus can impair the production of reactive oxygen species by host phagocytes. The work was supported by the Czech Science Foundation (grants no. 17-03253S and 18-18964S).
Links
| GA17-03253S, research and development project |
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| GA18-18964S, research and development project |
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