DOBEŠ, Pavel, Eva FUJDIAROVÁ, Josef HOUSER, Gita JANČAŘÍKOVÁ, Pavel HYRŠL and Michaela WIMMEROVÁ. Photorhabdus lectins disrupt the activity of insect and human immune system. In International Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group “Microbial and Nematode Control of Invertebrate Pests”. 2019.
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Basic information
Original name Photorhabdus lectins disrupt the activity of insect and human immune system
Authors DOBEŠ, Pavel (203 Czech Republic, belonging to the institution), Eva FUJDIAROVÁ (203 Czech Republic, belonging to the institution), Josef HOUSER (203 Czech Republic, belonging to the institution), Gita JANČAŘÍKOVÁ (203 Czech Republic, belonging to the institution), Pavel HYRŠL (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution).
Edition International Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group “Microbial and Nematode Control of Invertebrate Pests” 2019.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10608 Biochemistry and molecular biology
Country of publisher Spain
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/19:00107542
Organization unit Faculty of Science
Keywords (in Czech) Photorhabdus; lektin; imunita; melanizace; kyslíkové radikály
Keywords in English Photorhabdus; lectin; immunity; melanisation; reactive oxygen species
Tags International impact
Changed by Changed by: Mgr. Pavel Dobeš, Ph.D., učo 150960. Changed: 6/8/2019 15:45.
Abstract
Bacteria of the genus Photorhabdus are known as potent entomopathogens that produce a variety of toxins, proteases and other virulence factors to overcome host immune system and successfully establish the infection. Although lectins with their carbohydrate-binding abilities are not the typical example of molecules causing direct damage to host organism, they are indispensable in the processes such as attachment to cells, immunoevasion and immunosuppression. Bacteria of the genus Photorhabdus are not an exception as they produce lectins that could help them to interact with nematode symbionts, other bacteria or host immune system. Recently, we focused on lectins produced by P. laumondii (formerly classified as P. luminescens subsp. laumondii) that are not only able to bind to insect haemocytes, but also to disturb cellular and humoral immune response. Lectin treatment of insect haemolymph induced melanisation catalysed by phenoloxidase; this increase was not observed when we used the lectin pre-treated with saccharides selected according to its specificity. Although, P. laumondii is not considered to be human pathogen (unlike closely related P. asymbiotica), its lectin is able to inhibit production of reactive oxygen species in human blood induced by neutrophil activator zymosan A, whereas it is not able to supress the action of other activators such as phorbol 12-myristate 13-acetate or fMLF. Our results suggest that the lectin interferes with Toll-like receptor 2 and thus can impair the production of reactive oxygen species by host phagocytes. The work was supported by the Czech Science Foundation (grants no. 17-03253S and 18-18964S).
Abstract (in Czech)
Bacteria of the genus Photorhabdus are known as potent entomopathogens that produce a variety of toxins, proteases and other virulence factors to overcome host immune system and successfully establish the infection. Although lectins with their carbohydrate-binding abilities are not the typical example of molecules causing direct damage to host organism, they are indispensable in the processes such as attachment to cells, immunoevasion and immunosuppression. Bacteria of the genus Photorhabdus are not an exception as they produce lectins that could help them to interact with nematode symbionts, other bacteria or host immune system. Recently, we focused on lectins produced by P. laumondii (formerly classified as P. luminescens subsp. laumondii) that are not only able to bind to insect haemocytes, but also to disturb cellular and humoral immune response. Lectin treatment of insect haemolymph induced melanisation catalysed by phenoloxidase; this increase was not observed when we used the lectin pre-treated with saccharides selected according to its specificity. Although, P. laumondii is not considered to be human pathogen (unlike closely related P. asymbiotica), its lectin is able to inhibit production of reactive oxygen species in human blood induced by neutrophil activator zymosan A, whereas it is not able to supress the action of other activators such as phorbol 12-myristate 13-acetate or fMLF. Our results suggest that the lectin interferes with Toll-like receptor 2 and thus can impair the production of reactive oxygen species by host phagocytes. The work was supported by the Czech Science Foundation (grants no. 17-03253S and 18-18964S).
Links
GA17-03253S, research and development projectName: Hormonální kontrola hmyzího obranného systému
Investor: Czech Science Foundation
GA18-18964S, research and development projectName: Lektiny a jejich úloha v interakci patogen/hostitel a buněčném rozpoznávání
Investor: Czech Science Foundation
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