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@article{1548610,
author = {Vreman, Hendrik J. and Kourula, Stephanie and Jasprova, Jana and Ludvíková, Lucie and Klán, Petr and Muchova, Lucie and Vitek, Libor and Cline, Benjamin K. and Wong, Ronald J. and Stevenson, David K.},
article_location = {Rotterdam},
article_number = {6},
doi = {http://dx.doi.org/10.1038/s41390-019-0310-2},
keywords = {HUMAN-SERUM ALBUMIN; QUANTUM YIELD; STRUCTURAL PHOTOISOMERIZATION; PHOTOTHERAPY; ISOMERIZATION; DEPENDENCE; ISOMER; TURQUOISE; INFANTS; HYPERBILIRUBINEMIA},
language = {eng},
issn = {0031-3998},
journal = {Pediatric Research},
title = {The effect of light wavelength on in vitro bilirubin photodegradation and photoisomer production},
url = {https://www.nature.com/articles/s41390-019-0310-2},
volume = {85},
year = {2019}
}
TY - JOUR
ID - 1548610
AU - Vreman, Hendrik J. - Kourula, Stephanie - Jasprova, Jana - Ludvíková, Lucie - Klán, Petr - Muchova, Lucie - Vitek, Libor - Cline, Benjamin K. - Wong, Ronald J. - Stevenson, David K.
PY - 2019
TI - The effect of light wavelength on in vitro bilirubin photodegradation and photoisomer production
JF - Pediatric Research
VL - 85
IS - 6
SP - 865-873
EP - 865-873
PB - Williams and Wilkins
SN - 00313998
KW - HUMAN-SERUM ALBUMIN
KW - QUANTUM YIELD
KW - STRUCTURAL PHOTOISOMERIZATION
KW - PHOTOTHERAPY
KW - ISOMERIZATION
KW - DEPENDENCE
KW - ISOMER
KW - TURQUOISE
KW - INFANTS
KW - HYPERBILIRUBINEMIA
UR - https://www.nature.com/articles/s41390-019-0310-2
L2 - https://www.nature.com/articles/s41390-019-0310-2
N2 - BACKGROUND: The action spectrum for bilirubin photodegradation has been intensively studied. However, questions still remain regarding which light wavelength most efficiently photodegrades bilirubin. In this study, we determined the in vitro effects of different irradiation wavelength ranges on bilirubin photodegradation. METHODS: In our in vitro method, normalized absolute irradiance levels of 4.2 x 10(15) photons/cm(2)/s from light-emitting diodes (ranging from 390-530 nm) and 10-nm band-pass filters were used to irradiate bilirubin solutions (25 mg/dL in 4% human serum albumin). Bilirubin and its major photoisomer concentrations were determined; the half-life time of bilirubin (t(1/2)) was calculated for each wavelength range, and the spectral characteristics for bilirubin photodegradation products were obtained for key wavelengths. RESULTS: The in vitro photodegradation of bilirubin at 37 degrees C decreased linearly as the wavelength was increased from 390 to 500 nm with t(1/2) decreasing from 63 to 17 min, respectively. At 460 +/- 10 nm, a significantly lower rate of photodegradation and thus higher t(1/2) (31 min) than that at 500 nm (17 min) was demonstrated. CONCLUSION: In our system, the optimum bilirubin photodegradation and lumirubin production rates occurred between 490 and 500 nm. Spectra shapes were remarkably similar, suggesting that lumirubin production was the major process of bilirubin photodegradation.
ER -
VREMAN, Hendrik J., Stephanie KOURULA, Jana JASPROVA, Lucie LUDVÍKOVÁ, Petr KLÁN, Lucie MUCHOVA, Libor VITEK, Benjamin K. CLINE, Ronald J. WONG and David K. STEVENSON. The effect of light wavelength on in vitro bilirubin photodegradation and photoisomer production. \textit{Pediatric Research}. Rotterdam: Williams and Wilkins, 2019, vol.~85, No~6, p.~865-873. ISSN~0031-3998. Available from: https://dx.doi.org/10.1038/s41390-019-0310-2.
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