Immunomodulatory effects of cyanobacterial toxin
cylindrospermopsin on innate immune cells

J 2019

Immunomodulatory effects of cyanobacterial toxin cylindrospermopsin on innate immune cells

MOOSOVÁ, Zdena, Michaela PEKAROVÁ, Lenka ŠINDLEROVÁ, Ondřej VAŠÍČEK, Lukáš KUBALA et. al.

Basic information

Original name

Immunomodulatory effects of cyanobacterial toxin cylindrospermopsin on innate immune cells

Authors

MOOSOVÁ, Zdena (203 Czech Republic, belonging to the institution), Michaela PEKAROVÁ (703 Slovakia), Lenka ŠINDLEROVÁ (203 Czech Republic, belonging to the institution), Ondřej VAŠÍČEK (203 Czech Republic), Lukáš KUBALA (203 Czech Republic), Luděk BLÁHA (203 Czech Republic, guarantor, belonging to the institution) and Ondřej ADAMOVSKÝ (203 Czech Republic, belonging to the institution)

Edition

Chemosphere, Oxford, PERGAMON-ELSEVIER SCIENCE LTD, 2019, 0045-6535

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10511 Environmental sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.778

RIV identification code

RIV/00216224:14310/19:00107557

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.chemosphere.2019.03.143

UT WoS

000468709500048

Keywords in English

Cylindrospermopsin; Cyanobacteria; Immunity; Inflammation; Macrophages; LPS

Abstract

V originále

Cylindrospermopsin (CYN), a cyanobacterial toxin, is an important water pollutant with broad biological activity. It has been known mainly from tropical areas, but the area of occurrence of its producers is spreading to temperate climates. It can be found in high concentrations in the environment as well as in purified drinking waters. The aim of the study is to bring a basic information on the ability of CYN to interfere with mammalian innate immunity cells and thus increase the understanding of the immunomodulatory potency of CYN. This study investigated whether immune cells can be a target of CYN either alone or in combination with a model immunomodulatory agent, lipopolysaccharide (LPS). We examined the effects on cellular viability and inflammation signaling of CYN on murine macrophage-like RAW 264.7 cells. Macrophages were treated either with pure toxin (1 mu M) or together with a known stimulator of immunologically active cells, bacterial or cyanobacterial LPS. CYN has had a significant effect on production on pro-inflammatory mediator tumor necrosis factor alpha (TNF-alpha) which correlates with its effect on reactive oxygen species (ROS) production. We found that CYN potentiated the effect of bacterial and cyanobacterial LPS that was documented by activation of inflammatory signaling pathways including mitogen-activated protein kinase p38 as well as consequent expression of inducible nitric oxide synthase (iNOS) and increased production of pro-inflammatory mediators such as nitric oxide (NO), TNF-alpha, interleukin-6 (IL-6). Our study brings one of the first information that contributes to the elucidation of immunomodulatory role of CYN in macrophages under normal and pro-inflammatory conditions.

Links

EF16_013/0001761, research and development project

Name: RECETOX RI

GA16-24949S, research and development project

Name: Význam Toll-like receptorů v odpovědi střevních epiteliálních buněk na cyanobakterie vodních květů

Investor: Czech Science Foundation

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

MOOSOVÁ, Zdena, Michaela PEKAROVÁ, Lenka ŠINDLEROVÁ, Ondřej VAŠÍČEK, Lukáš KUBALA, Luděk BLÁHA and Ondřej ADAMOVSKÝ. Immunomodulatory effects of cyanobacterial toxin cylindrospermopsin on innate immune cells. Chemosphere. Oxford: PERGAMON-ELSEVIER SCIENCE LTD, 2019, vol. 226, July, p. 439-446. ISSN 0045-6535. Available from: https://dx.doi.org/10.1016/j.chemosphere.2019.03.143.

@article{1548997,
   author = {Moosová, Zdena and Pekarová, Michaela and Šindlerová, Lenka and Vašíček, Ondřej and Kubala, Lukáš and Bláha, Luděk and Adamovský, Ondřej},
   article_location = {Oxford},
   article_number = {July},
   doi = {http://dx.doi.org/10.1016/j.chemosphere.2019.03.143},
   keywords = {Cylindrospermopsin; Cyanobacteria; Immunity; Inflammation; Macrophages; LPS},
   language = {eng},
   issn = {0045-6535},
   journal = {Chemosphere},
   title = {Immunomodulatory effects of cyanobacterial toxin cylindrospermopsin on innate immune cells},
   url = {https://www.sciencedirect.com/science/article/pii/S0045653519305892?via%3Dihub},
   volume = {226},
   year = {2019}
}

TY  - JOUR
ID  - 1548997
AU  - Moosová, Zdena - Pekarová, Michaela - Šindlerová, Lenka - Vašíček, Ondřej - Kubala, Lukáš - Bláha, Luděk - Adamovský, Ondřej
PY  - 2019
TI  - Immunomodulatory effects of cyanobacterial toxin cylindrospermopsin on innate immune cells
JF  - Chemosphere
VL  - 226
IS  - July
SP  - 439-446
EP  - 439-446
PB  - PERGAMON-ELSEVIER SCIENCE LTD
SN  - 00456535
KW  - Cylindrospermopsin
KW  - Cyanobacteria
KW  - Immunity
KW  - Inflammation
KW  - Macrophages
KW  - LPS
UR  - https://www.sciencedirect.com/science/article/pii/S0045653519305892?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0045653519305892?via%3Dihub
N2  - Cylindrospermopsin (CYN), a cyanobacterial toxin, is an important water pollutant with broad biological activity. It has been known mainly from tropical areas, but the area of occurrence of its producers is spreading to temperate climates. It can be found in high concentrations in the environment as well as in purified drinking waters. The aim of the study is to bring a basic information on the ability of CYN to interfere with mammalian innate immunity cells and thus increase the understanding of the immunomodulatory potency of CYN. This study investigated whether immune cells can be a target of CYN either alone or in combination with a model immunomodulatory agent, lipopolysaccharide (LPS). We examined the effects on cellular viability and inflammation signaling of CYN on murine macrophage-like RAW 264.7 cells. Macrophages were treated either with pure toxin (1 mu M) or together with a known stimulator of immunologically active cells, bacterial or cyanobacterial LPS. CYN has had a significant effect on production on pro-inflammatory mediator tumor necrosis factor alpha (TNF-alpha) which correlates with its effect on reactive oxygen species (ROS) production. We found that CYN potentiated the effect of bacterial and cyanobacterial LPS that was documented by activation of inflammatory signaling pathways including mitogen-activated protein kinase p38 as well as consequent expression of inducible nitric oxide synthase (iNOS) and increased production of pro-inflammatory mediators such as nitric oxide (NO), TNF-alpha, interleukin-6 (IL-6). Our study brings one of the first information that contributes to the elucidation of immunomodulatory role of CYN in macrophages under normal and pro-inflammatory conditions.
ER  -

MOOSOVÁ, Zdena, Michaela PEKAROVÁ, Lenka ŠINDLEROVÁ, Ondřej VAŠÍČEK, Lukáš KUBALA, Luděk BLÁHA and Ondřej ADAMOVSKÝ. Immunomodulatory effects of cyanobacterial toxin cylindrospermopsin on innate immune cells. \textit{Chemosphere}. Oxford: PERGAMON-ELSEVIER SCIENCE LTD, 2019, vol.~226, July, p.~439-446. ISSN~0045-6535. Available from: https://dx.doi.org/10.1016/j.chemosphere.2019.03.143.

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