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@article{1548997,
author = {Moosová, Zdena and Pekarová, Michaela and Šindlerová, Lenka and Vašíček, Ondřej and Kubala, Lukáš and Bláha, Luděk and Adamovský, Ondřej},
article_location = {Oxford},
article_number = {July},
doi = {http://dx.doi.org/10.1016/j.chemosphere.2019.03.143},
keywords = {Cylindrospermopsin; Cyanobacteria; Immunity; Inflammation; Macrophages; LPS},
language = {eng},
issn = {0045-6535},
journal = {Chemosphere},
title = {Immunomodulatory effects of cyanobacterial toxin cylindrospermopsin on innate immune cells},
url = {https://www.sciencedirect.com/science/article/pii/S0045653519305892?via%3Dihub},
volume = {226},
year = {2019}
}
TY - JOUR
ID - 1548997
AU - Moosová, Zdena - Pekarová, Michaela - Šindlerová, Lenka - Vašíček, Ondřej - Kubala, Lukáš - Bláha, Luděk - Adamovský, Ondřej
PY - 2019
TI - Immunomodulatory effects of cyanobacterial toxin cylindrospermopsin on innate immune cells
JF - Chemosphere
VL - 226
IS - July
SP - 439-446
EP - 439-446
PB - PERGAMON-ELSEVIER SCIENCE LTD
SN - 00456535
KW - Cylindrospermopsin
KW - Cyanobacteria
KW - Immunity
KW - Inflammation
KW - Macrophages
KW - LPS
UR - https://www.sciencedirect.com/science/article/pii/S0045653519305892?via%3Dihub
L2 - https://www.sciencedirect.com/science/article/pii/S0045653519305892?via%3Dihub
N2 - Cylindrospermopsin (CYN), a cyanobacterial toxin, is an important water pollutant with broad biological activity. It has been known mainly from tropical areas, but the area of occurrence of its producers is spreading to temperate climates. It can be found in high concentrations in the environment as well as in purified drinking waters. The aim of the study is to bring a basic information on the ability of CYN to interfere with mammalian innate immunity cells and thus increase the understanding of the immunomodulatory potency of CYN. This study investigated whether immune cells can be a target of CYN either alone or in combination with a model immunomodulatory agent, lipopolysaccharide (LPS). We examined the effects on cellular viability and inflammation signaling of CYN on murine macrophage-like RAW 264.7 cells. Macrophages were treated either with pure toxin (1 mu M) or together with a known stimulator of immunologically active cells, bacterial or cyanobacterial LPS. CYN has had a significant effect on production on pro-inflammatory mediator tumor necrosis factor alpha (TNF-alpha) which correlates with its effect on reactive oxygen species (ROS) production. We found that CYN potentiated the effect of bacterial and cyanobacterial LPS that was documented by activation of inflammatory signaling pathways including mitogen-activated protein kinase p38 as well as consequent expression of inducible nitric oxide synthase (iNOS) and increased production of pro-inflammatory mediators such as nitric oxide (NO), TNF-alpha, interleukin-6 (IL-6). Our study brings one of the first information that contributes to the elucidation of immunomodulatory role of CYN in macrophages under normal and pro-inflammatory conditions.
ER -
MOOSOVÁ, Zdena, Michaela PEKAROVÁ, Lenka ŠINDLEROVÁ, Ondřej VAŠÍČEK, Lukáš KUBALA, Luděk BLÁHA and Ondřej ADAMOVSKÝ. Immunomodulatory effects of cyanobacterial toxin cylindrospermopsin on innate immune cells. \textit{Chemosphere}. Oxford: PERGAMON-ELSEVIER SCIENCE LTD, 2019, vol.~226, July, p.~439-446. ISSN~0045-6535. Available from: https://dx.doi.org/10.1016/j.chemosphere.2019.03.143.
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