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@article{1549458, author = {Svobodníková, Lucie and Kummerová, Marie and Zezulka, Štěpán and Babula, Petr}, article_location = {SAN DIEGO}, article_number = {6}, doi = {http://dx.doi.org/10.1016/j.ecoenv.2019.109369}, keywords = {Tobacco BY-2; Diclofenac; Cell viability; Oxidative stress; Mitotic index; Apoptosis}, language = {eng}, issn = {0147-6513}, journal = {Ecotoxicology and Environmental Safety}, title = {Possible use of a Nicotiana tabacum ‘Bright Yellow 2’ cell suspension as a model to assess phytotoxicity of pharmaceuticals (diclofenac)}, url = {https://www.sciencedirect.com/science/article/pii/S0147651319306906?via%3Dihub}, volume = {182}, year = {2019} }
TY - JOUR ID - 1549458 AU - Svobodníková, Lucie - Kummerová, Marie - Zezulka, Štěpán - Babula, Petr PY - 2019 TI - Possible use of a Nicotiana tabacum ‘Bright Yellow 2’ cell suspension as a model to assess phytotoxicity of pharmaceuticals (diclofenac) JF - Ecotoxicology and Environmental Safety VL - 182 IS - 6 SP - 1-10 EP - 1-10 PB - ACADEMIC PRESS INC ELSEVIER SCIENCE SN - 01476513 KW - Tobacco BY-2 KW - Diclofenac KW - Cell viability KW - Oxidative stress KW - Mitotic index KW - Apoptosis UR - https://www.sciencedirect.com/science/article/pii/S0147651319306906?via%3Dihub L2 - https://www.sciencedirect.com/science/article/pii/S0147651319306906?via%3Dihub N2 - Growth and developmental changes in plants induced by pharmaceuticals reflect changes in processes at the cellular and subcellular levels. Due to their growth and cellular characteristics, plant cell suspension cultures can be a suitable model for assessing toxicity. In this study, 10–1000 ug/L of the non-steroidal anti-inflammatory drug diclofenac (DCF) decreased the viability of Nicotiana tabacum BY-2 cells after 24 h of treatment. Further, 0.1–10 mg/L DCF diminished the density of the cell suspension by 9–46% after 96 h of treatment, but at 1 and 10 ug/L, DCF increased the density by 13% and 5%, respectively, after 120 h. These changes were accompanied by increased production of total reactive oxygen species (ROS) and mitochondrial superoxide (up to 17-fold and 5-fold, respectively), and a decrease in the mitochondrial membrane potential (by ~64%) especially at 1000 ug/L DCF. The increased ROS production was accompanied by decrease in level of reactive nitrogen species (RNS; by 36%) and total thiols (by 61%). Damage to BY-2 cells was evidenced by accumulation of neutral red in acidic compartments (up to 10-fold at 1000 ug/L DCF), and increase of autophagic vacuole formation (up to 8-fold at 1000 ug/L DCF). Furthermore, irregular or stretched nuclei were observed in nearly 27% and 50% of cells at 100 and 1000 ug/L DCF, respectively. Highest levels of chromatin condensation (11% of cells) and apoptotic DNA fragmentation (7%) were found at 10 ug/L DCF. The results revealed a significant effect of DCF on BY-2 cells after 24 h of exposure. Changes in the growth and viability parameters were indisputably related to ROS and RNS production, changes in mitochondrial function, and possible activation of processes leading to cell death. ER -
SVOBODNÍKOVÁ, Lucie, Marie KUMMEROVÁ, Štěpán ZEZULKA and Petr BABULA. Possible use of a Nicotiana tabacum ‘Bright Yellow 2’ cell suspension as a model to assess phytotoxicity of pharmaceuticals (diclofenac). \textit{Ecotoxicology and Environmental Safety}. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2019, vol.~182, No~6, p.~1-10. ISSN~0147-6513. Available from: https://dx.doi.org/10.1016/j.ecoenv.2019.109369.
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