Lipopolysaccharides from Microcystis Cyanobacteria-Dominated
Water Bloom and from Laboratory Cultures Trigger Human Immune
Innate Response

J 2019

Lipopolysaccharides from Microcystis Cyanobacteria-Dominated Water Bloom and from Laboratory Cultures Trigger Human Immune Innate Response

MOOSOVÁ, Zdena, Lenka ŠINDLEROVÁ, Barbora AMBRUZOVA, Gabriela AMBROŽOVÁ, Ondřej VAŠÍČEK et. al.

Basic information

Original name

Lipopolysaccharides from Microcystis Cyanobacteria-Dominated Water Bloom and from Laboratory Cultures Trigger Human Immune Innate Response

Authors

MOOSOVÁ, Zdena (203 Czech Republic, belonging to the institution), Lenka ŠINDLEROVÁ (203 Czech Republic, belonging to the institution), Barbora AMBRUZOVA (203 Czech Republic), Gabriela AMBROŽOVÁ (203 Czech Republic), Ondřej VAŠÍČEK (203 Czech Republic, belonging to the institution), Mirna VELKI (191 Croatia), Pavel BABICA (203 Czech Republic, belonging to the institution) and Lukáš KUBALA (203 Czech Republic, guarantor, belonging to the institution)

Edition

TOXINS, BASEL, MDPI, 2019, 2072-6651

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

40401 Agricultural biotechnology and food biotechnology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Full Text

Impact factor

Impact factor: 3.531

RIV identification code

RIV/00216224:14310/19:00107573

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/toxins11040218

UT WoS

000467299600036

Keywords in English

water bloom; cyanobacteria; endotoxin; lipopolysaccharide; inflammation; leukocytes

Abstract

V originále

Massive toxic blooms of cyanobacteria represent a major threat to water supplies worldwide. Here, the biological activities of lipopolysaccharide (LPS) isolated from Microcystis aeruginosa, the most prominent cyanobacteria in water bloom, were studied. LPS was isolated from complex environmental water bloom samples dominated by M. aeruginosa, and from laboratory cultures of non-axenic as well as axenic M. aeruginosa strains PCC7806 and HAMBI/UHCC130. Employing human blood-based in vitro tests, the LPS isolated from complex water bloom revealed the priming of both major blood phagocyte population monocytes and polymorphonuclear leukocytes documented by the increased surface expression of CD11b and CD66b. This was accompanied by a water bloom LPS-mediated dose-dependent induction of tumor necrosis factor , interleukin-1, and interleukin-6 production. In accordance with its priming effects, water bloom LPS induced significant activation of p38 and ERK1/2 kinases, as well as NF-B phosphorylation, in isolated polymorphonuclear leukocytes. Interestingly, the pro-inflammatory potential of LPS from the axenic strain of M. aeruginosa was not lower compared to that of LPS isolated from non-axenic strains. In contrast to the biological activity, water bloom LPS revealed almost twice higher pyrogenicity levels compared to Escherichia coli LPS, as analyzed by the PyroGene test. Moreover, LPS from the non-axenic culture exhibited higher endotoxin activity in comparison to LPS from axenic strains. Taking the above findings together, M. aeruginosa LPS can contribute to the health risks associated with contamination by complex water bloom mass.

Links

EF16_013/0001761, research and development project

Name: RECETOX RI

GA16-24949S, research and development project

Name: Význam Toll-like receptorů v odpovědi střevních epiteliálních buněk na cyanobakterie vodních květů

Investor: Czech Science Foundation

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

MOOSOVÁ, Zdena, Lenka ŠINDLEROVÁ, Barbora AMBRUZOVA, Gabriela AMBROŽOVÁ, Ondřej VAŠÍČEK, Mirna VELKI, Pavel BABICA and Lukáš KUBALA. Lipopolysaccharides from Microcystis Cyanobacteria-Dominated Water Bloom and from Laboratory Cultures Trigger Human Immune Innate Response. TOXINS. BASEL: MDPI, 2019, vol. 11, No 4, p. 241-251. ISSN 2072-6651. Available from: https://dx.doi.org/10.3390/toxins11040218.

@article{1549503,
   author = {Moosová, Zdena and Šindlerová, Lenka and Ambruzova, Barbora and Ambrožová, Gabriela and Vašíček, Ondřej and Velki, Mirna and Babica, Pavel and Kubala, Lukáš},
   article_location = {BASEL},
   article_number = {4},
   doi = {http://dx.doi.org/10.3390/toxins11040218},
   keywords = {water bloom; cyanobacteria; endotoxin; lipopolysaccharide; inflammation; leukocytes},
   language = {eng},
   issn = {2072-6651},
   journal = {TOXINS},
   title = {Lipopolysaccharides from Microcystis Cyanobacteria-Dominated Water Bloom and from Laboratory Cultures Trigger Human Immune Innate Response},
   url = {https://www.mdpi.com/2072-6651/11/4/218},
   volume = {11},
   year = {2019}
}

TY  - JOUR
ID  - 1549503
AU  - Moosová, Zdena - Šindlerová, Lenka - Ambruzova, Barbora - Ambrožová, Gabriela - Vašíček, Ondřej - Velki, Mirna - Babica, Pavel - Kubala, Lukáš
PY  - 2019
TI  - Lipopolysaccharides from Microcystis Cyanobacteria-Dominated Water Bloom and from Laboratory Cultures Trigger Human Immune Innate Response
JF  - TOXINS
VL  - 11
IS  - 4
SP  - 241-251
EP  - 241-251
PB  - MDPI
SN  - 20726651
KW  - water bloom
KW  - cyanobacteria
KW  - endotoxin
KW  - lipopolysaccharide
KW  - inflammation
KW  - leukocytes
UR  - https://www.mdpi.com/2072-6651/11/4/218
L2  - https://www.mdpi.com/2072-6651/11/4/218
N2  - Massive toxic blooms of cyanobacteria represent a major threat to water supplies worldwide. Here, the biological activities of lipopolysaccharide (LPS) isolated from Microcystis aeruginosa, the most prominent cyanobacteria in water bloom, were studied. LPS was isolated from complex environmental water bloom samples dominated by M. aeruginosa, and from laboratory cultures of non-axenic as well as axenic M. aeruginosa strains PCC7806 and HAMBI/UHCC130. Employing human blood-based in vitro tests, the LPS isolated from complex water bloom revealed the priming of both major blood phagocyte population monocytes and polymorphonuclear leukocytes documented by the increased surface expression of CD11b and CD66b. This was accompanied by a water bloom LPS-mediated dose-dependent induction of tumor necrosis factor , interleukin-1, and interleukin-6 production. In accordance with its priming effects, water bloom LPS induced significant activation of p38 and ERK1/2 kinases, as well as NF-B phosphorylation, in isolated polymorphonuclear leukocytes. Interestingly, the pro-inflammatory potential of LPS from the axenic strain of M. aeruginosa was not lower compared to that of LPS isolated from non-axenic strains. In contrast to the biological activity, water bloom LPS revealed almost twice higher pyrogenicity levels compared to Escherichia coli LPS, as analyzed by the PyroGene test. Moreover, LPS from the non-axenic culture exhibited higher endotoxin activity in comparison to LPS from axenic strains. Taking the above findings together, M. aeruginosa LPS can contribute to the health risks associated with contamination by complex water bloom mass.
ER  -

MOOSOVÁ, Zdena, Lenka ŠINDLEROVÁ, Barbora AMBRUZOVA, Gabriela AMBROŽOVÁ, Ondřej VAŠÍČEK, Mirna VELKI, Pavel BABICA and Lukáš KUBALA. Lipopolysaccharides from Microcystis Cyanobacteria-Dominated Water Bloom and from Laboratory Cultures Trigger Human Immune Innate Response. \textit{TOXINS}. BASEL: MDPI, 2019, vol.~11, No~4, p.~241-251. ISSN~2072-6651. Available from: https://dx.doi.org/10.3390/toxins11040218.

Detailed Information on Publication Record