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@article{1549658,
author = {Eduardo, Mariana Bustamante and Popovici, Vlad and Imboden, Sara and Aebi, Stefan and Ballabio, Nadja and Altermatt, Hans Jorg and Gunthert, Andreas and Jaggi, Rolf},
article_location = {London},
article_number = {June},
doi = {http://dx.doi.org/10.1186/s12885-019-5752-8},
keywords = {Breast cancer; Molecular risk scores; Local recurrence; Brain metastasis; PAM50 subtypes; Gene expression measurement; Immunohistochemistry; RNA isolation and processing; Hierarchical clustering},
language = {eng},
issn = {1471-2407},
journal = {BMC Cancer},
title = {Characterization of molecular scores and gene expression signatures in primary breast cancer, local recurrences and brain metastases},
url = {https://bmccancer.biomedcentral.com/articles/10.1186/s12885-019-5752-8},
volume = {19},
year = {2019}
}
TY - JOUR
ID - 1549658
AU - Eduardo, Mariana Bustamante - Popovici, Vlad - Imboden, Sara - Aebi, Stefan - Ballabio, Nadja - Altermatt, Hans Jorg - Gunthert, Andreas - Jaggi, Rolf
PY - 2019
TI - Characterization of molecular scores and gene expression signatures in primary breast cancer, local recurrences and brain metastases
JF - BMC Cancer
VL - 19
IS - June
SP - 1-14
EP - 1-14
PB - BioMed Central
SN - 14712407
KW - Breast cancer
KW - Molecular risk scores
KW - Local recurrence
KW - Brain metastasis
KW - PAM50 subtypes
KW - Gene expression measurement
KW - Immunohistochemistry
KW - RNA isolation and processing
KW - Hierarchical clustering
UR - https://bmccancer.biomedcentral.com/articles/10.1186/s12885-019-5752-8
L2 - https://bmccancer.biomedcentral.com/articles/10.1186/s12885-019-5752-8
N2 - BackgroundBreast cancer is a leading cause of cancer-related death in women worldwide. Despite extensive studies in all areas of basic, clinical and applied research, accurate prognosis remains elusive, thus leading to overtreatment of many patients. Diagnosis could be improved by introducing multigene molecular scores in standard clinical practice. Several tests that work with formalin-fixed tissue have become routine. Molecular scores usually include several genes representing processes, response to oestrogens, progestogens and human epidermal growth factor receptor 2 (Her2), respectively, which are combined additively in single values. These multi-gene scores have the advantage of being more robust and reproducible than single-gene scores. Their utility may be further enhanced by combining them with classical diagnostic parameters. Here, we present an exploratory study comparing the RISK and research versions of Oncotype DX recurrence score (RS), Prosigna Risk of Recurrence (ROR) and EndoPredict (EP) with respect to their prognostic potential for ipsilateral recurrence and/or distant relapse in brain, and we compared the scores to the intrinsic subtypes based on PAM50.MethodsRNA was extracted from formalin-fixed, paraffin-embedded (FFPE) tissue cores of primary tumours, local recurrences and brain metastases. Gene expression was measured on a NanoString nCounter Analysis System. Intrinsic subtypes and molecular scores were computed according to published literature and RISK, RS, ROR and EP were compared against each other and to the intrinsic subtypes Luminal A (lumA), Luminal B (lumB), Her2-enriched (Her2), Basal-like (basal), and Normal-like (normal) of PAM50. Local recurrences and brain metastases were compared to their corresponding primary tumours.ResultsAll four molecular scores were highly correlated. Highest correlations were observed among genes related to proliferation while lower correlations were found among oestrogen-related genes. The scores were significantly higher in primary tumours progressing to brain metastases as compared to recurrence-free primary tumours and primary tumours that relapsed as local recurrences.ConclusionsRISK and ROR-P are prognostic for primary tumours metastasizing to the brain. All four scores, RISK, RS, EP and ROR-P failed to discriminate between primary tumours that remained recurrence-free and primary tumours relapsing as local recurrences.
ER -
EDUARDO, Mariana Bustamante, Vlad POPOVICI, Sara IMBODEN, Stefan AEBI, Nadja BALLABIO, Hans Jorg ALTERMATT, Andreas GUNTHERT and Rolf JAGGI. Characterization of molecular scores and gene expression signatures in primary breast cancer, local recurrences and brain metastases. \textit{BMC Cancer}. London: BioMed Central, 2019, vol.~19, June, p.~1-14. ISSN~1471-2407. Available from: https://dx.doi.org/10.1186/s12885-019-5752-8.
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