MODLITBOVÁ, Pavlína, Zdeněk FARKA, Matěj PASTUCHA, Pavel POŘÍZKA, Karel NOVOTNÝ, Petr SKLÁDAL and Jozef KAISER. Laser-induced breakdown spectroscopy as a novel readout method for nanoparticle-based immunoassays. Microchimica Acta. Vienna: Springer, 2019, vol. 186, No 9, p. 1-10. ISSN 0026-3672. Available from: https://dx.doi.org/10.1007/s00604-019-3742-9. |
Other formats:
BibTeX
LaTeX
RIS
@article{1549721, author = {Modlitbová, Pavlína and Farka, Zdeněk and Pastucha, Matěj and Pořízka, Pavel and Novotný, Karel and Skládal, Petr and Kaiser, Jozef}, article_location = {Vienna}, article_number = {9}, doi = {http://dx.doi.org/10.1007/s00604-019-3742-9}, keywords = {Collinear plasma collection; Gold nanoparticles; Laser ablation; Microtiter plate; Sandwich immunoassay; Silver nanoparticles; Streptavidin; Tag-LIBS}, language = {eng}, issn = {0026-3672}, journal = {Microchimica Acta}, title = {Laser-induced breakdown spectroscopy as a novel readout method for nanoparticle-based immunoassays}, url = {https://link.springer.com/article/10.1007%2Fs00604-019-3742-9}, volume = {186}, year = {2019} }
TY - JOUR ID - 1549721 AU - Modlitbová, Pavlína - Farka, Zdeněk - Pastucha, Matěj - Pořízka, Pavel - Novotný, Karel - Skládal, Petr - Kaiser, Jozef PY - 2019 TI - Laser-induced breakdown spectroscopy as a novel readout method for nanoparticle-based immunoassays JF - Microchimica Acta VL - 186 IS - 9 SP - 1-10 EP - 1-10 PB - Springer SN - 00263672 KW - Collinear plasma collection KW - Gold nanoparticles KW - Laser ablation KW - Microtiter plate KW - Sandwich immunoassay KW - Silver nanoparticles KW - Streptavidin KW - Tag-LIBS UR - https://link.springer.com/article/10.1007%2Fs00604-019-3742-9 L2 - https://link.springer.com/article/10.1007%2Fs00604-019-3742-9 N2 - Laser-induced breakdown spectroscopy (LIBS) was examined as a novel method for readout of microtiter plate immunoassays involving nanoparticles (NP). The so-called Tag-LIBS technique is a sensitive method for the detection of specific biomarkers. It was applied to the determination of NP labels using nanosecond ablation sampling. The NP labels were examined from the bottom of a standard 96-well microtiter plate. Thanks to the flexibility of LIBS instrumentation, both the plasma emission collection and the focusing optics arrangements can be collinearly arranged. The experiments showed that silver NPs and gold NPs can be readily quantified on the bottom of the microtiter plate. Utilizing this technique, a sandwich immunoassay for human serum albumin using streptavidin-coated AgNP labels was developed. The assay has a 10 ng·mL-1 detection limit which is comparable to the sensitivity of fluorometric readout. The main advantage of this LIBS technique is its wide scope in which it enables a detection of almost any type of NP labels, irrespective to any fluorescence or catalytic properties. Owing to the immediate signal response, the relatively simple instrumentation also enables assay automation. The LIBS capability of multi-elemental analyses makes it a promising and fast alternative to other readout techniques, in particular with respect to multiplexed detection of biomarkers. ER -
MODLITBOVÁ, Pavlína, Zdeněk FARKA, Matěj PASTUCHA, Pavel POŘÍZKA, Karel NOVOTNÝ, Petr SKLÁDAL and Jozef KAISER. Laser-induced breakdown spectroscopy as a novel readout method for nanoparticle-based immunoassays. \textit{Microchimica Acta}. Vienna: Springer, 2019, vol.~186, No~9, p.~1-10. ISSN~0026-3672. Available from: https://dx.doi.org/10.1007/s00604-019-3742-9.
|