Laser-induced breakdown spectroscopy as a novel readout method
for nanoparticle-based immunoassays

J 2019

Laser-induced breakdown spectroscopy as a novel readout method for nanoparticle-based immunoassays

MODLITBOVÁ, Pavlína, Zdeněk FARKA, Matěj PASTUCHA, Pavel POŘÍZKA, Karel NOVOTNÝ et. al.

Basic information

Original name

Laser-induced breakdown spectroscopy as a novel readout method for nanoparticle-based immunoassays

Authors

MODLITBOVÁ, Pavlína (203 Czech Republic), Zdeněk FARKA (203 Czech Republic, belonging to the institution), Matěj PASTUCHA (203 Czech Republic, belonging to the institution), Pavel POŘÍZKA (203 Czech Republic), Karel NOVOTNÝ (203 Czech Republic, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Jozef KAISER (203 Czech Republic, guarantor)

Edition

Microchimica Acta, Vienna, Springer, 2019, 0026-3672

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Austria

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.232

RIV identification code

RIV/00216224:14740/19:00110447

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1007/s00604-019-3742-9

UT WoS

000481742100001

Keywords in English

Collinear plasma collection; Gold nanoparticles; Laser ablation; Microtiter plate; Sandwich immunoassay; Silver nanoparticles; Streptavidin; Tag-LIBS

Abstract

V originále

Laser-induced breakdown spectroscopy (LIBS) was examined as a novel method for readout of microtiter plate immunoassays involving nanoparticles (NP). The so-called Tag-LIBS technique is a sensitive method for the detection of specific biomarkers. It was applied to the determination of NP labels using nanosecond ablation sampling. The NP labels were examined from the bottom of a standard 96-well microtiter plate. Thanks to the flexibility of LIBS instrumentation, both the plasma emission collection and the focusing optics arrangements can be collinearly arranged. The experiments showed that silver NPs and gold NPs can be readily quantified on the bottom of the microtiter plate. Utilizing this technique, a sandwich immunoassay for human serum albumin using streptavidin-coated AgNP labels was developed. The assay has a 10 ng·mL-1 detection limit which is comparable to the sensitivity of fluorometric readout. The main advantage of this LIBS technique is its wide scope in which it enables a detection of almost any type of NP labels, irrespective to any fluorescence or catalytic properties. Owing to the immediate signal response, the relatively simple instrumentation also enables assay automation. The LIBS capability of multi-elemental analyses makes it a promising and fast alternative to other readout techniques, in particular with respect to multiplexed detection of biomarkers.

Links

CZ.02.1.01/0.0/0.0/16_013/0001728, interní kód MU

Name: CEITEC Nano+ (Acronym: CEITEC Nano)

Investor: Ministry of Education, Youth and Sports of the CR, CEITEC Nano+ (CEITEC Nano), Priority axis 1: Strengthening capacities for high-quality research

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

MODLITBOVÁ, Pavlína, Zdeněk FARKA, Matěj PASTUCHA, Pavel POŘÍZKA, Karel NOVOTNÝ, Petr SKLÁDAL and Jozef KAISER. Laser-induced breakdown spectroscopy as a novel readout method for nanoparticle-based immunoassays. Microchimica Acta. Vienna: Springer, 2019, vol. 186, No 9, p. 1-10. ISSN 0026-3672. Available from: https://dx.doi.org/10.1007/s00604-019-3742-9.

@article{1549721,
   author = {Modlitbová, Pavlína and Farka, Zdeněk and Pastucha, Matěj and Pořízka, Pavel and Novotný, Karel and Skládal, Petr and Kaiser, Jozef},
   article_location = {Vienna},
   article_number = {9},
   doi = {http://dx.doi.org/10.1007/s00604-019-3742-9},
   keywords = {Collinear plasma collection; Gold nanoparticles; Laser ablation; Microtiter plate; Sandwich immunoassay; Silver nanoparticles; Streptavidin; Tag-LIBS},
   language = {eng},
   issn = {0026-3672},
   journal = {Microchimica Acta},
   title = {Laser-induced breakdown spectroscopy as a novel readout method for nanoparticle-based immunoassays},
   url = {https://link.springer.com/article/10.1007%2Fs00604-019-3742-9},
   volume = {186},
   year = {2019}
}

TY  - JOUR
ID  - 1549721
AU  - Modlitbová, Pavlína - Farka, Zdeněk - Pastucha, Matěj - Pořízka, Pavel - Novotný, Karel - Skládal, Petr - Kaiser, Jozef
PY  - 2019
TI  - Laser-induced breakdown spectroscopy as a novel readout method for nanoparticle-based immunoassays
JF  - Microchimica Acta
VL  - 186
IS  - 9
SP  - 1-10
EP  - 1-10
PB  - Springer
SN  - 00263672
KW  - Collinear plasma collection
KW  - Gold nanoparticles
KW  - Laser ablation
KW  - Microtiter plate
KW  - Sandwich immunoassay
KW  - Silver nanoparticles
KW  - Streptavidin
KW  - Tag-LIBS
UR  - https://link.springer.com/article/10.1007%2Fs00604-019-3742-9
L2  - https://link.springer.com/article/10.1007%2Fs00604-019-3742-9
N2  - Laser-induced breakdown spectroscopy (LIBS) was examined as a novel method for readout of microtiter plate immunoassays involving nanoparticles (NP). The so-called Tag-LIBS technique is a sensitive method for the detection of specific biomarkers. It was applied to the determination of NP labels using nanosecond ablation sampling. The NP labels were examined from the bottom of a standard 96-well microtiter plate. Thanks to the flexibility of LIBS instrumentation, both the plasma emission collection and the focusing optics arrangements can be collinearly arranged. The experiments showed that silver NPs and gold NPs can be readily quantified on the bottom of the microtiter plate. Utilizing this technique, a sandwich immunoassay for human serum albumin using streptavidin-coated AgNP labels was developed. The assay has a 10 ng·mL-1 detection limit which is comparable to the sensitivity of fluorometric readout. The main advantage of this LIBS technique is its wide scope in which it enables a detection of almost any type of NP labels, irrespective to any fluorescence or catalytic properties. Owing to the immediate signal response, the relatively simple instrumentation also enables assay automation. The LIBS capability of multi-elemental analyses makes it a promising and fast alternative to other readout techniques, in particular with respect to multiplexed detection of biomarkers.
ER  -

MODLITBOVÁ, Pavlína, Zdeněk FARKA, Matěj PASTUCHA, Pavel POŘÍZKA, Karel NOVOTNÝ, Petr SKLÁDAL and Jozef KAISER. Laser-induced breakdown spectroscopy as a novel readout method for nanoparticle-based immunoassays. \textit{Microchimica Acta}. Vienna: Springer, 2019, vol.~186, No~9, p.~1-10. ISSN~0026-3672. Available from: https://dx.doi.org/10.1007/s00604-019-3742-9.

Detailed Information on Publication Record