Microscopy examination of red blood and yeast cell
agglutination induced by bacterial lectins

J 2019

Microscopy examination of red blood and yeast cell agglutination induced by bacterial lectins

MRÁZKOVÁ, Jana, Lenka MALINOVSKÁ and Michaela WIMMEROVÁ

Basic information

Original name

Microscopy examination of red blood and yeast cell agglutination induced by bacterial lectins

Authors

MRÁZKOVÁ, Jana (203 Czech Republic, belonging to the institution), Lenka MALINOVSKÁ (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

PLOS ONE, Public Library of Science, 2019, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.740

RIV identification code

RIV/00216224:14740/19:00107577

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1371/journal.pone.0220318

UT WoS

000484977900081

Keywords in English

lectins; microscopy; agglutination; specificity

Abstract

V originále

Lectins are a group of ubiquitous proteins which specifically recognize and reversibly bind sugar moieties of glycoprotein and glycolipid constituents on cell surfaces. The mutagenesis approach is often employed to characterize lectin binding properties. As lectins are not enzymes, it is not easy to perform a rapid specificity screening of mutants using chromogenic substrates. Lectin-based cell agglutination is a simple and low-cost technique which can overcome most of these problems. In this work, a modified method of the agglutination of human erythrocytes and yeast cells with microscopy detection was successfully used for a specificity study of the newly prepared mutant lectin RS-IIL_A22S, which experimentally completed studies on sugar preferences of lectins in the PA-IIL family. Results showed that the sensitivity of this method is comparable with ITC, is able to determine subtle differences in lectin specificity, and works directly in cell lysates.

Links

GA18-18964S, research and development project

Name: Lektiny a jejich úloha v interakci patogen/hostitel a buněčném rozpoznávání

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/1575/2018, interní kód MU

Name: Podpora biochemického výzkumu v roce 2019

Investor: Masaryk University, Category A

90043, large research infrastructures

Name: CIISB

Citovat

MRÁZKOVÁ, Jana, Lenka MALINOVSKÁ and Michaela WIMMEROVÁ. Microscopy examination of red blood and yeast cell agglutination induced by bacterial lectins. PLOS ONE. Public Library of Science, 2019, vol. 14, No 7, p. 1-23. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0220318.

@article{1549857,
   author = {Mrázková, Jana and Malinovská, Lenka and Wimmerová, Michaela},
   article_number = {7},
   doi = {http://dx.doi.org/10.1371/journal.pone.0220318},
   keywords = {lectins; microscopy; agglutination; specificity},
   language = {eng},
   issn = {1932-6203},
   journal = {PLOS ONE},
   title = {Microscopy examination of red blood and yeast cell agglutination induced by bacterial lectins},
   url = {https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0220318&type=printable},
   volume = {14},
   year = {2019}
}

TY  - JOUR
ID  - 1549857
AU  - Mrázková, Jana - Malinovská, Lenka - Wimmerová, Michaela
PY  - 2019
TI  - Microscopy examination of red blood and yeast cell agglutination induced by bacterial lectins
JF  - PLOS ONE
VL  - 14
IS  - 7
SP  - 1-23
EP  - 1-23
PB  - Public Library of Science
SN  - 19326203
KW  - lectins
KW  - microscopy
KW  - agglutination
KW  - specificity
UR  - https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0220318&type=printable
L2  - https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0220318&type=printable
N2  - Lectins are a group of ubiquitous proteins which specifically recognize and reversibly bind sugar moieties of glycoprotein and glycolipid constituents on cell surfaces. The mutagenesis approach is often employed to characterize lectin binding properties. As lectins are not enzymes, it is not easy to perform a rapid specificity screening of mutants using chromogenic substrates. Lectin-based cell agglutination is a simple and low-cost technique which can overcome most of these problems. In this work, a modified method of the agglutination of human erythrocytes and yeast cells with microscopy detection was successfully used for a specificity study of the newly prepared mutant lectin RS-IIL_A22S, which experimentally completed studies on sugar preferences of lectins in the PA-IIL family. Results showed that the sensitivity of this method is comparable with ITC, is able to determine subtle differences in lectin specificity, and works directly in cell lysates.
ER  -

MRÁZKOVÁ, Jana, Lenka MALINOVSKÁ and Michaela WIMMEROVÁ. Microscopy examination of red blood and yeast cell agglutination induced by bacterial lectins. \textit{PLOS ONE}. Public Library of Science, 2019, vol.~14, No~7, p.~1-23. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0220318.

Detailed Information on Publication Record