Effect of heat treatment on activity of staphylococcal
enterotoxins of type A, B, and C in milk

J 2019

Effect of heat treatment on activity of staphylococcal enterotoxins of type A, B, and C in milk

NECIDOVA, L., S. BURSOVA, Danka HARUŠTIAKOVÁ, K. BOGDANOVICOVA, I. LACANIN et. al.

Basic information

Original name

Effect of heat treatment on activity of staphylococcal enterotoxins of type A, B, and C in milk

Authors

NECIDOVA, L. (203 Czech Republic), S. BURSOVA (203 Czech Republic), Danka HARUŠTIAKOVÁ (703 Slovakia, guarantor, belonging to the institution), K. BOGDANOVICOVA (203 Czech Republic) and I. LACANIN (191 Croatia)

Edition

Journal of Dairy Science, New York, Elsevier Science Inc. 2019, 0022-0302

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

40200 4.2 Animal and Dairy science

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Full Text

Impact factor

Impact factor: 3.333

RIV identification code

RIV/00216224:14310/19:00110462

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3168/jds.2018-15255

UT WoS

000464425700012

Keywords in English

heat-resistant staphylococcal enterotoxin; Staphylococcus aureus; inactivation; enzyme-linked immunofluorescent assay (ELFA)

Abstract

V originále

Intoxication by staphylococcal enterotoxins (SE) is among the most common causes of food-poisoning outbreaks resulting from the consumption of raw milk or products made thereof. The aim of our study was to analyze the thermal stability of SE and evaluate the inactivation of SE types A, B, and C (SEA, SEB, SEC) by autoclaving at 100 degrees C, 110 degrees C, and 121 degrees C. Milk samples were inoculated with 38 Staphylococcus aureus strains that possessed the ability to produce SEA, SEB, or SEC and incubated at 37 degrees C for 24 h. This incubation was followed by heat treatment at 100 degrees C, 110 degrees C, or 121 degrees C for 3 min. Samples were analyzed by Staph. aureus plate count method on Baird-Parker agar and specifically for the presence of SE. An enzyme-linked immunofluorescent assay (ELFA) on a MiniVIDAS analyzer (bioMerieux, Marcy l'Etoile, France) was used to detect SE, which were determined semi-quantitatively based on test values. The obtained results were analyzed by means of nonparametric statistical methods. All samples (100%; 38/38) were SE-positive before heat treatment, and the positivity rates decreased after heat treatment at 100 degrees C, 110 degrees C, and 121 degrees C to 36.8% (14/38), 34.2% (13/38), and 31.6% (12/38), respectively. The rates of positive samples differed between SEA, SEB, and SEC producers: SEA was detected in the highest amounts both before and after heat treatment. The amount of SE (expressed as test values) decreased significantly after heat treatment. Comparing amounts of SE in positive and negative samples before and after heat treatment, we can conclude that the success of SE inactivation depends on the amount present before heat treatment. The highest amount of SE and the highest rate of SE-positive samples after all heat treatments were found in samples with strains producing SEA. For SEB and SEC, lower amounts of enterotoxin were present and were inactivated at 100 degrees C. Although temperatures of 100 degrees C, 110 degrees C, and 121 degrees C may inactivate SE in milk, the key measures in prevention of staphylococcal enterotoxicosis are avoiding initial contamination of milk by Staph. aureus, promoting consumption of heat-treated milk, and preventing disruption of the cold chain during milk production and processing.

Citovat

NECIDOVA, L., S. BURSOVA, Danka HARUŠTIAKOVÁ, K. BOGDANOVICOVA and I. LACANIN. Effect of heat treatment on activity of staphylococcal enterotoxins of type A, B, and C in milk. Journal of Dairy Science. New York: Elsevier Science Inc., 2019, vol. 102, No 5, p. 3924-3932. ISSN 0022-0302. Available from: https://dx.doi.org/10.3168/jds.2018-15255.

@article{1549881,
   author = {Necidova, L. and Bursova, S. and Haruštiaková, Danka and Bogdanovicova, K. and Lacanin, I.},
   article_location = {New York},
   article_number = {5},
   doi = {http://dx.doi.org/10.3168/jds.2018-15255},
   keywords = {heat-resistant staphylococcal enterotoxin; Staphylococcus aureus; inactivation; enzyme-linked immunofluorescent assay (ELFA)},
   language = {eng},
   issn = {0022-0302},
   journal = {Journal of Dairy Science},
   title = {Effect of heat treatment on activity of staphylococcal enterotoxins of type A, B, and C in milk},
   url = {https://www.sciencedirect.com/science/article/pii/S0022030219302139?via%3Dihub},
   volume = {102},
   year = {2019}
}

TY  - JOUR
ID  - 1549881
AU  - Necidova, L. - Bursova, S. - Haruštiaková, Danka - Bogdanovicova, K. - Lacanin, I.
PY  - 2019
TI  - Effect of heat treatment on activity of staphylococcal enterotoxins of type A, B, and C in milk
JF  - Journal of Dairy Science
VL  - 102
IS  - 5
SP  - 3924-3932
EP  - 3924-3932
PB  - Elsevier Science Inc.
SN  - 00220302
KW  - heat-resistant staphylococcal enterotoxin
KW  - Staphylococcus aureus
KW  - inactivation
KW  - enzyme-linked immunofluorescent assay (ELFA)
UR  - https://www.sciencedirect.com/science/article/pii/S0022030219302139?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0022030219302139?via%3Dihub
N2  - Intoxication by staphylococcal enterotoxins (SE) is among the most common causes of food-poisoning outbreaks resulting from the consumption of raw milk or products made thereof. The aim of our study was to analyze the thermal stability of SE and evaluate the inactivation of SE types A, B, and C (SEA, SEB, SEC) by autoclaving at 100 degrees C, 110 degrees C, and 121 degrees C. Milk samples were inoculated with 38 Staphylococcus aureus strains that possessed the ability to produce SEA, SEB, or SEC and incubated at 37 degrees C for 24 h. This incubation was followed by heat treatment at 100 degrees C, 110 degrees C, or 121 degrees C for 3 min. Samples were analyzed by Staph. aureus plate count method on Baird-Parker agar and specifically for the presence of SE. An enzyme-linked immunofluorescent assay (ELFA) on a MiniVIDAS analyzer (bioMerieux, Marcy l'Etoile, France) was used to detect SE, which were determined semi-quantitatively based on test values. The obtained results were analyzed by means of nonparametric statistical methods. All samples (100%; 38/38) were SE-positive before heat treatment, and the positivity rates decreased after heat treatment at 100 degrees C, 110 degrees C, and 121 degrees C to 36.8% (14/38), 34.2% (13/38), and 31.6% (12/38), respectively. The rates of positive samples differed between SEA, SEB, and SEC producers: SEA was detected in the highest amounts both before and after heat treatment. The amount of SE (expressed as test values) decreased significantly after heat treatment. Comparing amounts of SE in positive and negative samples before and after heat treatment, we can conclude that the success of SE inactivation depends on the amount present before heat treatment. The highest amount of SE and the highest rate of SE-positive samples after all heat treatments were found in samples with strains producing SEA. For SEB and SEC, lower amounts of enterotoxin were present and were inactivated at 100 degrees C. Although temperatures of 100 degrees C, 110 degrees C, and 121 degrees C may inactivate SE in milk, the key measures in prevention of staphylococcal enterotoxicosis are avoiding initial contamination of milk by Staph. aureus, promoting consumption of heat-treated milk, and preventing disruption of the cold chain during milk production and processing.
ER  -

NECIDOVA, L., S. BURSOVA, Danka HARUŠTIAKOVÁ, K. BOGDANOVICOVA and I. LACANIN. Effect of heat treatment on activity of staphylococcal enterotoxins of type A, B, and C in milk. \textit{Journal of Dairy Science}. New York: Elsevier Science Inc., 2019, vol.~102, No~5, p.~3924-3932. ISSN~0022-0302. Available from: https://dx.doi.org/10.3168/jds.2018-15255.

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