Impact of acceptor splice site NAGTAG motif on exon recognition

J 2019

Impact of acceptor splice site NAGTAG motif on exon recognition

HUJOVÁ, Pavla, Lucie GRODECKÁ, Přemysl SOUČEK and Tomáš FREIBERGER

Basic information

Original name

Impact of acceptor splice site NAGTAG motif on exon recognition

Authors

HUJOVÁ, Pavla (203 Czech Republic, belonging to the institution), Lucie GRODECKÁ (203 Czech Republic), Přemysl SOUČEK (203 Czech Republic, guarantor) and Tomáš FREIBERGER (203 Czech Republic, belonging to the institution)

Edition

MOLECULAR BIOLOGY REPORTS, DORDRECHT, Springer, 2019, 0301-4851

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.402

RIV identification code

RIV/00216224:14110/19:00108505

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s11033-019-04734-6

UT WoS

000470332600028

Keywords in English

Pre-mRNA splicing; Acceptor splice site; NAGNAG motif; Tandem acceptor splice site

Abstract

V originále

Pre-mRNA splicing is an essential step in gene expression, when introns are removed and exons joined by the complex of proteins called spliceosome. Correct splicing requires a precise exon/intron junction definition, which is determined by a consensual donor and acceptor splice site at the 5 and 3 end, respectively. An acceptor splice site (3ss) consists of highly conserved AG nucleotides in positions E-2 and E-1. These nucleotides can appear in tandem, located 3bp from each other. Then they are referred to as NAGNAG or tandem 3ss, which can be alternatively spliced. NAG/TAG 3ss motif abundance is extremely low and cannot be easily explained by just a nucleotide preference in this position. We tested artificial NAG/TAG motif's potential negative effect on exon recognition using a minigene assay. Introducing the NAG/TAG motif into seven different exons revealed no general negative effect on exon recognition. The only observed effect was the partial use of the newly formed distal 3ss. We can conclude that this motif's extremely low preference in a natural 3ss is not a consequence of the NAG/TAG motif's negative effect on exon recognition, but more likely the result of other RNA processing aspects, such as an alternative 3ss choice, decreased 3ss strength, or incorporating an amber stop codon.

Links

NV16-34414A, research and development project

Name: Určení genových oblastí náchylných ke vzniku mutací ovlivňujících sestřih mRNA

Citovat

HUJOVÁ, Pavla, Lucie GRODECKÁ, Přemysl SOUČEK and Tomáš FREIBERGER. Impact of acceptor splice site NAGTAG motif on exon recognition. MOLECULAR BIOLOGY REPORTS. DORDRECHT: Springer, 2019, vol. 46, No 3, p. 2877-2884. ISSN 0301-4851. Available from: https://dx.doi.org/10.1007/s11033-019-04734-6.

@article{1550501,
   author = {Hujová, Pavla and Grodecká, Lucie and Souček, Přemysl and Freiberger, Tomáš},
   article_location = {DORDRECHT},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s11033-019-04734-6},
   keywords = {Pre-mRNA splicing; Acceptor splice site; NAGNAG motif; Tandem acceptor splice site},
   language = {eng},
   issn = {0301-4851},
   journal = {MOLECULAR BIOLOGY REPORTS},
   title = {Impact of acceptor splice site NAGTAG motif on exon recognition},
   url = {http://dx.doi.org/10.1007/s11033-019-04734-6},
   volume = {46},
   year = {2019}
}

TY  - JOUR
ID  - 1550501
AU  - Hujová, Pavla - Grodecká, Lucie - Souček, Přemysl - Freiberger, Tomáš
PY  - 2019
TI  - Impact of acceptor splice site NAGTAG motif on exon recognition
JF  - MOLECULAR BIOLOGY REPORTS
VL  - 46
IS  - 3
SP  - 2877-2884
EP  - 2877-2884
PB  - Springer
SN  - 03014851
KW  - Pre-mRNA splicing
KW  - Acceptor splice site
KW  - NAGNAG motif
KW  - Tandem acceptor splice site
UR  - http://dx.doi.org/10.1007/s11033-019-04734-6
L2  - http://dx.doi.org/10.1007/s11033-019-04734-6
N2  - Pre-mRNA splicing is an essential step in gene expression, when introns are removed and exons joined by the complex of proteins called spliceosome. Correct splicing requires a precise exon/intron junction definition, which is determined by a consensual donor and acceptor splice site at the 5 and 3 end, respectively. An acceptor splice site (3ss) consists of highly conserved AG nucleotides in positions E-2 and E-1. These nucleotides can appear in tandem, located 3bp from each other. Then they are referred to as NAGNAG or tandem 3ss, which can be alternatively spliced. NAG/TAG 3ss motif abundance is extremely low and cannot be easily explained by just a nucleotide preference in this position. We tested artificial NAG/TAG motif's potential negative effect on exon recognition using a minigene assay. Introducing the NAG/TAG motif into seven different exons revealed no general negative effect on exon recognition. The only observed effect was the partial use of the newly formed distal 3ss. We can conclude that this motif's extremely low preference in a natural 3ss is not a consequence of the NAG/TAG motif's negative effect on exon recognition, but more likely the result of other RNA processing aspects, such as an alternative 3ss choice, decreased 3ss strength, or incorporating an amber stop codon.
ER  -

HUJOVÁ, Pavla, Lucie GRODECKÁ, Přemysl SOUČEK and Tomáš FREIBERGER. Impact of acceptor splice site NAGTAG motif on exon recognition. \textit{MOLECULAR BIOLOGY REPORTS}. DORDRECHT: Springer, 2019, vol.~46, No~3, p.~2877-2884. ISSN~0301-4851. Available from: https://dx.doi.org/10.1007/s11033-019-04734-6.

Detailed Information on Publication Record