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@article{1550501, author = {Hujová, Pavla and Grodecká, Lucie and Souček, Přemysl and Freiberger, Tomáš}, article_location = {DORDRECHT}, article_number = {3}, doi = {http://dx.doi.org/10.1007/s11033-019-04734-6}, keywords = {Pre-mRNA splicing; Acceptor splice site; NAGNAG motif; Tandem acceptor splice site}, language = {eng}, issn = {0301-4851}, journal = {MOLECULAR BIOLOGY REPORTS}, title = {Impact of acceptor splice site NAGTAG motif on exon recognition}, url = {http://dx.doi.org/10.1007/s11033-019-04734-6}, volume = {46}, year = {2019} }
TY - JOUR ID - 1550501 AU - Hujová, Pavla - Grodecká, Lucie - Souček, Přemysl - Freiberger, Tomáš PY - 2019 TI - Impact of acceptor splice site NAGTAG motif on exon recognition JF - MOLECULAR BIOLOGY REPORTS VL - 46 IS - 3 SP - 2877-2884 EP - 2877-2884 PB - Springer SN - 03014851 KW - Pre-mRNA splicing KW - Acceptor splice site KW - NAGNAG motif KW - Tandem acceptor splice site UR - http://dx.doi.org/10.1007/s11033-019-04734-6 L2 - http://dx.doi.org/10.1007/s11033-019-04734-6 N2 - Pre-mRNA splicing is an essential step in gene expression, when introns are removed and exons joined by the complex of proteins called spliceosome. Correct splicing requires a precise exon/intron junction definition, which is determined by a consensual donor and acceptor splice site at the 5 and 3 end, respectively. An acceptor splice site (3ss) consists of highly conserved AG nucleotides in positions E-2 and E-1. These nucleotides can appear in tandem, located 3bp from each other. Then they are referred to as NAGNAG or tandem 3ss, which can be alternatively spliced. NAG/TAG 3ss motif abundance is extremely low and cannot be easily explained by just a nucleotide preference in this position. We tested artificial NAG/TAG motif's potential negative effect on exon recognition using a minigene assay. Introducing the NAG/TAG motif into seven different exons revealed no general negative effect on exon recognition. The only observed effect was the partial use of the newly formed distal 3ss. We can conclude that this motif's extremely low preference in a natural 3ss is not a consequence of the NAG/TAG motif's negative effect on exon recognition, but more likely the result of other RNA processing aspects, such as an alternative 3ss choice, decreased 3ss strength, or incorporating an amber stop codon. ER -
HUJOVÁ, Pavla, Lucie GRODECKÁ, Přemysl SOUČEK and Tomáš FREIBERGER. Impact of acceptor splice site NAGTAG motif on exon recognition. \textit{MOLECULAR BIOLOGY REPORTS}. DORDRECHT: Springer, 2019, vol.~46, No~3, p.~2877-2884. ISSN~0301-4851. Available from: https://dx.doi.org/10.1007/s11033-019-04734-6.
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