Egg maturity assessment prior to ICSI prevents premature
fertilization of late-maturing oocytes

J 2019

Egg maturity assessment prior to ICSI prevents premature fertilization of late-maturing oocytes

HOLUBCOVÁ, Zuzana, Drahomíra KYJOVSKÁ, Martina MARTONOVÁ, Darja PÁRALOVÁ, Tereza KLENKOVÁ et. al.

Basic information

Original name

Egg maturity assessment prior to ICSI prevents premature fertilization of late-maturing oocytes

Authors

HOLUBCOVÁ, Zuzana (203 Czech Republic, guarantor, belonging to the institution), Drahomíra KYJOVSKÁ (203 Czech Republic), Martina MARTONOVÁ (203 Czech Republic), Darja PÁRALOVÁ (203 Czech Republic), Tereza KLENKOVÁ (203 Czech Republic), Pavel OTEVŘEL (203 Czech Republic), Radka ŠTĚPÁNOVÁ (203 Czech Republic, belonging to the institution), Soňa KLOUDOVÁ (203 Czech Republic) and Aleš HAMPL (203 Czech Republic, belonging to the institution)

Edition

Journal of Assisted Reproduction and Genetics, NEW YORK, SPRINGER/PLENUM PUBLISHERS, 2019, 1058-0468

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30214 Obstetrics and gynaecology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.829

RIV identification code

RIV/00216224:14110/19:00110498

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s10815-018-1393-0

UT WoS

000463773900011

Keywords in English

Timing of ICSI; Oocyte maturation; Polarized light microscopy; Meiotic spindle; Immature oocytes

Abstract

V originále

Propose The presence of metaphase II (MII) spindle together with the polar body (PB) indicates completion of oocyte maturation. This study was designed to explore if spindle imaging can be used to optimize timing of intracytoplasmic sperm injection (ICSI). Methods The study involved 916 oocytes from 234 conventionally stimulated ICSI cycles with an unexpectedly poor ovarian response. All PB-displaying oocytes were subjected to polarized light microscopy (PLM) prior to ICSI. When MII spindle was absent in the majority of oocytes, ICSI was postponed and performed after additional spindle imaging. Fertilization, embryo development, and clinical outcome were evaluated with respect to the observed spindle pattern. Results The visible spindle was absent in 32.64% of PB-displaying oocytes. The late-maturing oocytes extruding PB in vitro were less likely to exhibit a spindle signal than in vivo matured MII oocytes (38.86% vs. 89.84%). When fertilization was postponed, 59.39% of initially spindle-negative oocytes developed detectable MII spindle. Spindled eggs had significantly higher developmental potential, and the presence of the spindle has been identified as an independent measure for predicting the formation of the blastocyst. Embryos derived from spindle-positive oocytes also showed a higher chance to implant and develop to term. Notably, 11 children were conceived by finely timed fertilization of late-maturing oocytes which are normally discarded. Conclusions The study confirms the prognostic value of spindle imaging and demonstrates that immature oocytes can be clinically utilized and give rise to live births when the timing of ICSI is adjusted to their developmental stage.

Citovat

HOLUBCOVÁ, Zuzana, Drahomíra KYJOVSKÁ, Martina MARTONOVÁ, Darja PÁRALOVÁ, Tereza KLENKOVÁ, Pavel OTEVŘEL, Radka ŠTĚPÁNOVÁ, Soňa KLOUDOVÁ and Aleš HAMPL. Egg maturity assessment prior to ICSI prevents premature fertilization of late-maturing oocytes. Journal of Assisted Reproduction and Genetics. NEW YORK: SPRINGER/PLENUM PUBLISHERS, 2019, vol. 36, No 3, p. 445-452. ISSN 1058-0468. Available from: https://dx.doi.org/10.1007/s10815-018-1393-0.

@article{1550537,
   author = {Holubcová, Zuzana and Kyjovská, Drahomíra and Martonová, Martina and Páralová, Darja and Klenková, Tereza and Otevřel, Pavel and Štěpánová, Radka and Kloudová, Soňa and Hampl, Aleš},
   article_location = {NEW YORK},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s10815-018-1393-0},
   keywords = {Timing of ICSI; Oocyte maturation; Polarized light microscopy; Meiotic spindle; Immature oocytes},
   language = {eng},
   issn = {1058-0468},
   journal = {Journal of Assisted Reproduction and Genetics},
   title = {Egg maturity assessment prior to ICSI prevents premature fertilization of late-maturing oocytes},
   url = {https://link.springer.com/article/10.1007%2Fs10815-018-1393-0},
   volume = {36},
   year = {2019}
}

TY  - JOUR
ID  - 1550537
AU  - Holubcová, Zuzana - Kyjovská, Drahomíra - Martonová, Martina - Páralová, Darja - Klenková, Tereza - Otevřel, Pavel - Štěpánová, Radka - Kloudová, Soňa - Hampl, Aleš
PY  - 2019
TI  - Egg maturity assessment prior to ICSI prevents premature fertilization of late-maturing oocytes
JF  - Journal of Assisted Reproduction and Genetics
VL  - 36
IS  - 3
SP  - 445-452
EP  - 445-452
PB  - SPRINGER/PLENUM PUBLISHERS
SN  - 10580468
KW  - Timing of ICSI
KW  - Oocyte maturation
KW  - Polarized light microscopy
KW  - Meiotic spindle
KW  - Immature oocytes
UR  - https://link.springer.com/article/10.1007%2Fs10815-018-1393-0
L2  - https://link.springer.com/article/10.1007%2Fs10815-018-1393-0
N2  - Propose The presence of metaphase II (MII) spindle together with the polar body (PB) indicates completion of oocyte maturation. This study was designed to explore if spindle imaging can be used to optimize timing of intracytoplasmic sperm injection (ICSI). Methods The study involved 916 oocytes from 234 conventionally stimulated ICSI cycles with an unexpectedly poor ovarian response. All PB-displaying oocytes were subjected to polarized light microscopy (PLM) prior to ICSI. When MII spindle was absent in the majority of oocytes, ICSI was postponed and performed after additional spindle imaging. Fertilization, embryo development, and clinical outcome were evaluated with respect to the observed spindle pattern. Results The visible spindle was absent in 32.64% of PB-displaying oocytes. The late-maturing oocytes extruding PB in vitro were less likely to exhibit a spindle signal than in vivo matured MII oocytes (38.86% vs. 89.84%). When fertilization was postponed, 59.39% of initially spindle-negative oocytes developed detectable MII spindle. Spindled eggs had significantly higher developmental potential, and the presence of the spindle has been identified as an independent measure for predicting the formation of the blastocyst. Embryos derived from spindle-positive oocytes also showed a higher chance to implant and develop to term. Notably, 11 children were conceived by finely timed fertilization of late-maturing oocytes which are normally discarded. Conclusions The study confirms the prognostic value of spindle imaging and demonstrates that immature oocytes can be clinically utilized and give rise to live births when the timing of ICSI is adjusted to their developmental stage.
ER  -

HOLUBCOVÁ, Zuzana, Drahomíra KYJOVSKÁ, Martina MARTONOVÁ, Darja PÁRALOVÁ, Tereza KLENKOVÁ, Pavel OTEVŘEL, Radka ŠTĚPÁNOVÁ, Soňa KLOUDOVÁ and Aleš HAMPL. Egg maturity assessment prior to ICSI prevents premature fertilization of late-maturing oocytes. \textit{Journal of Assisted Reproduction and Genetics}. NEW YORK: SPRINGER/PLENUM PUBLISHERS, 2019, vol.~36, No~3, p.~445-452. ISSN~1058-0468. Available from: https://dx.doi.org/10.1007/s10815-018-1393-0.

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