a 2019

Age determination in individual wild-caught Cimex lectularius using pteridine concentration

KŘEMENOVÁ, Jana, Tomáš BARTONIČKA and Zdeněk ŠIMEK

Basic information

Original name

Age determination in individual wild-caught Cimex lectularius using pteridine concentration

Authors

KŘEMENOVÁ, Jana (203 Czech Republic, guarantor, belonging to the institution), Tomáš BARTONIČKA (203 Czech Republic, belonging to the institution) and Zdeněk ŠIMEK (203 Czech Republic, belonging to the institution)

Edition

3rd Evolutionary Ecology PhD meeting, 2019

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10613 Zoology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/19:00107601

Organization unit

Faculty of Science

Keywords in English

pteridines; age determining; insect

Tags

International impact
Změněno: 12/9/2019 13:34, Mgr. Jana Vandrovcová, Ph.D.

Abstract

V originále

The individual age for different insect species can be determined by the concentration of pteridines in the eye. The female age and with it the changing ability to keep viable sperm seems to be an important parameter in the study of ecological speciation and reproductive isolation of two host lines of bedbugs (C. lectularius) parasitizing humans and bats. No studies have yet been conducted to determine the age of the bed bug and there are still some methodological inconsistencies in determining the age we had to deal with, i.e. the selection of pteridine standards and extraction method, the presence of light during extraction, before we could proceed to compile a calibration curve for the dependence of pteridines on age. After optimizing the extraction and measurement of pteridine concentrations, we developed new methodological protocol. Of the five tested pteridine standards, Leucopterin showed the highest consistency within the age cohort and seems be most appropriate for determining the age of bedbugs in wild populations.

Links

GC18-08468J, research and development project
Name: Role adaptace a fenotypové plasticity spermií v ekologické speciaci
Investor: Czech Science Foundation