Roles of RAD51 and RTEL1 in telomere and rDNA stability in
Physcomitrella patens

J 2019

Roles of RAD51 and RTEL1 in telomere and rDNA stability in Physcomitrella patens

GOFFOVÁ, Ivana, Radka VÁGNEROVÁ, Vratislav PEŠKA, Michal FRANEK, Kateřina HAVLOVÁ et. al.

Basic information

Original name

Roles of RAD51 and RTEL1 in telomere and rDNA stability in Physcomitrella patens

Authors

GOFFOVÁ, Ivana (703 Slovakia, belonging to the institution), Radka VÁGNEROVÁ (203 Czech Republic), Vratislav PEŠKA (203 Czech Republic), Michal FRANEK (703 Slovakia, belonging to the institution), Kateřina HAVLOVÁ (203 Czech Republic, belonging to the institution), Marcela HOLÁ (203 Czech Republic), Dagmar ZACHOVÁ (203 Czech Republic, belonging to the institution), Miloslava FOJTOVÁ (203 Czech Republic, belonging to the institution), Andrew CUMING (826 United Kingdom of Great Britain and Northern Ireland), Yasuko KAMISUGI (826 United Kingdom of Great Britain and Northern Ireland), Karel J. ANGELIS (203 Czech Republic) and Jiří FAJKUS (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plant Journal, Oxford, Blackwell Publishing Ltd, 2019, 0960-7412

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.14304

Impact factor

Impact factor: 6.141

RIV identification code

RIV/00216224:14740/19:00107607

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1111/tpj.14304

UT WoS

000473644100011

Keywords (in Czech)

Physcomitrella patens; ribosomální RNA geny; telomery; stabilita genomu; RTEL1; RAD51; Sog One-Like

Keywords in English

Physcomitrella patens; ribosomal RNA genes; telomere; genome stability; RTEL1; RAD51; Sog One-Like

Abstract

V originále

Telomeres and ribosomal RNA genes (rDNA) are essential for cell survival and particularly sensitive to factors affecting genome stability. Here, we examine the role of RAD51 and its antagonist, RTEL1, in the moss Physcomitrella patens. In corresponding mutants, we analyse their sensitivity to DNA damage, the maintenance of telomeres and rDNA, and repair of double-stranded breaks (DSBs) induced by genotoxins with various modes of action. While the loss of RTEL1 results in rapid telomere shortening, concurrent loss of both RAD51 genes has no effect on telomere lengths. We further demonstrate here the linked arrangement of 5S and 45S rRNA genes in P. patens. The spacer between 5S and 18S rRNA genes, especially the region downstream from the transcription start site, shows conspicuous clustering of sites with a high propensity to form quadruplex (G4) structures. Copy numbers of 5S and 18S rDNA are reduced moderately in the pprtel1 mutant, and significantly in the double pprad51-1-2 mutant, with no progression during subsequent cultivation. While reductions in 45S rDNA copy numbers observed in pprtel1 and pprad51-1-2 plants apply also to 5S rDNA, changes in transcript levels are different for 45S and 5S rRNA, indicating their independent transcription by RNA polymerase I and III, respectively. The loss of SOL (Sog One-Like), a transcription factor regulating numerous genes involved in DSB repair, increases the rate of DSB repair in dividing as well as differentiated tissue, and through deactivation of G2/M cell-cycle checkpoint allows the cell-cycle progression manifested as a phenotype resistant to bleomycin.

Links

GA16-01137S, research and development project

Name: Faktory genomové stability u mechu a vyšších rostlin

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

90062, large research infrastructures

Name: Czech-BioImaging

Citovat

GOFFOVÁ, Ivana, Radka VÁGNEROVÁ, Vratislav PEŠKA, Michal FRANEK, Kateřina HAVLOVÁ, Marcela HOLÁ, Dagmar ZACHOVÁ, Miloslava FOJTOVÁ, Andrew CUMING, Yasuko KAMISUGI, Karel J. ANGELIS and Jiří FAJKUS. Roles of RAD51 and RTEL1 in telomere and rDNA stability in Physcomitrella patens. Plant Journal. Oxford: Blackwell Publishing Ltd, 2019, vol. 98, No 6, p. 1090-1105. ISSN 0960-7412. Available from: https://dx.doi.org/10.1111/tpj.14304.

@article{1551679,
   author = {Goffová, Ivana and Vágnerová, Radka and Peška, Vratislav and Franek, Michal and Havlová, Kateřina and Holá, Marcela and Zachová, Dagmar and Fojtová, Miloslava and Cuming, Andrew and Kamisugi, Yasuko and Angelis, Karel J. and Fajkus, Jiří},
   article_location = {Oxford},
   article_number = {6},
   doi = {http://dx.doi.org/10.1111/tpj.14304},
   keywords = {Physcomitrella patens; ribosomal RNA genes; telomere; genome stability; RTEL1; RAD51; Sog One-Like},
   language = {eng},
   issn = {0960-7412},
   journal = {Plant Journal},
   title = {Roles of RAD51 and RTEL1 in telomere and rDNA stability in Physcomitrella patens},
   url = {https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.14304},
   volume = {98},
   year = {2019}
}

TY  - JOUR
ID  - 1551679
AU  - Goffová, Ivana - Vágnerová, Radka - Peška, Vratislav - Franek, Michal - Havlová, Kateřina - Holá, Marcela - Zachová, Dagmar - Fojtová, Miloslava - Cuming, Andrew - Kamisugi, Yasuko - Angelis, Karel J. - Fajkus, Jiří
PY  - 2019
TI  - Roles of RAD51 and RTEL1 in telomere and rDNA stability in Physcomitrella patens
JF  - Plant Journal
VL  - 98
IS  - 6
SP  - 1090-1105
EP  - 1090-1105
PB  - Blackwell Publishing Ltd
SN  - 09607412
KW  - Physcomitrella patens
KW  - ribosomal RNA genes
KW  - telomere
KW  - genome stability
KW  - RTEL1
KW  - RAD51
KW  - Sog One-Like
UR  - https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.14304
L2  - https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.14304
N2  - Telomeres and ribosomal RNA genes (rDNA) are essential for cell survival and particularly sensitive to factors affecting genome stability. Here, we examine the role of RAD51 and its antagonist, RTEL1, in the moss Physcomitrella patens. In corresponding mutants, we analyse their sensitivity to DNA damage, the maintenance of telomeres and rDNA, and repair of double-stranded breaks (DSBs) induced by genotoxins with various modes of action. While the loss of RTEL1 results in rapid telomere shortening, concurrent loss of both RAD51 genes has no effect on telomere lengths. We further demonstrate here the linked arrangement of 5S and 45S rRNA genes in P. patens. The spacer between 5S and 18S rRNA genes, especially the region downstream from the transcription start site, shows conspicuous clustering of sites with a high propensity to form quadruplex (G4) structures. Copy numbers of 5S and 18S rDNA are reduced moderately in the pprtel1 mutant, and significantly in the double pprad51-1-2 mutant, with no progression during subsequent cultivation. While reductions in 45S rDNA copy numbers observed in pprtel1 and pprad51-1-2 plants apply also to 5S rDNA, changes in transcript levels are different for 45S and 5S rRNA, indicating their independent transcription by RNA polymerase I and III, respectively. The loss of SOL (Sog One-Like), a transcription factor regulating numerous genes involved in DSB repair, increases the rate of DSB repair in dividing as well as differentiated tissue, and through deactivation of G2/M cell-cycle checkpoint allows the cell-cycle progression manifested as a phenotype resistant to bleomycin.
ER  -

GOFFOVÁ, Ivana, Radka VÁGNEROVÁ, Vratislav PEŠKA, Michal FRANEK, Kateřina HAVLOVÁ, Marcela HOLÁ, Dagmar ZACHOVÁ, Miloslava FOJTOVÁ, Andrew CUMING, Yasuko KAMISUGI, Karel J. ANGELIS and Jiří FAJKUS. Roles of RAD51 and RTEL1 in telomere and rDNA stability in Physcomitrella patens. \textit{Plant Journal}. Oxford: Blackwell Publishing Ltd, 2019, vol.~98, No~6, p.~1090-1105. ISSN~0960-7412. Available from: https://dx.doi.org/10.1111/tpj.14304.

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