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@article{1551679,
author = {Goffová, Ivana and Vágnerová, Radka and Peška, Vratislav and Franek, Michal and Havlová, Kateřina and Holá, Marcela and Zachová, Dagmar and Fojtová, Miloslava and Cuming, Andrew and Kamisugi, Yasuko and Angelis, Karel J. and Fajkus, Jiří},
article_location = {Oxford},
article_number = {6},
doi = {http://dx.doi.org/10.1111/tpj.14304},
keywords = {Physcomitrella patens; ribosomal RNA genes; telomere; genome stability; RTEL1; RAD51; Sog One-Like},
language = {eng},
issn = {0960-7412},
journal = {Plant Journal},
title = {Roles of RAD51 and RTEL1 in telomere and rDNA stability in Physcomitrella patens},
url = {https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.14304},
volume = {98},
year = {2019}
}
TY - JOUR
ID - 1551679
AU - Goffová, Ivana - Vágnerová, Radka - Peška, Vratislav - Franek, Michal - Havlová, Kateřina - Holá, Marcela - Zachová, Dagmar - Fojtová, Miloslava - Cuming, Andrew - Kamisugi, Yasuko - Angelis, Karel J. - Fajkus, Jiří
PY - 2019
TI - Roles of RAD51 and RTEL1 in telomere and rDNA stability in Physcomitrella patens
JF - Plant Journal
VL - 98
IS - 6
SP - 1090-1105
EP - 1090-1105
PB - Blackwell Publishing Ltd
SN - 09607412
KW - Physcomitrella patens
KW - ribosomal RNA genes
KW - telomere
KW - genome stability
KW - RTEL1
KW - RAD51
KW - Sog One-Like
UR - https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.14304
L2 - https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.14304
N2 - Telomeres and ribosomal RNA genes (rDNA) are essential for cell survival and particularly sensitive to factors affecting genome stability. Here, we examine the role of RAD51 and its antagonist, RTEL1, in the moss Physcomitrella patens. In corresponding mutants, we analyse their sensitivity to DNA damage, the maintenance of telomeres and rDNA, and repair of double-stranded breaks (DSBs) induced by genotoxins with various modes of action. While the loss of RTEL1 results in rapid telomere shortening, concurrent loss of both RAD51 genes has no effect on telomere lengths. We further demonstrate here the linked arrangement of 5S and 45S rRNA genes in P. patens. The spacer between 5S and 18S rRNA genes, especially the region downstream from the transcription start site, shows conspicuous clustering of sites with a high propensity to form quadruplex (G4) structures. Copy numbers of 5S and 18S rDNA are reduced moderately in the pprtel1 mutant, and significantly in the double pprad51-1-2 mutant, with no progression during subsequent cultivation. While reductions in 45S rDNA copy numbers observed in pprtel1 and pprad51-1-2 plants apply also to 5S rDNA, changes in transcript levels are different for 45S and 5S rRNA, indicating their independent transcription by RNA polymerase I and III, respectively. The loss of SOL (Sog One-Like), a transcription factor regulating numerous genes involved in DSB repair, increases the rate of DSB repair in dividing as well as differentiated tissue, and through deactivation of G2/M cell-cycle checkpoint allows the cell-cycle progression manifested as a phenotype resistant to bleomycin.
ER -
GOFFOVÁ, Ivana, Radka VÁGNEROVÁ, Vratislav PEŠKA, Michal FRANEK, Kateřina HAVLOVÁ, Marcela HOLÁ, Dagmar ZACHOVÁ, Miloslava FOJTOVÁ, Andrew CUMING, Yasuko KAMISUGI, Karel J. ANGELIS and Jiří FAJKUS. Roles of RAD51 and RTEL1 in telomere and rDNA stability in Physcomitrella patens. \textit{Plant Journal}. Oxford: Blackwell Publishing Ltd, 2019, vol.~98, No~6, p.~1090-1105. ISSN~0960-7412. Available from: https://dx.doi.org/10.1111/tpj.14304.
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