Identification of ability to form biofilm in Candida parapsilosis and Staphylococcus epidermidis by Raman spectroscopy

REBROŠOVÁ, Katarína, Martin ŠILER, Ota SAMEK, Filip RŮŽIČKA, Silvie BERNATOVÁ, Jan JEŽEK, Pavel ZEMÁNEK and Veronika HOLÁ. Identification of ability to form biofilm in Candida parapsilosis and Staphylococcus epidermidis by Raman spectroscopy. FUTURE MICROBIOLOGY. LONDON: FUTURE MEDICINE LTD, 2019, vol. 14, No 6, p. 509-518. ISSN 1746-0913. Available from: https://dx.doi.org/10.2217/fmb-2018-0297.

Basic information

• Original name: Identification of ability to form biofilm in Candida parapsilosis and Staphylococcus epidermidis by Raman spectroscopy
• Authors: REBROŠOVÁ, Katarína (703 Slovakia, belonging to the institution), Martin ŠILER (203 Czech Republic), Ota SAMEK (203 Czech Republic), Filip RŮŽIČKA (203 Czech Republic, belonging to the institution), Silvie BERNATOVÁ (203 Czech Republic), Jan JEŽEK (203 Czech Republic), Pavel ZEMÁNEK (203 Czech Republic) and Veronika HOLÁ (203 Czech Republic, belonging to the institution).
• Edition: FUTURE MICROBIOLOGY, LONDON, FUTURE MEDICINE LTD, 2019, 1746-0913.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10606 Microbiology
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 2.907
• RIV identification code: RIV/00216224:14110/19:00115102
• Organization unit: Faculty of Medicine
• Doi: http://dx.doi.org/10.2217/fmb-2018-0297
• UT WoS: 000476570800007
• Keywords in English: biofilm; Candida parapsilosis; Raman spectroscopy; rapid diagnostics; Staphylococcus epidermidis
• Tags: 14110113, rivok
• Tags: International impact, Reviewed

Abstract

Aim: Finding rapid, reliable diagnostic methods is a big challenge in clinical microbiology. Raman spectroscopy is an optical method used for multiple applications in scientific fields including microbiology. This work reports its potential in identifying biofilm positive strains of Candida parapsilosis and Staphylococcus epidermidis. Materials & methods: We tested 54 S. epidermidis strains (23 biofilm positive, 31 negative) and 51 C. parapsilosis strains (27 biofilm positive, 24 negative) from colonies on Mueller-Hinton agar plates, using Raman spectroscopy. Results: The accuracy was 98.9% for C. parapsilosis and 96.1% for S. epidermidis. Conclusion: Themethod showed great potential for identifying biofilm positive bacterial and yeast strains. We suggest that Raman spectroscopy might become a useful aid in clinical diagnostics.

Links

• MUNI/A/0955/2016, interní kód MU: Name: Optimalizace diagnostiky původců nozokomiálních nákaz testování nových možností antimikrobiální terapie

Investor: Masaryk University, Category A

• MUNI/A/1189/2018, interní kód MU: Name: Původci nozokomiálních nákaz a možnosti jejich diagnostiky a terapie (Acronym: PNNMDT)

Investor: Masaryk University, Category A

• NV16-29916A, research and development project: Name: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu
• NV16-31593A, research and development project: Name: Polymikrobiální biofilm jako komplexní mikrobiom v rámci léčby nozokomiálních infekcí