Identification of ability to form biofilm in Candida
parapsilosis and Staphylococcus epidermidis by Raman
spectroscopy

J 2019

Identification of ability to form biofilm in Candida parapsilosis and Staphylococcus epidermidis by Raman spectroscopy

REBROŠOVÁ, Katarína, Martin ŠILER, Ota SAMEK, Filip RŮŽIČKA, Silvie BERNATOVÁ et. al.

Basic information

Original name

Identification of ability to form biofilm in Candida parapsilosis and Staphylococcus epidermidis by Raman spectroscopy

Authors

REBROŠOVÁ, Katarína (703 Slovakia, belonging to the institution), Martin ŠILER (203 Czech Republic), Ota SAMEK (203 Czech Republic), Filip RŮŽIČKA (203 Czech Republic, belonging to the institution), Silvie BERNATOVÁ (203 Czech Republic), Jan JEŽEK (203 Czech Republic), Pavel ZEMÁNEK (203 Czech Republic) and Veronika HOLÁ (203 Czech Republic, belonging to the institution)

Edition

FUTURE MICROBIOLOGY, LONDON, FUTURE MEDICINE LTD, 2019, 1746-0913

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.907

RIV identification code

RIV/00216224:14110/19:00115102

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.2217/fmb-2018-0297

UT WoS

000476570800007

Keywords in English

biofilm; Candida parapsilosis; Raman spectroscopy; rapid diagnostics; Staphylococcus epidermidis

Abstract

V originále

Aim: Finding rapid, reliable diagnostic methods is a big challenge in clinical microbiology. Raman spectroscopy is an optical method used for multiple applications in scientific fields including microbiology. This work reports its potential in identifying biofilm positive strains of Candida parapsilosis and Staphylococcus epidermidis. Materials & methods: We tested 54 S. epidermidis strains (23 biofilm positive, 31 negative) and 51 C. parapsilosis strains (27 biofilm positive, 24 negative) from colonies on Mueller-Hinton agar plates, using Raman spectroscopy. Results: The accuracy was 98.9% for C. parapsilosis and 96.1% for S. epidermidis. Conclusion: Themethod showed great potential for identifying biofilm positive bacterial and yeast strains. We suggest that Raman spectroscopy might become a useful aid in clinical diagnostics.

Links

MUNI/A/0955/2016, interní kód MU

Name: Optimalizace diagnostiky původců nozokomiálních nákaz testování nových možností antimikrobiální terapie

Investor: Masaryk University, Category A

MUNI/A/1189/2018, interní kód MU

Name: Původci nozokomiálních nákaz a možnosti jejich diagnostiky a terapie (Acronym: PNNMDT)

Investor: Masaryk University, Category A

NV16-29916A, research and development project

Name: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

NV16-31593A, research and development project

Name: Polymikrobiální biofilm jako komplexní mikrobiom v rámci léčby nozokomiálních infekcí

Citovat

REBROŠOVÁ, Katarína, Martin ŠILER, Ota SAMEK, Filip RŮŽIČKA, Silvie BERNATOVÁ, Jan JEŽEK, Pavel ZEMÁNEK and Veronika HOLÁ. Identification of ability to form biofilm in Candida parapsilosis and Staphylococcus epidermidis by Raman spectroscopy. FUTURE MICROBIOLOGY. LONDON: FUTURE MEDICINE LTD, 2019, vol. 14, No 6, p. 509-518. ISSN 1746-0913. Available from: https://dx.doi.org/10.2217/fmb-2018-0297.

@article{1552336,
   author = {Rebrošová, Katarína and Šiler, Martin and Samek, Ota and Růžička, Filip and Bernatová, Silvie and Ježek, Jan and Zemánek, Pavel and Holá, Veronika},
   article_location = {LONDON},
   article_number = {6},
   doi = {http://dx.doi.org/10.2217/fmb-2018-0297},
   keywords = {biofilm; Candida parapsilosis; Raman spectroscopy; rapid diagnostics; Staphylococcus epidermidis},
   language = {eng},
   issn = {1746-0913},
   journal = {FUTURE MICROBIOLOGY},
   title = {Identification of ability to form biofilm in Candida parapsilosis and Staphylococcus epidermidis by Raman spectroscopy},
   url = {http://dx.doi.org/10.2217/fmb-2018-0297},
   volume = {14},
   year = {2019}
}

TY  - JOUR
ID  - 1552336
AU  - Rebrošová, Katarína - Šiler, Martin - Samek, Ota - Růžička, Filip - Bernatová, Silvie - Ježek, Jan - Zemánek, Pavel - Holá, Veronika
PY  - 2019
TI  - Identification of ability to form biofilm in Candida parapsilosis and Staphylococcus epidermidis by Raman spectroscopy
JF  - FUTURE MICROBIOLOGY
VL  - 14
IS  - 6
SP  - 509-518
EP  - 509-518
PB  - FUTURE MEDICINE LTD
SN  - 17460913
KW  - biofilm
KW  - Candida parapsilosis
KW  - Raman spectroscopy
KW  - rapid diagnostics
KW  - Staphylococcus epidermidis
UR  - http://dx.doi.org/10.2217/fmb-2018-0297
L2  - http://dx.doi.org/10.2217/fmb-2018-0297
N2  - Aim: Finding rapid, reliable diagnostic methods is a big challenge in clinical microbiology. Raman spectroscopy is an optical method used for multiple applications in scientific fields including microbiology. This work reports its potential in identifying biofilm positive strains of Candida parapsilosis and Staphylococcus epidermidis. Materials & methods: We tested 54 S. epidermidis strains (23 biofilm positive, 31 negative) and 51 C. parapsilosis strains (27 biofilm positive, 24 negative) from colonies on Mueller-Hinton agar plates, using Raman spectroscopy. Results: The accuracy was 98.9% for C. parapsilosis and 96.1% for S. epidermidis. Conclusion: Themethod showed great potential for identifying biofilm positive bacterial and yeast strains. We suggest that Raman spectroscopy might become a useful aid in clinical diagnostics.
ER  -

REBROŠOVÁ, Katarína, Martin ŠILER, Ota SAMEK, Filip RŮŽIČKA, Silvie BERNATOVÁ, Jan JEŽEK, Pavel ZEMÁNEK and Veronika HOLÁ. Identification of ability to form biofilm in Candida parapsilosis and Staphylococcus epidermidis by Raman spectroscopy. \textit{FUTURE MICROBIOLOGY}. LONDON: FUTURE MEDICINE LTD, 2019, vol.~14, No~6, p.~509-518. ISSN~1746-0913. Available from: https://dx.doi.org/10.2217/fmb-2018-0297.

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