Detailed Information on Publication Record
2019
Laser-Induced Breakdown Spectroscopy as a Novel Readout Method for Nanoparticle-Based Immunoassays
MODLITBOVÁ, Pavlína, Zdeněk FARKA, Matěj PASTUCHA, Pavel POŘÍZKA, Karel NOVOTNÝ et. al.Basic information
Original name
Laser-Induced Breakdown Spectroscopy as a Novel Readout Method for Nanoparticle-Based Immunoassays
Authors
MODLITBOVÁ, Pavlína, Zdeněk FARKA, Matěj PASTUCHA, Pavel POŘÍZKA, Karel NOVOTNÝ, Petr SKLÁDAL and Jozef KAISER
Edition
EMSLIBS 2019: 10th Euro-Mediterranean Symposium on Laser-Induced Breakdown Spectroscopy, 2019
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10406 Analytical chemistry
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Organization unit
Central European Institute of Technology
ISBN
978-80-88195-13-9
Tags
International impact
Změněno: 7/9/2019 12:53, doc. Mgr. Zdeněk Farka, Ph.D.
Abstract
V originále
Laser-Induced Breakdown Spectroscopy (LIBS) was examined as a novel method to provide the readout of microtiter plate immunoassays involving nanoparticles (NP). The so-called Tag-LIBS, i.e. a sensitive detection of specific biomarkers, was applied to detect the NP labels using the nanosecond ablation sampling. The NP labels were examined from the bottom of the standard 96-well microtiter plate. Thanks to the well-known flexibility in the LIBS instrumentation, the plasma emission collection as well as the focusing optics can be rebuilt into a collinear arrangement. The presented experiments proved the suitable detection of silver NPs from the microtiter plate’s bottom without any negative effects on the NPs’ qualitative and quantitative analysis. Subsequently, a sandwich immunoassay tha tdetects human serum albumin was successfully developed, using streptavidin-coated AgNP labels. The assay provided a detection limit of 10 ng/mL, which is comparable with the fluorescence-based readout. The main advantage of LIBS readout is that it enables a detection of any type of NP labels independent on their fluorescence or catalytic properties. Owing to the immediate signal response, a relatively simple instrumentation enabling the automation, and the capability of multi-elemental analyses, LIBS technique offers a promising and fast alternative to other readout techniques allowing a multiplex detection of various biomarkers.
Links
LM2015043, research and development project |
| ||
LQ1601, research and development project |
|