LEVIN, Shahaf, Petr DUBOVÝ and Marek JOUKAL. Expression of sodium channel NaV 1.8 in dorsal root ganglia after Paclitaxel treatment. In Morphology 2019. 2019. ISBN 978-80-8152-758-6.
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Basic information
Original name Expression of sodium channel NaV 1.8 in dorsal root ganglia after Paclitaxel treatment
Authors LEVIN, Shahaf (376 Israel, belonging to the institution), Petr DUBOVÝ (203 Czech Republic, belonging to the institution) and Marek JOUKAL (203 Czech Republic, guarantor, belonging to the institution).
Edition Morphology 2019, 2019.
Other information
Original language English
Type of outcome Presentations at conferences
Field of Study 30106 Anatomy and morphology
Country of publisher Slovakia
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14110/19:00110621
Organization unit Faculty of Medicine
ISBN 978-80-8152-758-6
Keywords in English DRG; Dorsal Root Ganglia; Paclitaxel; Taxol; NaV; NaV1.8; 1.8; Peripheral Neuropathy
Tags International impact
Changed by Changed by: MUDr. Shahaf Levin, učo 443729. Changed: 27/11/2020 08:29.
Abstract
Introduction: The chemotherapeutic agent Paclitaxel causes the adverse reaction of peripheral neuropathy. The Nav sodium channel family has an important role in pain sensation. The subtype Nav 1.8 is the more abundantly expressed Tetrodotoxin resistant (TTX-R) sodium channel expressed in nociceptive Dorsal Root Ganglia (DRG) neurons. The aim of our study was to assess possible changes in Nav 1.8 channel expression in the DRG after intraperitoneal Paclitaxel application. Material and methods: Wistar rats (n=27, males) were used in our experiments. Intraperitoneal injections of Paclitaxel in 4 doses with a cumulative dose of 8 mg/kg were performed on experimental rats, while control animals received only the excipient (alcohol and cremophor EL; 1:1). The animals were left to survive for 1, 7, 14 and 21 days from the last application. Experimental and control rats were sacrificed together with naive rats and perfused transcardially by Zambonis fixative. Longitudinal cryostat sections through the lumbar DRGs were cut and immunostained for Nav 1.8. The intensity of Nav 1.8 immunofluorescence in the DRGs was measured and statistically analyzed. Results: The presence of Nav 1.8 immunofluorescence was found in lumbar DRG neurons in naive, control and Paclitaxel treated animals. Statistically significant increase in Nav 1.8 immunofluorescence was found in the DRGs of Paclitaxel treated animals in comparison to both control and naive animals. Interestingly, positivity was also detected in vessels walls within the DRG. Conclusions: Our results indicate that DRG neurons react to Paclitaxel application by increasing Nav 1.8 channel expression. These changes might be associated with the symptoms of chemotherapy induced peripheral neuropathy.
Links
MUNI/A/1086/2018, interní kód MUName: Reakce struktur nervové soustavy na poškození
Investor: Masaryk University, Category A
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