Understanding the methyl-TROSY effect over a wide range of
magnetic fields

J 2019

Understanding the methyl-TROSY effect over a wide range of magnetic fields

BOLIK-COULON, N., A.F. COUSIN, Pavel KADEŘÁVEK, J.N. DUMEZ, F. FERRAGE et. al.

Basic information

Original name

Understanding the methyl-TROSY effect over a wide range of magnetic fields

Authors

BOLIK-COULON, N. (250 France), A.F. COUSIN (250 France), Pavel KADEŘÁVEK (203 Czech Republic, guarantor, belonging to the institution), J.N. DUMEZ (250 France) and F. FERRAGE (250 France)

Edition

Journal of Chemical Physics, MELVILLE, American Institute of Physics, 2019, 0021-9606

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10403 Physical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.991

RIV identification code

RIV/00216224:14740/19:00110669

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1063/1.5095757

UT WoS

000471692400018

Keywords in English

MOLECULAR-WEIGHT PROTEINS; SIDE-CHAIN DYNAMICS; MODEL-FREE APPROACH; NMR-SPECTROSCOPY; RELAXATION; C-13; MACROMOLECULES; STRATEGIES; MOTIONS

Abstract

V originále

The use of relaxation interference in the methyl Transverse Relaxation-Optimized SpectroscopY (TROSY) experiment has opened new avenues for the study of large proteins and protein assemblies in nuclear magnetic resonance. So far, the theoretical description of the methyl-TROSY experiment has been limited to the slow-tumbling approximation, which is correct for large proteins on high-field spectrometers. In a recent paper, favorable relaxation interference was observed in the methyl groups of a small protein at a magnetic field as low as 0.33 T, well outside the slow-tumbling regime. Here, we present a model to describe relaxation interference in methyl groups over a broad range of magnetic fields, not limited to the slow-tumbling regime. We predict that the type of multiple-quantum transition that shows favorable relaxation properties change with the magnetic field. Under the condition of fast methyl-group rotation, methyl-TROSY experiments can be recorded over the entire range of magnetic fields from a fraction of 1 T up to 100 T.

Links

90043, large research infrastructures

Name: CIISB

Citovat

BOLIK-COULON, N., A.F. COUSIN, Pavel KADEŘÁVEK, J.N. DUMEZ and F. FERRAGE. Understanding the methyl-TROSY effect over a wide range of magnetic fields. Journal of Chemical Physics. MELVILLE: American Institute of Physics, 2019, vol. 150, No 22, p. 224202-224213. ISSN 0021-9606. Available from: https://dx.doi.org/10.1063/1.5095757.

@article{1556101,
   author = {BolikandCoulon, N. and Cousin, A.F. and Kadeřávek, Pavel and Dumez, J.N. and Ferrage, F.},
   article_location = {MELVILLE},
   article_number = {22},
   doi = {http://dx.doi.org/10.1063/1.5095757},
   keywords = {MOLECULAR-WEIGHT PROTEINS; SIDE-CHAIN DYNAMICS; MODEL-FREE APPROACH; NMR-SPECTROSCOPY; RELAXATION; C-13; MACROMOLECULES; STRATEGIES; MOTIONS},
   language = {eng},
   issn = {0021-9606},
   journal = {Journal of Chemical Physics},
   title = {Understanding the methyl-TROSY effect over a wide range of magnetic fields},
   url = {https://aip.scitation.org/doi/10.1063/1.5095757},
   volume = {150},
   year = {2019}
}

TY  - JOUR
ID  - 1556101
AU  - Bolik-Coulon, N. - Cousin, A.F. - Kadeřávek, Pavel - Dumez, J.N. - Ferrage, F.
PY  - 2019
TI  - Understanding the methyl-TROSY effect over a wide range of magnetic fields
JF  - Journal of Chemical Physics
VL  - 150
IS  - 22
SP  - 224202
EP  - 224202
PB  - American Institute of Physics
SN  - 00219606
KW  - MOLECULAR-WEIGHT PROTEINS
KW  - SIDE-CHAIN DYNAMICS
KW  - MODEL-FREE APPROACH
KW  - NMR-SPECTROSCOPY
KW  - RELAXATION
KW  - C-13
KW  - MACROMOLECULES
KW  - STRATEGIES
KW  - MOTIONS
UR  - https://aip.scitation.org/doi/10.1063/1.5095757
L2  - https://aip.scitation.org/doi/10.1063/1.5095757
N2  - The use of relaxation interference in the methyl Transverse Relaxation-Optimized SpectroscopY (TROSY) experiment has opened new avenues for the study of large proteins and protein assemblies in nuclear magnetic resonance. So far, the theoretical description of the methyl-TROSY experiment has been limited to the slow-tumbling approximation, which is correct for large proteins on high-field spectrometers. In a recent paper, favorable relaxation interference was observed in the methyl groups of a small protein at a magnetic field as low as 0.33 T, well outside the slow-tumbling regime. Here, we present a model to describe relaxation interference in methyl groups over a broad range of magnetic fields, not limited to the slow-tumbling regime. We predict that the type of multiple-quantum transition that shows favorable relaxation properties change with the magnetic field. Under the condition of fast methyl-group rotation, methyl-TROSY experiments can be recorded over the entire range of magnetic fields from a fraction of 1 T up to 100 T.
ER  -

BOLIK-COULON, N., A.F. COUSIN, Pavel KADEŘÁVEK, J.N. DUMEZ and F. FERRAGE. Understanding the methyl-TROSY effect over a wide range of magnetic fields. \textit{Journal of Chemical Physics}. MELVILLE: American Institute of Physics, 2019, vol.~150, No~22, p.~224202-224213. ISSN~0021-9606. Available from: https://dx.doi.org/10.1063/1.5095757.

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