Need for novel detection targets in tuberculosis diagnostics of historical specimens - bioinformatic analysis

CHOCHOLOVÁ, Eva, Eva DROZDOVÁ, Dana FIALOVÁ, Kristýna BRZOBOHATÁ and Václav CHOCHOLA. Need for novel detection targets in tuberculosis diagnostics of historical specimens - bioinformatic analysis. In 6th International Anthropological Congress of Dr. Aleš Hrdlička. 2019. ISSN 2336-4777.

Basic information

• Original name: Need for novel detection targets in tuberculosis diagnostics of historical specimens - bioinformatic analysis
• Authors: CHOCHOLOVÁ, Eva (203 Czech Republic, guarantor, belonging to the institution), Eva DROZDOVÁ (203 Czech Republic, belonging to the institution), Dana FIALOVÁ (203 Czech Republic, belonging to the institution), Kristýna BRZOBOHATÁ (203 Czech Republic, belonging to the institution) and Václav CHOCHOLA (203 Czech Republic, belonging to the institution).
• Edition: 6th International Anthropological Congress of Dr. Aleš Hrdlička, 2019.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: Plný text
• RIV identification code: RIV/00216224:14310/19:00110709
• Organization unit: Faculty of Science
• ISSN: 2336-4777
• Keywords (in Czech): patogen; starobylá DNA; aDNA; tuberkulóza; MTBC; IS6110
• Keywords in English: pathogen; ancient DNA; aDNA; tuberculosis; MTBC; IS6110
• Tags: International impact, Reviewed

Abstract

Diagnosis of tuberculosis from historical specimens has been mostly based on detection of IS6110 by PCR and following gel electrophoresis. Even when some authors stressed the possibility that this insertion sequence may have similar counterparts in other microorganisms (e.g. soil bacteria), it continued to be used widely, sometimes without sequencing of the PCR product. This work is aiming at IS6110 and two other sequences that are potential targets for DNA detection of Mycobacteria tuberculosis complex. These three loci are compared by bioinformatic and phylogenetic analysis to assess their usability in ancient DNA studies.

Links

• MUNI/C/1717/2016, interní kód MU: Name: Identifikace bakteriálně přenosných nemocí z historického zubního kamene

Investor: Masaryk University, Rector's Program