Other formats:
BibTeX
LaTeX
RIS
@proceedings{1558736, author = {Chocholová, Eva and Fialová, Dana and Drozdová, Eva and Skoupý, Radim and Brzobohatá, Kristýna and Zwinsová, Barbora and Vídeňská, Petra and Chochola, Václav}, booktitle = {6th International Anthropological Congress of Dr. Aleš Hrdlička}, keywords = {ancient DNA; aDNA; SEM; dental calculus; metagenomics; contamination}, language = {eng}, title = {Microbial Contamination of Ancient Human Dental Calculus Studied by Scanning Electron Microscopy and Metagenomic Approach}, url = {http://www.pojivo.cz/pu/PU-2019-Suppl-2--Humpolec-190830.pdf}, year = {2019} }
TY - CONF ID - 1558736 AU - Chocholová, Eva - Fialová, Dana - Drozdová, Eva - Skoupý, Radim - Brzobohatá, Kristýna - Zwinsová, Barbora - Vídeňská, Petra - Chochola, Václav PY - 2019 TI - Microbial Contamination of Ancient Human Dental Calculus Studied by Scanning Electron Microscopy and Metagenomic Approach KW - ancient DNA KW - aDNA KW - SEM KW - dental calculus KW - metagenomics KW - contamination UR - http://www.pojivo.cz/pu/PU-2019-Suppl-2--Humpolec-190830.pdf L2 - http://www.pojivo.cz/pu/PU-2019-Suppl-2--Humpolec-190830.pdf N2 - Dental calculus has great potential in the research of oral microbiome in past populations. Alongside the advantages of novel molecular approaches applied on ancient human dental calculus, we encounter the drawbacks as well. One of the main issues is contamination. There is a possibility of detection of microbial contamination by scanning electron microscopy prior to subsequent expensive molecular analysis. Although decontamination is always part of the workflow, the metagenomic analysis points out the residual microbial contamination. This study presents the means of detecting contamination and precautions to be taken for the best prevention of it. ER -
CHOCHOLOVÁ, Eva, Dana FIALOVÁ, Eva DROZDOVÁ, Radim SKOUPÝ, Kristýna BRZOBOHATÁ, Barbora ZWINSOVÁ, Petra VÍDEŇSKÁ and Václav CHOCHOLA. Microbial Contamination of Ancient Human Dental Calculus Studied by Scanning Electron Microscopy and Metagenomic Approach. In \textit{6th International Anthropological Congress of Dr. Aleš Hrdlička}. 2019. ISSN~2336-4777.
|