Microbial Contamination of Ancient Human Dental Calculus Studied by Scanning Electron Microscopy and Metagenomic Approach

CHOCHOLOVÁ, Eva, Dana FIALOVÁ, Eva DROZDOVÁ, Radim SKOUPÝ, Kristýna BRZOBOHATÁ, Barbora ZWINSOVÁ, Petra VÍDEŇSKÁ and Václav CHOCHOLA. Microbial Contamination of Ancient Human Dental Calculus Studied by Scanning Electron Microscopy and Metagenomic Approach. In 6th International Anthropological Congress of Dr. Aleš Hrdlička. 2019. ISSN 2336-4777.

Basic information

• Original name: Microbial Contamination of Ancient Human Dental Calculus Studied by Scanning Electron Microscopy and Metagenomic Approach
• Authors: CHOCHOLOVÁ, Eva (203 Czech Republic, guarantor, belonging to the institution), Dana FIALOVÁ (203 Czech Republic, belonging to the institution), Eva DROZDOVÁ (203 Czech Republic, belonging to the institution), Radim SKOUPÝ (203 Czech Republic), Kristýna BRZOBOHATÁ (203 Czech Republic, belonging to the institution), Barbora ZWINSOVÁ (203 Czech Republic, belonging to the institution), Petra VÍDEŇSKÁ (203 Czech Republic, belonging to the institution) and Václav CHOCHOLA (203 Czech Republic, belonging to the institution).
• Edition: 6th International Anthropological Congress of Dr. Aleš Hrdlička, 2019.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: Plný text
• RIV identification code: RIV/00216224:14310/19:00110713
• Organization unit: Faculty of Science
• ISSN: 2336-4777
• Keywords (in Czech): starobylá DNA; aDNA; SEM; zubní kámen; metagenomika; kontaminace
• Keywords in English: ancient DNA; aDNA; SEM; dental calculus; metagenomics; contamination
• Tags: International impact, Reviewed

Abstract

Dental calculus has great potential in the research of oral microbiome in past populations. Alongside the advantages of novel molecular approaches applied on ancient human dental calculus, we encounter the drawbacks as well. One of the main issues is contamination. There is a possibility of detection of microbial contamination by scanning electron microscopy prior to subsequent expensive molecular analysis. Although decontamination is always part of the workflow, the metagenomic analysis points out the residual microbial contamination. This study presents the means of detecting contamination and precautions to be taken for the best prevention of it.

Links

• MUNI/C/1717/2016, interní kód MU: Name: Identifikace bakteriálně přenosných nemocí z historického zubního kamene

Investor: Masaryk University, Rector's Program