Microbial Contamination of Ancient Human Dental Calculus
Studied by Scanning Electron Microscopy and Metagenomic
Approach

a 2019

Microbial Contamination of Ancient Human Dental Calculus Studied by Scanning Electron Microscopy and Metagenomic Approach

CHOCHOLOVÁ, Eva, Dana FIALOVÁ, Eva DROZDOVÁ, Radim SKOUPÝ, Kristýna BRZOBOHATÁ et. al.

Basic information

Original name

Microbial Contamination of Ancient Human Dental Calculus Studied by Scanning Electron Microscopy and Metagenomic Approach

Authors

CHOCHOLOVÁ, Eva (203 Czech Republic, guarantor, belonging to the institution), Dana FIALOVÁ (203 Czech Republic, belonging to the institution), Eva DROZDOVÁ (203 Czech Republic, belonging to the institution), Radim SKOUPÝ (203 Czech Republic), Kristýna BRZOBOHATÁ (203 Czech Republic, belonging to the institution), Barbora ZWINSOVÁ (203 Czech Republic, belonging to the institution), Petra VÍDEŇSKÁ (203 Czech Republic, belonging to the institution) and Václav CHOCHOLA (203 Czech Republic, belonging to the institution)

Edition

6th International Anthropological Congress of Dr. Aleš Hrdlička, 2019

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Plný text

RIV identification code

RIV/00216224:14310/19:00110713

Organization unit

Faculty of Science

ISSN

Keywords (in Czech)

starobylá DNA; aDNA; SEM; zubní kámen; metagenomika; kontaminace

Keywords in English

ancient DNA; aDNA; SEM; dental calculus; metagenomics; contamination

Abstract

V originále

Dental calculus has great potential in the research of oral microbiome in past populations. Alongside the advantages of novel molecular approaches applied on ancient human dental calculus, we encounter the drawbacks as well. One of the main issues is contamination. There is a possibility of detection of microbial contamination by scanning electron microscopy prior to subsequent expensive molecular analysis. Although decontamination is always part of the workflow, the metagenomic analysis points out the residual microbial contamination. This study presents the means of detecting contamination and precautions to be taken for the best prevention of it.

Links

MUNI/C/1717/2016, interní kód MU

Name: Identifikace bakteriálně přenosných nemocí z historického zubního kamene

Investor: Masaryk University, Rector's Program

Citovat

CHOCHOLOVÁ, Eva, Dana FIALOVÁ, Eva DROZDOVÁ, Radim SKOUPÝ, Kristýna BRZOBOHATÁ, Barbora ZWINSOVÁ, Petra VÍDEŇSKÁ and Václav CHOCHOLA. Microbial Contamination of Ancient Human Dental Calculus Studied by Scanning Electron Microscopy and Metagenomic Approach. In 6th International Anthropological Congress of Dr. Aleš Hrdlička. 2019. ISSN 2336-4777.

@proceedings{1558736,
   author = {Chocholová, Eva and Fialová, Dana and Drozdová, Eva and Skoupý, Radim and Brzobohatá, Kristýna and Zwinsová, Barbora and Vídeňská, Petra and Chochola, Václav},
   booktitle = {6th International Anthropological Congress of Dr. Aleš Hrdlička},
   keywords = {ancient DNA; aDNA; SEM; dental calculus; metagenomics; contamination},
   language = {eng},
   title = {Microbial Contamination of Ancient Human Dental Calculus Studied by Scanning Electron Microscopy and Metagenomic Approach},
   url = {http://www.pojivo.cz/pu/PU-2019-Suppl-2--Humpolec-190830.pdf},
   year = {2019}
}

TY  - CONF
ID  - 1558736
AU  - Chocholová, Eva - Fialová, Dana - Drozdová, Eva - Skoupý, Radim - Brzobohatá, Kristýna - Zwinsová, Barbora - Vídeňská, Petra - Chochola, Václav
PY  - 2019
TI  - Microbial Contamination of Ancient Human Dental Calculus Studied by Scanning Electron Microscopy and Metagenomic Approach
KW  - ancient DNA
KW  - aDNA
KW  - SEM
KW  - dental calculus
KW  - metagenomics
KW  - contamination
UR  - http://www.pojivo.cz/pu/PU-2019-Suppl-2--Humpolec-190830.pdf
L2  - http://www.pojivo.cz/pu/PU-2019-Suppl-2--Humpolec-190830.pdf
N2  - Dental calculus has great potential in the research of oral microbiome in past populations. Alongside the advantages of novel molecular approaches applied on ancient human dental calculus, we encounter the drawbacks as well. One of the main issues is contamination. There is a possibility of detection of microbial contamination by scanning electron microscopy prior to subsequent expensive molecular analysis. Although decontamination is always part of the workflow, the metagenomic analysis points out the residual microbial contamination. This study presents the means of detecting contamination and precautions to be taken for the best prevention of it.
ER  -

CHOCHOLOVÁ, Eva, Dana FIALOVÁ, Eva DROZDOVÁ, Radim SKOUPÝ, Kristýna BRZOBOHATÁ, Barbora ZWINSOVÁ, Petra VÍDEŇSKÁ and Václav CHOCHOLA. Microbial Contamination of Ancient Human Dental Calculus Studied by Scanning Electron Microscopy and Metagenomic Approach. In \textit{6th International Anthropological Congress of Dr. Aleš Hrdlička}. 2019. ISSN~2336-4777.

Detailed Information on Publication Record