Differentiation of neural rosettes from human pluripotent stem
cells in vitro is sequentially regulated on a molecular level
and accomplished by the mechanism reminiscent of secondary
neurulation

J 2019

Differentiation of neural rosettes from human pluripotent stem cells in vitro is sequentially regulated on a molecular level and accomplished by the mechanism reminiscent of secondary neurulation

FEDOROVÁ, Veronika, Tereza VÁŇOVÁ, Lina Mohamed Bahaael ELREFAE, Jakub POSPÍŠIL, Martina PETRÁŠOVÁ et. al.

Basic information

Original name

Differentiation of neural rosettes from human pluripotent stem cells in vitro is sequentially regulated on a molecular level and accomplished by the mechanism reminiscent of secondary neurulation

Authors

FEDOROVÁ, Veronika (203 Czech Republic, belonging to the institution), Tereza VÁŇOVÁ (203 Czech Republic, belonging to the institution), Lina Mohamed Bahaael ELREFAE (818 Egypt, belonging to the institution), Jakub POSPÍŠIL (203 Czech Republic, belonging to the institution), Martina PETRÁŠOVÁ (703 Slovakia, belonging to the institution), Veronika KOLAJOVÁ (203 Czech Republic, belonging to the institution), Zuzana HUDACOVA (203 Czech Republic), Jana BANIARIOVÁ (703 Slovakia, belonging to the institution), Martin BARÁK (203 Czech Republic, belonging to the institution), Lucie PEŠKOVÁ (203 Czech Republic, belonging to the institution), Tomáš BÁRTA (203 Czech Republic, belonging to the institution), Markéta KAUCKÁ (203 Czech Republic), Michael KILLINGER (203 Czech Republic), Josef VEČEŘA (203 Czech Republic, belonging to the institution), Ondřej BERNATÍK (203 Czech Republic, belonging to the institution), Lukáš ČAJÁNEK (203 Czech Republic, belonging to the institution), Hana HŘÍBKOVÁ (203 Czech Republic, belonging to the institution) and Dáša BOHAČIAKOVÁ (703 Slovakia, guarantor, belonging to the institution)

Edition

Stem Cell Research, Amsterdam, Elsevier Science BV, 2019, 1873-5061

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.495

RIV identification code

RIV/00216224:14110/19:00107663

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.scr.2019.101563

UT WoS

000491224500006

Keywords in English

BMP; Differentiation; Human embryonic stem cells; Induced pluripotent stem cells; Neural rosettes; Secondary neurulation

Abstract

V originále

Development of neural tube has been extensively modeled in vitro using human pluripotent stem cells (hPSCs) that are able to form radially organized cellular structures called neural rosettes. While a great amount of research has been done using neural rosettes, studies have only inadequately addressed how rosettes are formed and what the molecular mechanisms and pathways involved in their formation are. Here we address this question by detailed analysis of the expression of pluripotency and differentiation-associated proteins during the early onset of differentiation of hPSCs towards neural rosettes. Additionally, we show that the BMP signaling is likely contributing to the formation of the complex cluster of neural rosettes and its inhibition leads to the altered expression of PAX6, SOX2 and SOX1 proteins and the rosette morphology. Finally, we provide evidence that the mechanism of neural rosettes formation in vitro is reminiscent of the process of secondary neurulation rather than that of primary neurulation in vivo. Since secondary neurulation is a largely unexplored process, its understanding will ultimately assist the development of methods to prevent caudal neural tube defects in humans.