Telomerase RNAs in land plants

J 2019

Telomerase RNAs in land plants

FAJKUS, Petr, Vratislav PEŠKA, Michal ZÁVODNÍK, Miloslava FOJTOVÁ, Jana FULNEČKOVÁ et. al.

Basic information

Original name

Telomerase RNAs in land plants

Authors

FAJKUS, Petr (203 Czech Republic, belonging to the institution), Vratislav PEŠKA (203 Czech Republic), Michal ZÁVODNÍK (203 Czech Republic, belonging to the institution), Miloslava FOJTOVÁ (203 Czech Republic, belonging to the institution), Jana FULNEČKOVÁ (203 Czech Republic, belonging to the institution), Šimon DOBIAS (703 Slovakia, belonging to the institution), Agata Magdalena KILAR (616 Poland, belonging to the institution), Martina DVOŘÁČKOVÁ (203 Czech Republic, belonging to the institution), Dagmar ZACHOVÁ (203 Czech Republic, belonging to the institution), Ivona NEČASOVÁ (203 Czech Republic, belonging to the institution), Jason SIMS (40 Austria), Eva SÝKOROVÁ (203 Czech Republic) and Jiří FAJKUS (203 Czech Republic, guarantor, belonging to the institution)

Edition

Nucleic acids research, Oxford, Oxford University Press, 2019, 0305-1048

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Webová stránka článku v časopise NAR

Impact factor

Impact factor: 11.502

RIV identification code

RIV/00216224:14740/19:00107675

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1093/nar/gkz695

UT WoS

000491241400037

Keywords in English

Telomerase RNA subunit; plant

Abstract

V originále

To elucidate the molecular nature of evolutionary changes of telomeres in the plant order Asparagales, we aimed to characterize telomerase RNA subunits (TRs) in these plants. The unusually long telomere repeat unit in Allium plants (12 nt) allowed us to identify TRs in transcriptomic data of representative species of the Allium genus. Orthologous TRs were then identified in Asparagales plants harbouring telomere DNA composed of TTAGGG (human type) or TTTAGGG (Arabidopsis-type) repeats. Further, we identified TRs across the land plant phylogeny, including common model plants, crop plants, and plants with unusual telomeres. Several lines of functional testing demonstrate the templating telomerase function of the identified TRs and disprove a functionality of the only previously reported plant telomerase RNA in Arabidopsis thaliana. Importantly, our results change the existing paradigm in plant telomere biology which has been based on the existence of a relatively conserved telomerase reverse transcriptase subunit (TERT) associating with highly divergent TRs even between closely related plant taxa. The finding of a monophyletic origin of genuine TRs across land plants opens the possibility to identify TRs directly in transcriptomic or genomic data and/or predict telomere sequences synthesized according to the respective TR template region.

Links

ATCZ40, interní kód MU

Name: Creating synergies between research infrastructures to stimulate innovation in the cross-border region (Acronym: RIAT-CZ)

Investor: Ministry for Regional Development of the CR

GA17-09644S, research and development project

Name: Molekulární podstata evolučních přeměn telomer u rostlin řádu Asparagales

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

90062, large research infrastructures

Name: Czech-BioImaging

90091, large research infrastructures

Name: NCMG

Citovat

FAJKUS, Petr, Vratislav PEŠKA, Michal ZÁVODNÍK, Miloslava FOJTOVÁ, Jana FULNEČKOVÁ, Šimon DOBIAS, Agata Magdalena KILAR, Martina DVOŘÁČKOVÁ, Dagmar ZACHOVÁ, Ivona NEČASOVÁ, Jason SIMS, Eva SÝKOROVÁ and Jiří FAJKUS. Telomerase RNAs in land plants. Nucleic acids research. Oxford: Oxford University Press, 2019, vol. 47, No 18, p. 9842-9856. ISSN 0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkz695.

@article{1561456,
   author = {Fajkus, Petr and Peška, Vratislav and Závodník, Michal and Fojtová, Miloslava and Fulnečková, Jana and Dobias, Šimon and Kilar, Agata Magdalena and Dvořáčková, Martina and Zachová, Dagmar and Nečasová, Ivona and Sims, Jason and Sýkorová, Eva and Fajkus, Jiří},
   article_location = {Oxford},
   article_number = {18},
   doi = {http://dx.doi.org/10.1093/nar/gkz695},
   keywords = {Telomerase RNA subunit; plant},
   language = {eng},
   issn = {0305-1048},
   journal = {Nucleic acids research},
   title = {Telomerase RNAs in land plants},
   url = {https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkz695/5545009},
   volume = {47},
   year = {2019}
}

TY  - JOUR
ID  - 1561456
AU  - Fajkus, Petr - Peška, Vratislav - Závodník, Michal - Fojtová, Miloslava - Fulnečková, Jana - Dobias, Šimon - Kilar, Agata Magdalena - Dvořáčková, Martina - Zachová, Dagmar - Nečasová, Ivona - Sims, Jason - Sýkorová, Eva - Fajkus, Jiří
PY  - 2019
TI  - Telomerase RNAs in land plants
JF  - Nucleic acids research
VL  - 47
IS  - 18
SP  - 9842-9856
EP  - 9842-9856
PB  - Oxford University Press
SN  - 03051048
KW  - Telomerase RNA subunit
KW  - plant
UR  - https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkz695/5545009
L2  - https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkz695/5545009
N2  - To elucidate the molecular nature of evolutionary changes of telomeres in the plant order Asparagales, we aimed to characterize telomerase RNA subunits (TRs) in these plants. The unusually long telomere repeat unit in Allium plants (12 nt) allowed us to identify TRs in transcriptomic data of representative species of the Allium genus. Orthologous TRs were then identified in Asparagales plants harbouring telomere DNA composed of TTAGGG (human type) or TTTAGGG (Arabidopsis-type) repeats. Further, we identified TRs across the land plant phylogeny, including common model plants, crop plants, and plants with unusual telomeres. Several lines of functional testing demonstrate the templating telomerase function of the identified TRs and disprove a functionality of the only previously reported plant telomerase RNA in Arabidopsis thaliana. Importantly, our results change the existing paradigm in plant telomere biology which has been based on the existence of a relatively conserved telomerase reverse transcriptase subunit (TERT) associating with highly divergent TRs even between closely related plant taxa. The finding of a monophyletic origin of genuine TRs across land plants opens the possibility to identify TRs directly in transcriptomic or genomic data and/or predict telomere sequences synthesized according to the respective TR template region.
ER  -

FAJKUS, Petr, Vratislav PEŠKA, Michal ZÁVODNÍK, Miloslava FOJTOVÁ, Jana FULNEČKOVÁ, Šimon DOBIAS, Agata Magdalena KILAR, Martina DVOŘÁČKOVÁ, Dagmar ZACHOVÁ, Ivona NEČASOVÁ, Jason SIMS, Eva SÝKOROVÁ and Jiří FAJKUS. Telomerase RNAs in land plants. \textit{Nucleic acids research}. Oxford: Oxford University Press, 2019, vol.~47, No~18, p.~9842-9856. ISSN~0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkz695.

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