WILLEMSEN, Joschka, Oliver WICHT, Julia WOLANSKI, Nina BAUR, Sandra BASTIAN, Darya HAAS, Petr MATULA, Bettina KNAPP, Laurene MEYNIEL-SCHICKLIN, Chen WANG, Ralf BARTENSCHLAGER, Volker LOHMANN, Karl ROHR, Holger ERFLE, Lars KADERALI, Joseph MARCOTRIGIANO, Andreas PICHLMAIR and Marco BINDER. Phosphorylation-Dependent Feedback Inhibition of RIG-I by DAPK1 Identified by Kinome-wide siRNA Screening. Molecular Cell, CAMBRIDGE: CELL PRESS, 2017, vol. 65, No 3, p. "403"-"415.e8". ISSN 1097-2765. doi:10.1016/j.molcel.2016.12.021.
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Basic information
Original name Phosphorylation-Dependent Feedback Inhibition of RIG-I by DAPK1 Identified by Kinome-wide siRNA Screening
Authors WILLEMSEN, Joschka, Oliver WICHT, Julia WOLANSKI, Nina BAUR, Sandra BASTIAN, Darya HAAS, Petr MATULA (203 Czech Republic, guarantor, belonging to the institution), Bettina KNAPP, Laurene MEYNIEL-SCHICKLIN, Chen WANG, Ralf BARTENSCHLAGER, Volker LOHMANN, Karl ROHR, Holger ERFLE, Lars KADERALI, Joseph MARCOTRIGIANO, Andreas PICHLMAIR and Marco BINDER.
Edition Molecular Cell, CAMBRIDGE, CELL PRESS, 2017, 1097-2765.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10601 Cell biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 14.248
RIV identification code RIV/00216224:14330/17:00107683
Organization unit Faculty of Informatics
Doi http://dx.doi.org/10.1016/j.molcel.2016.12.021
UT WoS 000396431400007
Keywords in English innate immunity; antiviral response; pattern recognition receptors; signal transduction; feedback regulation; interferon system; cytokines; DAPK1; RIG-I; DDX58
Tags International impact, Reviewed
Changed by Changed by: RNDr. Pavel Šmerk, Ph.D., učo 3880. Changed: 28/4/2020 07:48.
Abstract
Cell-autonomous induction of type I interferon must be stringently regulated. Rapid induction is key to control virus infection, whereas proper limitation of signaling is essential to prevent immunopathology and autoimmune disease. Using unbiased kinome-wide RNAi screening followed by thorough validation, we identified 22 factors that regulate RIG-I/IRF3 signaling activity. We describe a negative-feedback mechanism targeting RIG-I activity, which is mediated by death associated protein kinase 1 (DAPK1). RIG-I signaling triggers DAPK1 kinase activation, and active DAPK1 potently inhibits RIG-I stimulated IRF3 activity and interferon-beta production. DAPK1 phosphorylates RIG-I in vitro at previously reported as well as other sites that limit 5'ppp-dsRNA sensing and virtually abrogate RIG-I activation.
Links
GBP302/12/G157, research and development projectName: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii
Investor: Czech Science Foundation, Projects to promote excellence in basic research
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