| WILLEMSEN, Joschka, Oliver WICHT, Julia WOLANSKI, Nina BAUR, Sandra BASTIAN, Darya HAAS, Petr MATULA, Bettina KNAPP, Laurene MEYNIEL-SCHICKLIN, Chen WANG, Ralf BARTENSCHLAGER, Volker LOHMANN, Karl ROHR, Holger ERFLE, Lars KADERALI, Joseph MARCOTRIGIANO, Andreas PICHLMAIR and Marco BINDER. Phosphorylation-Dependent Feedback Inhibition of RIG-I by DAPK1 Identified by Kinome-wide siRNA Screening. Molecular Cell. CAMBRIDGE: CELL PRESS, 2017, vol. 65, No 3, p. "403"-"415.e8", 21 pp. ISSN 1097-2765. Available from: https://dx.doi.org/10.1016/j.molcel.2016.12.021. |
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@article{1563380,
author = {Willemsen, Joschka and Wicht, Oliver and Wolanski, Julia and Baur, Nina and Bastian, Sandra and Haas, Darya and Matula, Petr and Knapp, Bettina and MeynielandSchicklin, Laurene and Wang, Chen and Bartenschlager, Ralf and Lohmann, Volker and Rohr, Karl and Erfle, Holger and Kaderali, Lars and Marcotrigiano, Joseph and Pichlmair, Andreas and Binder, Marco},
article_location = {CAMBRIDGE},
article_number = {3},
doi = {http://dx.doi.org/10.1016/j.molcel.2016.12.021},
keywords = {innate immunity; antiviral response; pattern recognition receptors; signal transduction; feedback regulation; interferon system; cytokines; DAPK1; RIG-I; DDX58},
language = {eng},
issn = {1097-2765},
journal = {Molecular Cell},
title = {Phosphorylation-Dependent Feedback Inhibition of RIG-I by DAPK1 Identified by Kinome-wide siRNA Screening},
url = {http://dx.doi.org/10.1016/j.molcel.2016.12.021},
volume = {65},
year = {2017}
}
TY - JOUR
ID - 1563380
AU - Willemsen, Joschka - Wicht, Oliver - Wolanski, Julia - Baur, Nina - Bastian, Sandra - Haas, Darya - Matula, Petr - Knapp, Bettina - Meyniel-Schicklin, Laurene - Wang, Chen - Bartenschlager, Ralf - Lohmann, Volker - Rohr, Karl - Erfle, Holger - Kaderali, Lars - Marcotrigiano, Joseph - Pichlmair, Andreas - Binder, Marco
PY - 2017
TI - Phosphorylation-Dependent Feedback Inhibition of RIG-I by DAPK1 Identified by Kinome-wide siRNA Screening
JF - Molecular Cell
VL - 65
IS - 3
SP - "403"-"415.e8"
EP - "403"-"415.e8"
PB - CELL PRESS
SN - 10972765
KW - innate immunity
KW - antiviral response
KW - pattern recognition receptors
KW - signal transduction
KW - feedback regulation
KW - interferon system
KW - cytokines
KW - DAPK1
KW - RIG-I
KW - DDX58
UR - http://dx.doi.org/10.1016/j.molcel.2016.12.021
L2 - http://dx.doi.org/10.1016/j.molcel.2016.12.021
N2 - Cell-autonomous induction of type I interferon must be stringently regulated. Rapid induction is key to control virus infection, whereas proper limitation of signaling is essential to prevent immunopathology and autoimmune disease. Using unbiased kinome-wide RNAi screening followed by thorough validation, we identified 22 factors that regulate RIG-I/IRF3 signaling activity. We describe a negative-feedback mechanism targeting RIG-I activity, which is mediated by death associated protein kinase 1 (DAPK1). RIG-I signaling triggers DAPK1 kinase activation, and active DAPK1 potently inhibits RIG-I stimulated IRF3 activity and interferon-beta production. DAPK1 phosphorylates RIG-I in vitro at previously reported as well as other sites that limit 5'ppp-dsRNA sensing and virtually abrogate RIG-I activation.
ER -
WILLEMSEN, Joschka, Oliver WICHT, Julia WOLANSKI, Nina BAUR, Sandra BASTIAN, Darya HAAS, Petr MATULA, Bettina KNAPP, Laurene MEYNIEL-SCHICKLIN, Chen WANG, Ralf BARTENSCHLAGER, Volker LOHMANN, Karl ROHR, Holger ERFLE, Lars KADERALI, Joseph MARCOTRIGIANO, Andreas PICHLMAIR and Marco BINDER. Phosphorylation-Dependent Feedback Inhibition of RIG-I by DAPK1 Identified by Kinome-wide siRNA Screening. \textit{Molecular Cell}. CAMBRIDGE: CELL PRESS, 2017, vol.~65, No~3, p.~''403''-''415.e8'', 21 pp. ISSN~1097-2765. Available from: https://dx.doi.org/10.1016/j.molcel.2016.12.021.
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