What can egg-proteases tell us about the pathogenesis caused by trematodes?

RESLOVÁ, Nikol, Lucie ŠKORPÍKOVÁ, Jana ILGOVÁ, Pavel ROUDNICKÝ, Jiří VOREL and Martin KAŠNÝ. What can egg-proteases tell us about the pathogenesis caused by trematodes? In JOINT COMBAR –ACSRPC MEETING: Anthelmintic resistance in ruminants:Who cares? 2019.

Basic information

• Original name: What can egg-proteases tell us about the pathogenesis caused by trematodes?
• Authors: RESLOVÁ, Nikol, Lucie ŠKORPÍKOVÁ, Jana ILGOVÁ, Pavel ROUDNICKÝ, Jiří VOREL and Martin KAŠNÝ.
• Edition: JOINT COMBAR –ACSRPC MEETING: Anthelmintic resistance in ruminants:Who cares? 2019.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Country of publisher: Belgium
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Science
• Keywords (in Czech): Fasciola hepatica, Schistosoma mansoni, exkrečně-sekreční produkty ve vajíčkách, proteázy
• Keywords in English: Fasciola hepatica, Schistosoma mansoni, excretory-secretory products of eggs, proteases

Abstract

Eggs released by female schistosomes are considered to be the primary cause of symptoms connected with schistosomiasis. Discharge of molecules (mainly proteases) by eggs facilitate their migration through the tissue of the host. Nevertheless, as a side-effect they cause severe pathogenesis manifested by inflammation, granuloma formation and subsequent fibrosis of the tissue. The eggs of Fasciola hepatica, trematode which inhabits the bile ducts of number of ruminants and other vertebrate species, are not supposed to have such detrimental effects on its host although the eggs related proteolytic activity was recorded. The detailed protein composition of F. hepaticaeggs ́ products remains unrevealed. Comparison of proteins from the excretory-secretory products of eggs from highly pathogenic vs. non-pathogenic species could represent a step towards better understanding of biology of F. hepatica, pathogenesis associated with S. mansoni infections and thus contribute to effective drug treatment. In our newly launched scientific project we aim to compare proteolytic cocktails present in E/S products ofS.mansoniand F. hepatica eggs. We focus particularly on the identification of proteases by functional proteomics, evaluation of their physiological role by genomic studies, and their recombinant expression and biochemical characterization.

Links

• GA19-17269S, research and development project: Name: Proteolýza ve vejcích parazitických červů: úloha v patologii a její regulace (Acronym: Proteolýza vajec červů)

Investor: Czech Science Foundation

• MUNI/A/0918/2018, interní kód MU: Name: Studium evolučních a ekologických vztahů a procesů u akvatických bezobratlých živočichů a v hostitelsko-parazitických systémech (Acronym: EvolEcolParaHydro)

Investor: Masaryk University, Category A