The serum protease network-one key to understand complex
regional pain syndrome pathophysiology

J 2019

The serum protease network-one key to understand complex regional pain syndrome pathophysiology

KONIG, Simone, Malte BAYER, Violeta DIMOVA, Myriam HERRNBERGER, Fabiola ESCOLANO-LOZANO et. al.

Basic information

Original name

The serum protease network-one key to understand complex regional pain syndrome pathophysiology

Authors

KONIG, Simone (276 Germany), Malte BAYER (276 Germany), Violeta DIMOVA (276 Germany), Myriam HERRNBERGER (276 Germany), Fabiola ESCOLANO-LOZANO (276 Germany), Josef BEDNAŘÍK (203 Czech Republic, guarantor, belonging to the institution), Eva VLČKOVÁ (203 Czech Republic, belonging to the institution), Heike RITTNER (276 Germany), Tanja SCHLERETH (276 Germany) and Frank BIRKLEIN (276 Germany)

Edition

Pain : the journal of the international association for the study of pain. New York, Elsevier, 2019, 0304-3959

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30103 Neurosciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.483

RIV identification code

RIV/00216224:14110/19:00110899

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1097/j.pain.0000000000001503

UT WoS

000480762400015

Keywords in English

Complex regional pain syndrome; Chronic pain; Bradykinin; Serum peptidase

Abstract

V originále

Complex regional pain syndrome (CRPS) develops after fracture. The acute CRPS phenotype resembles exaggerated inflammation, which is explained by local and systemic activation of a proinflammatory network including peptides and cytokines. Epidemiologic data suggest that inactivation of the peptidase angiotensin-converting enzyme in patients treated for hypertension increases the odds to develop CRPS. This hint leads us to investigate the serum protease network activity in patients with CRPS vs respective controls. For this purpose, we developed a dabsyl-bradykinin (DBK)-based assay and used it to investigate patients with CRPS, as well as healthy and pain (painful diabetic neuropathy [dPNP]) controls. The major result is that the degradation of DBK to fragments 1-8 and 1-5 in healthy control and dPNP is shifted to higher values for DBK1-8 and lower values for DBK1-5 at 1 hour of incubation in patients with CRPS. Using this novel reporter peptide assay, we have been able to show that the resolving protease network for mediators such as BK might be different in patients with CRPS; having a look at the clinical signs, which resemble inflammation, this resolving protease network is probably less effective in CRPS.

Links

MUNI/A/1419/2018, interní kód MU

Name: Diagnostika a patofyziologie neuropatické bolesti (Acronym: PNB)

Investor: Masaryk University, Category A

602133, interní kód MU

Name: ncRNAPain - Non-coding RNAs in neurogenic and neuropathic pain mechanisms and their application for risk assessment, patient stratification and personalised pain medicine (Acronym: ncRNAPain)

Investor: European Union, Cooperation

Citovat

KONIG, Simone, Malte BAYER, Violeta DIMOVA, Myriam HERRNBERGER, Fabiola ESCOLANO-LOZANO, Josef BEDNAŘÍK, Eva VLČKOVÁ, Heike RITTNER, Tanja SCHLERETH and Frank BIRKLEIN. The serum protease network-one key to understand complex regional pain syndrome pathophysiology. Pain : the journal of the international association for the study of pain. New York: Elsevier, 2019, vol. 160, No 6, p. 1402-1409. ISSN 0304-3959. Available from: https://dx.doi.org/10.1097/j.pain.0000000000001503.

@article{1567478,
   author = {Konig, Simone and Bayer, Malte and Dimova, Violeta and Herrnberger, Myriam and EscolanoandLozano, Fabiola and Bednařík, Josef and Vlčková, Eva and Rittner, Heike and Schlereth, Tanja and Birklein, Frank},
   article_location = {New York},
   article_number = {6},
   doi = {http://dx.doi.org/10.1097/j.pain.0000000000001503},
   keywords = {Complex regional pain syndrome; Chronic pain; Bradykinin; Serum peptidase},
   language = {eng},
   issn = {0304-3959},
   journal = {Pain : the journal of the international association for the study of pain.},
   title = {The serum protease network-one key to understand complex regional pain syndrome pathophysiology},
   url = {http://dx.doi.org/10.1097/j.pain.0000000000001503},
   volume = {160},
   year = {2019}
}

TY  - JOUR
ID  - 1567478
AU  - Konig, Simone - Bayer, Malte - Dimova, Violeta - Herrnberger, Myriam - Escolano-Lozano, Fabiola - Bednařík, Josef - Vlčková, Eva - Rittner, Heike - Schlereth, Tanja - Birklein, Frank
PY  - 2019
TI  - The serum protease network-one key to understand complex regional pain syndrome pathophysiology
JF  - Pain : the journal of the international association for the study of pain.
VL  - 160
IS  - 6
SP  - 1402-1409
EP  - 1402-1409
PB  - Elsevier
SN  - 03043959
KW  - Complex regional pain syndrome
KW  - Chronic pain
KW  - Bradykinin
KW  - Serum peptidase
UR  - http://dx.doi.org/10.1097/j.pain.0000000000001503
L2  - http://dx.doi.org/10.1097/j.pain.0000000000001503
N2  - Complex regional pain syndrome (CRPS) develops after fracture. The acute CRPS phenotype resembles exaggerated inflammation, which is explained by local and systemic activation of a proinflammatory network including peptides and cytokines. Epidemiologic data suggest that inactivation of the peptidase angiotensin-converting enzyme in patients treated for hypertension increases the odds to develop CRPS. This hint leads us to investigate the serum protease network activity in patients with CRPS vs respective controls. For this purpose, we developed a dabsyl-bradykinin (DBK)-based assay and used it to investigate patients with CRPS, as well as healthy and pain (painful diabetic neuropathy [dPNP]) controls. The major result is that the degradation of DBK to fragments 1-8 and 1-5 in healthy control and dPNP is shifted to higher values for DBK1-8 and lower values for DBK1-5 at 1 hour of incubation in patients with CRPS. Using this novel reporter peptide assay, we have been able to show that the resolving protease network for mediators such as BK might be different in patients with CRPS; having a look at the clinical signs, which resemble inflammation, this resolving protease network is probably less effective in CRPS.
ER  -

KONIG, Simone, Malte BAYER, Violeta DIMOVA, Myriam HERRNBERGER, Fabiola ESCOLANO-LOZANO, Josef BEDNAŘÍK, Eva VLČKOVÁ, Heike RITTNER, Tanja SCHLERETH and Frank BIRKLEIN. The serum protease network-one key to understand complex regional pain syndrome pathophysiology. \textit{Pain : the journal of the international association for the study of pain.}. New York: Elsevier, 2019, vol.~160, No~6, p.~1402-1409. ISSN~0304-3959. Available from: https://dx.doi.org/10.1097/j.pain.0000000000001503.

Detailed Information on Publication Record