| MICHÁLEK, Jan, Karel ŠTĚPKA, Michal KOZUBEK, Jarmila NAVRÁTILOVÁ, Barbora PAVLATOVSKÁ, Markéta MACHÁLKOVÁ, Jan PREISLER and Adam PRUŠKA. Quantitative Assessment of Anti-Cancer Drug Efficacy From Coregistered Mass Spectrometry and Fluorescence Microscopy Images of Multicellular Tumor Spheroids. Microscopy and Microanalysis. Cambridge University Press, 2019, vol. 25, No 6, p. 1311-1322. ISSN 1431-9276. Available from: https://dx.doi.org/10.1017/S1431927619014983. |
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@article{1567999,
author = {Michálek, Jan and Štěpka, Karel and Kozubek, Michal and Navrátilová, Jarmila and Pavlatovská, Barbora and Machálková, Markéta and Preisler, Jan and Pruška, Adam},
article_number = {6},
doi = {http://dx.doi.org/10.1017/S1431927619014983},
keywords = {confocal microscopy; image registration; MALDI MS; mass spectrometry imaging; peeling},
language = {eng},
issn = {1431-9276},
journal = {Microscopy and Microanalysis},
title = {Quantitative Assessment of Anti-Cancer Drug Efficacy From Coregistered Mass Spectrometry and Fluorescence Microscopy Images of Multicellular Tumor Spheroids},
url = {http://dx.doi.org/10.1017/S1431927619014983},
volume = {25},
year = {2019}
}
TY - JOUR
ID - 1567999
AU - Michálek, Jan - Štěpka, Karel - Kozubek, Michal - Navrátilová, Jarmila - Pavlatovská, Barbora - Machálková, Markéta - Preisler, Jan - Pruška, Adam
PY - 2019
TI - Quantitative Assessment of Anti-Cancer Drug Efficacy From Coregistered Mass Spectrometry and Fluorescence Microscopy Images of Multicellular Tumor Spheroids
JF - Microscopy and Microanalysis
VL - 25
IS - 6
SP - 1311-1322
EP - 1311-1322
PB - Cambridge University Press
SN - 14319276
KW - confocal microscopy
KW - image registration
KW - MALDI MS
KW - mass spectrometry imaging
KW - peeling
UR - http://dx.doi.org/10.1017/S1431927619014983
L2 - http://dx.doi.org/10.1017/S1431927619014983
N2 - Spheroids—three-dimensional aggregates of cells grown from a cancer cell line—represent a model of living tissue for chemotherapy inves- tigation. Distribution of chemotherapeutics in spheroid sections was determined using the matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI). Proliferating or apoptotic cells were immunohistochemically labeled and visualized by laser scanning confocal fluorescence microscopy (LSCM). Drug efficacy was evaluated by comparing coregistered MALDI MSI and LSCM data of drug- treated spheroids with LSCM only data of untreated control spheroids. We developed a fiducial-based workflow for coregistration of low- resolution MALDI MS with high-resolution LSCM images. To allow comparison of drug and cell distribution between the drug-treated and untreated spheroids of different shapes or diameters, we introduced a common diffusion-related coordinate, the distance from the spheroid boundary. In a procedure referred to as “peeling”, we correlated average drug distribution at a certain distance with the average reduction in the affected cells between the untreated and the treated spheroids. This novel approach makes it possible to differentiate between peripheral cells that died due to therapy and the innermost cells which died naturally. Two novel algorithms—for MALDI MS image denoising and for weighting of MALDI MSI and LSCM data by the presence of cell nuclei—are also presented.
ER -
MICHÁLEK, Jan, Karel ŠTĚPKA, Michal KOZUBEK, Jarmila NAVRÁTILOVÁ, Barbora PAVLATOVSKÁ, Markéta MACHÁLKOVÁ, Jan PREISLER and Adam PRUŠKA. Quantitative Assessment of Anti-Cancer Drug Efficacy From Coregistered Mass Spectrometry and Fluorescence Microscopy Images of Multicellular Tumor Spheroids. \textit{Microscopy and Microanalysis}. Cambridge University Press, 2019, vol.~25, No~6, p.~1311-1322. ISSN~1431-9276. Available from: https://dx.doi.org/10.1017/S1431927619014983.
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