Detailed Information on Publication Record
2019
Quantitative Assessment of Anti-Cancer Drug Efficacy From Coregistered Mass Spectrometry and Fluorescence Microscopy Images of Multicellular Tumor Spheroids
MICHÁLEK, Jan, Karel ŠTĚPKA, Michal KOZUBEK, Jarmila NAVRÁTILOVÁ, Barbora PAVLATOVSKÁ et. al.Basic information
Original name
Quantitative Assessment of Anti-Cancer Drug Efficacy From Coregistered Mass Spectrometry and Fluorescence Microscopy Images of Multicellular Tumor Spheroids
Authors
MICHÁLEK, Jan (203 Czech Republic, guarantor, belonging to the institution), Karel ŠTĚPKA (203 Czech Republic, belonging to the institution), Michal KOZUBEK (203 Czech Republic, belonging to the institution), Jarmila NAVRÁTILOVÁ (203 Czech Republic, belonging to the institution), Barbora PAVLATOVSKÁ (203 Czech Republic, belonging to the institution), Markéta MACHÁLKOVÁ (203 Czech Republic, belonging to the institution), Jan PREISLER (203 Czech Republic, belonging to the institution) and Adam PRUŠKA (203 Czech Republic, belonging to the institution)
Edition
Microscopy and Microanalysis, Cambridge University Press, 2019, 1431-9276
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
20602 Medical laboratory technology ;
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 3.414
RIV identification code
RIV/00216224:14330/19:00110913
Organization unit
Faculty of Informatics
UT WoS
000502313700003
Keywords in English
confocal microscopy; image registration; MALDI MS; mass spectrometry imaging; peeling
Tags
International impact, Reviewed
Změněno: 15/1/2021 08:47, Mgr. Marie Šípková, DiS.
Abstract
V originále
Spheroids—three-dimensional aggregates of cells grown from a cancer cell line—represent a model of living tissue for chemotherapy inves- tigation. Distribution of chemotherapeutics in spheroid sections was determined using the matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI). Proliferating or apoptotic cells were immunohistochemically labeled and visualized by laser scanning confocal fluorescence microscopy (LSCM). Drug efficacy was evaluated by comparing coregistered MALDI MSI and LSCM data of drug- treated spheroids with LSCM only data of untreated control spheroids. We developed a fiducial-based workflow for coregistration of low- resolution MALDI MS with high-resolution LSCM images. To allow comparison of drug and cell distribution between the drug-treated and untreated spheroids of different shapes or diameters, we introduced a common diffusion-related coordinate, the distance from the spheroid boundary. In a procedure referred to as “peeling”, we correlated average drug distribution at a certain distance with the average reduction in the affected cells between the untreated and the treated spheroids. This novel approach makes it possible to differentiate between peripheral cells that died due to therapy and the innermost cells which died naturally. Two novel algorithms—for MALDI MS image denoising and for weighting of MALDI MSI and LSCM data by the presence of cell nuclei—are also presented.
Links
| EF16_013/0001775, research and development project |
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| LM2015062, research and development project |
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| LQ1601, research and development project |
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| LTC17016, research and development project |
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| MUNI/A/1359/2018, interní kód MU |
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| MUNI/G/0974/2016, interní kód MU |
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