Detailed Information on Publication Record
2019
The Effect of Uncoated SPIONs on hiPSC-Differentiated Endothelial Cells
ŠALINGOVÁ, Barbara, Pavel ŠIMARA, Pavel MATULA, Lenka ZAJÍČKOVÁ, Petr SYNEK et. al.Basic information
Original name
The Effect of Uncoated SPIONs on hiPSC-Differentiated Endothelial Cells
Authors
ŠALINGOVÁ, Barbara (203 Czech Republic, belonging to the institution), Pavel ŠIMARA (203 Czech Republic, belonging to the institution), Pavel MATULA (203 Czech Republic, belonging to the institution), Lenka ZAJÍČKOVÁ (203 Czech Republic, belonging to the institution), Petr SYNEK (203 Czech Republic, belonging to the institution), Ondřej JAŠEK (203 Czech Republic, belonging to the institution), Lenka VEVERKOVÁ (203 Czech Republic, belonging to the institution), Miroslava SEDLÁČKOVÁ (203 Czech Republic, belonging to the institution), Zuzana NICHTOVA (703 Slovakia) and Irena KOUTNÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
International Journal of Molecular Sciences, BASEL, Molecular Diversity Preservation International, 2019, 1422-0067
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30402 Technologies involving the manipulation of cells, tissues, organs or the whole organism
Country of publisher
Switzerland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 4.556
RIV identification code
RIV/00216224:14110/19:00108522
Organization unit
Faculty of Medicine
UT WoS
000480449300160
Keywords in English
superparamagnetic iron-oxide nanoparticles; human induced pluripotent stem cell-derived endothelial cells; reprogramming; differentiation; mature endothelial cells
Tags
International impact, Reviewed
Změněno: 11/10/2022 12:50, Mgr. Marie Šípková, DiS.
Abstract
V originále
Background: Endothelial progenitor cells (EPCs) were indicated in vascular repair, angiogenesis of ischemic organs, and inhibition of formation of initial hyperplasia. Differentiation of endothelial cells (ECs) from human induced pluripotent stem cells (hiPSC)-derived endothelial cells (hiPSC-ECs) provides an unlimited supply for clinical application. Furthermore, magnetic cell labelling offers an effective way of targeting and visualization of hiPSC-ECs and is the next step towards in vivo studies. Methods: ECs were differentiated from hiPSCs and labelled with uncoated superparamagnetic iron-oxide nanoparticles (uSPIONs). uSPION uptake was compared between hiPSC-ECs and mature ECs isolated from patients by software analysis of microscopy pictures after Prussian blue cell staining. The acute and long-term cytotoxic effects of uSPIONs were evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay) and Annexin assay. Results: We showed, for the first time, uptake of uncoated SPIONs (uSPIONs) by hiPSC-ECs. In comparison with mature ECs of identical genetic background hiPSC-ECs showed lower uSPION uptake. However, all the studied endothelial cells were effectively labelled and showed magnetic properties even with low labelling concentration of uSPIONs. uSPIONs prepared by microwave plasma synthesis did not show any cytotoxicity nor impair endothelial properties. Conclusion: We show that hiPSC-ECs labelling with low concentration of uSPIONs is feasible and does not show any toxic effects in vitro, which is an important step towards animal studies.
Links
| NV16-31501A, research and development project |
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