The effect of Betanin parenteral pretreatment on Jejunal and
pulmonary tissue histological architecture and inflammatory
response after Jejunal ischemia-reperfusion injury

J 2019

The effect of Betanin parenteral pretreatment on Jejunal and pulmonary tissue histological architecture and inflammatory response after Jejunal ischemia-reperfusion injury

TOTH, Stefan, Zuzana JONECOVA, Milan MARETTA, Kristina CURGALI, Theodoros KALPAKIDIS et. al.

Basic information

Original name

The effect of Betanin parenteral pretreatment on Jejunal and pulmonary tissue histological architecture and inflammatory response after Jejunal ischemia-reperfusion injury

Authors

TOTH, Stefan (703 Slovakia), Zuzana JONECOVA (703 Slovakia), Milan MARETTA (703 Slovakia), Kristina CURGALI (703 Slovakia), Theodoros KALPAKIDIS (703 Slovakia), Martin PRIBULA (703 Slovakia), Matus KUSNIER (703 Slovakia), Zuzana FAGOVA (703 Slovakia), Julia FEDOTOVA (643 Russian Federation), Giampiero LA ROCCA (380 Italy), Luis RODRIGO (724 Spain), Martin CAPRNDA (703 Slovakia), Anthony ZULLI (36 Australia), Rachele CICCOCIOPPO (380 Italy), Eva MECHIROVA (703 Slovakia) and Peter KRUŽLIAK (703 Slovakia, guarantor, belonging to the institution)

Edition

Experimental and Molecular Pathology, USA, The Human Press, 2019, 0014-4800

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30109 Pathology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.280

RIV identification code

RIV/00216224:14110/19:00110980

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.yexmp.2019.104292

UT WoS

000488144100024

Keywords in English

Betanin; Ischemia-reperfusion injury; Jejunum; Lung; Lung injury index; Mast cell; Mucosal injury index; Myeloperoxidase

Abstract

V originále

Intestinal ischemic-reperfusion (IR) injury has detrimental effects on both local and distant organs in the body. Betanin is known for its antioxidant properties, and it is found mostly in vegetables. Therefore, the aim of the present study was to test the hypothesis that betanin administration prior intestinal IR, may be beneficial in protecting jejuna! mucosa and lung parenchyma against IR damage. Male specific pathogen-free Charles River Wistar rats were used (n = 42). Betanin (50 mg/kg) was administered intraperitoneally 30 min before ischemia of the superior mesenteric artery lasting 1 h, followed by 1, 4 and 24 h of reperfusion. Immunohistochemical as well as histomorphometrical analysis indicated a protective effect of betanin pretreatment on jejunal tissue. Regarding morphometrical analysis betanin significantly (p < 0.01) augments intestinal villus height after 24 of reperfusion comparing to early stages. Betanin application reduced number of mast cells population in early reperfusion periods (p < 0.05). The protective effect of betanin on lung parenchyma, was detected in late reperfusion period (24 h) with improvement of histopathological injury index and morphometric analysis (p < 0.001 for both). The improvement of histopathological injury index (p < 0.001) and morphometric analysis (p < 0.001) during the late reperfusion period, suggests a protective effect of betanin on lung parenchyma. Moreover, suppression of the inflammatory response was mirrored by the reduction of myeloperoxidase (MPO) positive cells within lung parenchyma after 1 and 4 h of reperfusion (p < 0.001). Especially, during the first 4 h of reperfusion after betanin administration, a reduction of 74% of the polymorphonuclear neutrophils infiltration (MPO positive cell population) and of a nearly 46% of active MCs was observed. Upon morphometric examination, the lung histological architecture after 24 h of reperfusion appeared to be almost 100% better following betanin treatment, with 25% thinner interalveolar septa and 20% larger alveolar surface for respiratory gas exchange. The results suggest that betanin pretreatment protects the jejunal mucosa and the lung parenchyma, as well as reduces the inflammatory cell density after intestinal IR injury.

Citovat

TOTH, Stefan, Zuzana JONECOVA, Milan MARETTA, Kristina CURGALI, Theodoros KALPAKIDIS, Martin PRIBULA, Matus KUSNIER, Zuzana FAGOVA, Julia FEDOTOVA, Giampiero LA ROCCA, Luis RODRIGO, Martin CAPRNDA, Anthony ZULLI, Rachele CICCOCIOPPO, Eva MECHIROVA and Peter KRUŽLIAK. The effect of Betanin parenteral pretreatment on Jejunal and pulmonary tissue histological architecture and inflammatory response after Jejunal ischemia-reperfusion injury. Experimental and Molecular Pathology. USA: The Human Press, 2019, vol. 110, OCT 2019, p. 1-9. ISSN 0014-4800. Available from: https://dx.doi.org/10.1016/j.yexmp.2019.104292.

@article{1570376,
   author = {Toth, Stefan and Jonecova, Zuzana and Maretta, Milan and Curgali, Kristina and Kalpakidis, Theodoros and Pribula, Martin and Kusnier, Matus and Fagova, Zuzana and Fedotova, Julia and La Rocca, Giampiero and Rodrigo, Luis and Caprnda, Martin and Zulli, Anthony and Ciccocioppo, Rachele and Mechirova, Eva and Kružliak, Peter},
   article_location = {USA},
   article_number = {OCT 2019},
   doi = {http://dx.doi.org/10.1016/j.yexmp.2019.104292},
   keywords = {Betanin; Ischemia-reperfusion injury; Jejunum; Lung; Lung injury index; Mast cell; Mucosal injury index; Myeloperoxidase},
   language = {eng},
   issn = {0014-4800},
   journal = {Experimental and Molecular Pathology},
   title = {The effect of Betanin parenteral pretreatment on Jejunal and pulmonary tissue histological architecture and inflammatory response after Jejunal ischemia-reperfusion injury},
   url = {http://dx.doi.org/10.1016/j.yexmp.2019.104292},
   volume = {110},
   year = {2019}
}

TY  - JOUR
ID  - 1570376
AU  - Toth, Stefan - Jonecova, Zuzana - Maretta, Milan - Curgali, Kristina - Kalpakidis, Theodoros - Pribula, Martin - Kusnier, Matus - Fagova, Zuzana - Fedotova, Julia - La Rocca, Giampiero - Rodrigo, Luis - Caprnda, Martin - Zulli, Anthony - Ciccocioppo, Rachele - Mechirova, Eva - Kružliak, Peter
PY  - 2019
TI  - The effect of Betanin parenteral pretreatment on Jejunal and pulmonary tissue histological architecture and inflammatory response after Jejunal ischemia-reperfusion injury
JF  - Experimental and Molecular Pathology
VL  - 110
IS  - OCT 2019
SP  - 1-9
EP  - 1-9
PB  - The Human Press
SN  - 00144800
KW  - Betanin
KW  - Ischemia-reperfusion injury
KW  - Jejunum
KW  - Lung
KW  - Lung injury index
KW  - Mast cell
KW  - Mucosal injury index
KW  - Myeloperoxidase
UR  - http://dx.doi.org/10.1016/j.yexmp.2019.104292
L2  - http://dx.doi.org/10.1016/j.yexmp.2019.104292
N2  - Intestinal ischemic-reperfusion (IR) injury has detrimental effects on both local and distant organs in the body. Betanin is known for its antioxidant properties, and it is found mostly in vegetables. Therefore, the aim of the present study was to test the hypothesis that betanin administration prior intestinal IR, may be beneficial in protecting jejuna! mucosa and lung parenchyma against IR damage. Male specific pathogen-free Charles River Wistar rats were used (n = 42). Betanin (50 mg/kg) was administered intraperitoneally 30 min before ischemia of the superior mesenteric artery lasting 1 h, followed by 1, 4 and 24 h of reperfusion. Immunohistochemical as well as histomorphometrical analysis indicated a protective effect of betanin pretreatment on jejunal tissue. Regarding morphometrical analysis betanin significantly (p < 0.01) augments intestinal villus height after 24 of reperfusion comparing to early stages. Betanin application reduced number of mast cells population in early reperfusion periods (p < 0.05). The protective effect of betanin on lung parenchyma, was detected in late reperfusion period (24 h) with improvement of histopathological injury index and morphometric analysis (p < 0.001 for both). The improvement of histopathological injury index (p < 0.001) and morphometric analysis (p < 0.001) during the late reperfusion period, suggests a protective effect of betanin on lung parenchyma. Moreover, suppression of the inflammatory response was mirrored by the reduction of myeloperoxidase (MPO) positive cells within lung parenchyma after 1 and 4 h of reperfusion (p < 0.001). Especially, during the first 4 h of reperfusion after betanin administration, a reduction of 74% of the polymorphonuclear neutrophils infiltration (MPO positive cell population) and of a nearly 46% of active MCs was observed. Upon morphometric examination, the lung histological architecture after 24 h of reperfusion appeared to be almost 100% better following betanin treatment, with 25% thinner interalveolar septa and 20% larger alveolar surface for respiratory gas exchange. The results suggest that betanin pretreatment protects the jejunal mucosa and the lung parenchyma, as well as reduces the inflammatory cell density after intestinal IR injury.
ER  -

TOTH, Stefan, Zuzana JONECOVA, Milan MARETTA, Kristina CURGALI, Theodoros KALPAKIDIS, Martin PRIBULA, Matus KUSNIER, Zuzana FAGOVA, Julia FEDOTOVA, Giampiero LA ROCCA, Luis RODRIGO, Martin CAPRNDA, Anthony ZULLI, Rachele CICCOCIOPPO, Eva MECHIROVA and Peter KRUŽLIAK. The effect of Betanin parenteral pretreatment on Jejunal and pulmonary tissue histological architecture and inflammatory response after Jejunal ischemia-reperfusion injury. \textit{Experimental and Molecular Pathology}. USA: The Human Press, 2019, vol.~110, OCT 2019, p.~1-9. ISSN~0014-4800. Available from: https://dx.doi.org/10.1016/j.yexmp.2019.104292.

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